Severe corneal burn due to the accidental application of salicylic acid packed in a plastic dropper bottle

Abstract Eye burns due to the accidental application of pharmacological or nonpharmacological substances packaged in plastic dropper bottles have been described for more than three decades and continue to occur. These burns can cause potentially serious corneal injuries. We report the case of a patient who mistakenly applied salicylic acid to the right eye after confusing it with an eye lubricant, which caused him a severe corneal burn. Fortunately, after aggressive medical and surgical management (including oxygen therapy and amniotic membrane grafting), the visual results were good. We suggest conducting educational campaigns and taking legislative measures in our country to avoid packaging corrosive substances in this type of dropper bottle to reduce the risk of accidental burns.

Chemical eye burns constitute a true ophthalmological emergency and are responsible for 1.3% to 6.4% of emergency consultations for ocular causes (1). They must be treated immediately and can have a devastating impact on the patient's vision and quality of life. Therefore, the establishment of preventive measures and strategies to reduce the frequency of these accidents in the future is vital.
Approximately two-thirds of eye burns occur with chemicals at work; however, another cause of eye burns that has been described since the 1970s and, unfortunately, continues to occur is when a corrosive element is accidentally applied in the eyes after confusing it with ophthalmic eye drops given the similarity of the bottles containing them. This same mistake can be made by a patient or by health personnel (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13).
We describe the case of an adult patient who presented an eye burn due to this type of accident.

Clinical case
A 59-year-old male patient arrived at the emergency department of the Fundación Oftalmológica de Santander -Foscal in 2017 after having accidentally applied on his right eye some drops that he used as an antifungal. These drops, manufactured by a laboratory with no approval from the drugs regulatory agency in Colombia (Instituto Nacional de Vigilancia de Medicamentos y Alimentos, Invima), apparently contained salicylic acid and were packaged in a plastic dropper bottle. The accident occurred because he confused the bottle with one containing eye lubricant.
Upon admission, three hours after the event, palpebral soft edema, inferior chemosis, and staining in some areas of the bulbar conjunctiva were found in the affected eye. A central abrasion area of approximately 4 x 4 mm was visible in the cornea and the rest of it was covered by a white-grayish membrane of necrotic epithelial tissue. The tarsal conjunctiva was also covered by a similar membrane. Cellularity was found in the anterior chamber (+) and medium mydriasis.
Profuse washing and subtotal removal of the corneal and conjunctival necrotic material were performed (the necrotic tissue was firmly attached to the corneal periphery).
At assessment six hours later, we found almost total de-epithelization of the cornea and a membrane of necrotic tissue 360 degrees around the peripheral cornea and the adjacent limbus. The de-epithelized area showed very little fluorescein uptake (figure 1). The absence of epithelium was confirmed by attempting a debridement of the central area of the cornea with forceps, which evidenced a hardened membrane seemingly corresponding to a Bowman's membrane with changes in protein coagulation (figure 2).

Figure 1.
Six hours after the acid burn and after debridement of the necrotic tissue membrane covering the cornea, a remnant could be seen in the entire corneal periphery (white arrows). The de-epithelized area of the cornea is stained very slightly with fluorescein. In the remnant of the peripheral necrotic membrane, obvious fluorescence was observed when using the cobalt blue filter (right).
Given the severity of the condition, an emergency surgical procedure was performed the next day with an amniotic membrane graft covering the entire ocular surface and supported by a retention ring made of intravenous tubing (14). On the first postoperative day, the retention ring presented displacement and extrusion with the loss of the membrane. A new graft was performed with a double amniotic membrane fixed with tissue glue (fibrin sealant) and anchored to the membrane using 6-0 polypropylene mattress sutures and bolsters. A total of 0.4 ml of autologous plasma enriched with platelet growth factors, antiangiogenic (12.5 mg bevacizumab) and steroid (20 mg triamcinolone) were also delivered through subconjunctival injection (figure 3).
One week after the burn, one-hour daily 100% oxygen therapy via a face mask at 10 L/min was started (16 sessions). Besides, six sessions of 5 L/min of oxygen application were performed on the right eye with a camera made for that purpose (15,16). Twenty days after the burn, the amniotic membrane had been almost completely reabsorbed, with only a remnant observed on the temporal conjunctiva, and 95% of the cornea was epithelialized with only two remaining areas with fluorescein staining (figure 4).
After one month, the biconjugate of gatifloxacin and dexamethasone was changed to 0.5% loteprednol (Lotesoft™, Poen) in a two-month taper scheme. On examination, there was a central cornea with haze, inferior punctate keratitis, and mild conjunctivalization between the 9 and 10 o'clock meridians. The corneal haze decreased progressively in the following months reaching  In March, 2018, (seven months after the burn), the clinical picture was stable and good central corneal transparency (without fluorescein staining) and a slight decrease of conjunctivalization were observed. Preservative-free lubricants were prescribed.
In the last control, two years after the burn, conjunctivalization was evident only in two quadrants (figure 6). The cornea had good transparency and showed no fluorescein staining. Visual acuity was found in 20/40 without correction and reached 20/20 with + 0.50-0.50 x 90.

