Actividad antinflamatoria de extractos y fracciones obtenidas de cálices de Physalis peruviana L.
Luis A. Franco, Germán E. Matiz, Jairo Calle, Roberto Pinzón, Luis F. Ospina, .
Resumen
Introducción. Los cálices de la uchuva, Physalis peruviana, son ampliamente utilizados en la medicina popular por sus propiedades como anticancerígeno, antimicobacterial, antipirético, diurético, immunomodulador y antinflamatorio.Objetivo. Evaluar el efecto antinflamatorio de extractos y fracciones obtenidas de los cálices de Physalis peruviana en un modelo de inflamación aguda en ratón, procurando identificar las fracciones responsables de dicha actividad.
Materiales y métodos. Los cálices de Physalis peruviana fueron extraídos por percolación con solventes orgánicos. La fracción primaria hidroalcohólica se purificó mediante cromatografía en columna. La actividad antinflamatoria de los extractos y fracciones se evaluó utilizando el modelo murino de edema auricular inducido por 13-acetato de 12-tetradecanoilforbol.
Resultados. Se obtuvieron 38 fracciones secundarias en la cromatografía en columna de la fracción primaria hidroalcohólica, de las cuales seis fueron evaluadas en el modelo inflamatorio, mostrando actividad significativa (p<0,05). La fracción mayoritaria identificada como Pp-D28-LF presentó una respuesta dosis dependiente con significativa inhibición del edema en dosis superiores a 250 μg/oreja (p<0,05).
Conclusión. Este estudio confirma la actividad antinflamatoria atribuida a los cálices de Physalis peruviana y también valida su uso en la medicina popular. Se identificaron las principales fracciones responsables de la actividad antinflamatoria, las cuales parecen ser promisorias para el desarrollo de fitopreparados. Se requieren estudios posteriores para aislar e identificar los compuestos responsables de la actividad y también para investigar el mecanismo involucrado en el efecto antinflamatorio observado.
Descargas
- Luis A. Franco Departamento de Farmacia, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Cartagena, Cartagena, Colombia.
- Germán E. Matiz Departamento de Farmacia, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Cartagena, Cartagena, Colombia.
- Jairo Calle Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá D.C., Colombia.
- Roberto Pinzón Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia.
- Luis F. Ospina Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia
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