Caracterización molecular de las variantes del virus de Epstein-Barr detectadas en la cavidad oral de adolescentes de Cali, Colombia

Daniela Arturo-Terranova, Sebastián Giraldo-Ocampo, Andrés Castillo , .

Palabras clave: infecciones por virus de Epstein-Barr, boca, filogenia, adolescente, Colombia

Resumen

Introducción. El virus de Epstein-Barr (EBV) es un virus ubicuo y oncogénico, asociado con el desarrollo de enfermedades como la mononucleosis infecciosa, el linfoma de Burkitt, el carcinoma nasofaríngeo y otras neoplasias. Actualmente, se reconocen dos subtipos: EBV-1 y EBV- 2, que tienen diferencias genéticas con sus antígenos nucleares (Epstein-Barr Nuclear Antigens, EBNA). Debido a la gran heterogeneidad y variabilidad encontradas en la proteína LMP1 del virus, se han descrito variantes asociadas con ciertas enfermedades o con regiones geográficas específicas.
Objetivo. Identificar y caracterizar molecularmente las variantes del EBV detectadas en la cavidad oral de 84 adolescentes de Cali, Colombia.
Materiales y métodos. Se hizo la amplificación por reacción en cadena de la polimerasa (Polymerase Chain Reaction, PCR) convencional, así como la purificación y la secuenciación del gen EBNA3C se realizó para subtipificar el virus y del dominio C-ter de la proteína LMP1 para identificar variantes. Además, se llevó a cabo un análisis filogenético y de variantes nucleotídicas de las secuencias obtenidas comparadas con variantes patogénicas y geográficas reportadas en el GenBank (National Center for Biotechnology Information, NCBI).
Resultados. El subtipo viral predominante fue el EBV-1 (79 %); el 72,6 % se agrupó con la variante patogénica Raji, derivada de linfocitos B de un paciente con linfoma de Burkitt; el 13,7 % se relacionó con una variante de origen geográfico del Mediterráneo y otro 13,7 % no se agrupó con ninguna de las variantes de referencia.
Conclusiones. Este es el primer estudio que reporta variantes del gen LMP1-EBV en Cali, Colombia. Se requieren nuevos estudios para caracterizar la variante sin identificar y determinar si es patogénica o si es una variante geográfica presente exclusivamente en la ciudad.

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  • Daniela Arturo-Terranova Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali, Colombia; Posgrado en Ciencias Biomédicas, Escuela de Ciencias Básicas, Facultad de Salud, Universidad del Valle, Cali, Colombia http://orcid.org/0000-0002-5914-0601
  • Sebastián Giraldo-Ocampo Posgrado en Ciencias Biomédicas, Escuela de Ciencias Básicas, Facultad de Salud, Universidad del Valle, Cali, Colombia
  • Andrés Castillo Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali, Colombia; Posgrado en Ciencias Biomédicas, Escuela de Ciencias Básicas, Facultad de Salud, Universidad del Valle, Cali, Colombia

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Cómo citar
Arturo-Terranova, D., Giraldo-Ocampo, S., & Castillo , A. (2020). Caracterización molecular de las variantes del virus de Epstein-Barr detectadas en la cavidad oral de adolescentes de Cali, Colombia. Biomédica, 40(Supl. 1), 76-88. https://doi.org/10.7705/biomedica.4917
Publicado
2020-05-01