Discussion
Corneal burns can be caused by chemical substances (solid, liquid, dust, or vapor) when they come into contact with the ocular surface. These chemicals can be alkalis or acids contained in several products including detergents, disinfectants, and solvents. Generally, the lesions caused by alkalis (ammonia, magnesium hydroxide, lime, and bleach) are more serious than those caused by acids (sulfuric, hydrochloric, and nitrous) because they have a greater capacity to penetrate tissues (17,18).
Immediate copious irrigation, even using regular tap water, is universally recommended in acute eye burns preferably at the moment of the accident at home or in the workplace. Eye washing should be continued in emergency wards (18,19). Medical therapy involves the use of agents to promote epithelialization, minimize inflammation, and prevent scar complications. Biological fluids including umbilical cord blood serum, amniotic membrane suspension, autologous serum, and autologous plasma rich in growth factors promote healing when used as supplements of conventional therapy (18,19). Surgical treatment of acute eye burns includes debridement of the necrotic tissue, application of tissue adhesives (fibrin glue), tenoplasty, and tectonic keratoplasty. Amniotic membrane transplantation is a surgical treatment frequently used as a complement of the conventional treatment to promote epithelial healing, minimize pain, and restore visual acuity. Although many cases with positive results have been published, clear evidence of its positive effect is scarce (18)(19)(20). Another rarely used alternative is systemic or local oxygen therapy as proposed by Sharifipour, et al. (15,16). In our case, we obtained good results by adding to the medical management both the use of the amniotic membrane and oxygen therapy using masks or cameras over the eye.
A finding that caught our attention was that, initially, the examination did not show a clear uptake of fluorescein on the corneal surface, which was almost completely devoid of epithelium; this was confirmed when we scraped with forceps in the central area and were not able to lift any cell layer that could correspond to the epithelium (figures 2 and 3). This may be explained because due to the coagulation necrosis caused by the acid, the epithelium became a white-grayish membrane that was lost in 90% of the corneal area with only a peripheral remnant, not viable, over 2 mm of peripheral cornea, remaining ( figure 1) (17). The area thus exposed very possibly corresponded to a Bowman's membrane that, due to the severe changes in its protein coagulation, stained very little with fluorescein. We have seen this in a couple of cases of severe acid burns. To the best of our knowledge, this finding has not been reported, although there is no doubt that those frequently receiving patients with corneal acid burns have seen it. However, someone with little experience can be confounded by this and conclude that there is no corneal epithelial compromise because a clear uptake of fluorescein is not visible. However, there might be a total compromise of the corneal epithelium and, therefore, it is something that should be considered.
Despite having a favorable evolution, our patient secondarily developed partial limbal insufficiency due to the severity of the incident, which fortunately remained stable.
The case we report and other similar ones previously published demonstrate the danger of packaging caustic substances in squeezable dropper bottles that can be mistakenly confused with those containing ophthalmic medications ( figure 7). The American Academy of Ophthalmology has a policy statement recommending uniform color-coding of caps and labels for topical eye medications (21); surprisingly, to the best of our knowledge, currently there is no standardized policy to avoid packaging dangerous pharmacological or nonpharmacological substances for the eyes in squeezable plastic dropper bottles similar to those used for eye medications. We plan to initiate an awareness campaign in this regard both for the general public through the media and in scientific publications focused on health professionals starting with the present report to engage them in the prevention of these accidents and in advising patients to store medications in completely separate places from nonpharmacological substances in similar containers. Additionally, we will resume the work that we started a few years ago to ensure that the regulations in our country prohibit the packaging of dangerous substances in squeezable plastic dropper bottles that can be confused with ophthalmic eye drops.
Surprisingly, there are many unauthorized presentations of salicylic acid in this type of soft plastic container, as well as several commercial presentations of drugs for dermatological topical use that contain this caustic substance and have been authorized by the Colombian regulatory body (Invima) in this sort of packaging (plastic dropper bottles). Other manufacturers have modified their presentation and do so in glass bottles with brushes or spatulas attached to the lid, which avoids the risk of accidental application on the eyes. If a manufacturer argues that it is essential to pack a hazardous substance in a dropper bottle, then it should be mandatory to use glass bottles and droppers. If plastic must be used, the shape of the lid should be changed (including, for Figure 7. Squeezable plastic dropper bottles containing salicylic acid produced by informal laboratories can be used as keratolytic or antifungal substances for dermatological conditions. However, these bottles can easily be confused with a dropper bottle for ophthalmic medication. instance, a truncated cylinder-shaped end) so that it is easy to differentiate them from eye drops containers. Additionally, these plastic bottles should have a safety cap with safety locks to avoid child manipulation and patients confusing them with eye medication.
Scientific articles have been suggesting such measures for more than 30 years; however, in many cases they have been ignored and, therefore, we continue to receive cases that are completely preventable (21)(22)(23).