Expresión diferencial de proteínas en Leishmania (Viannia) panamensis asociadas con mecanismos de resistencia a antimoniato de meglumina

Ronald Guillermo Peláez, Carlos Enrrique Muskus, Patricia Cuervo, Marcel Marín-Villa, .

Resumen

Introducción. Los mecanismos de resistencia al antimonio pentavalente conocidos hasta el momento,se han descrito ampliamente en cepas del subgénero Leishmania, pero poco se sabe sobre lasproteínas involucradas en los mecanismos de resistencia presentes en cepas del subgénero Viannia,como Leishmania panamensis.

Objetivo. Identificar proteínas diferencialmente expresadas entre las cepas de L. panamensis(UA140), sensible y resistente al antimonio pentavalente, y analizar el posible papel de estas proteínasen mecanismos de resistencia.

Materiales y métodos. Las proteínas de las cepas, sensible y resistente al antimonio pentavalente, secompararon usando electroforesis bidimensional. Las proteínas con aumento de la expresión fueronaisladas e identificadas por espectrometría de masas mediante MALDI-TOF/TOF (Matrix AssistedLaser Desorption Ionization/Time of Flight). La expresión del ARNm de cinco de estas proteínas secuantificó mediante PCR en tiempo real.

Resultados. Los geles bidimensionales de las cepas sensible y resistente detectaron 532±39 y 541±43manchas proteicas. Se encontraron 10 manchas con aumento de la expresión en la cepa resistente,identificadas como proteínas de choque térmico (Hsp60 mitocondrial, Hsp70 mitocondrial y citosólica),isomerasa de disulfuro, proteasa de cisteína, enolasa, factor de elongación 5-α, la subunidad 5-α delproteasoma y dos proteínas hipotéticas nombradas como Sp(2) y Sp(25).

Conclusión. Este es el primer estudio llevado a cabo con una cepa resistente al antimonio pentavalenteen L. panamensis, en el cual se han identificado proteínas que están relacionadas con el mecanismode resistencia del parásito frente al medicamento, abriendo el camino para futuros estudios de estasproteínas como blancos terapéuticos.

 

doi: http://dx.doi.org/10.7705/biomedica.v32i3.392

 

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  • Ronald Guillermo Peláez Programa de Estudio y Control de Enfermedades Tropicales, PECET, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia
  • Carlos Enrrique Muskus Programa de Estudio y Control de Enfermedades Tropicales, PECET, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia
  • Patricia Cuervo Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz-FIOCRUZ, Rio de Janeiro, Brasil
  • Marcel Marín-Villa Programa de Estudio y Control de Enfermedades Tropicales, PECET, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

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Cómo citar
1.
Peláez RG, Muskus CE, Cuervo P, Marín-Villa M. Expresión diferencial de proteínas en Leishmania (Viannia) panamensis asociadas con mecanismos de resistencia a antimoniato de meglumina. biomedica [Internet]. 2 de mayo de 2012 [citado 28 de marzo de 2024];32(3):418-29. Disponible en: https://revistabiomedica.org/index.php/biomedica/article/view/392
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2012-05-02
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