Flow cytometry and fluorescence-activated cell sorting in plants: the past, present, and future

David W. Galbraith, .

Resumen

Introduction: Flow cytometry and cell sorting are powerful technologies for examining the molecular, genetic, and physiological properties of individual cells.Objective: The objective of this article is to provide a historical survey of the development of flow cytometry and cell sorting for use with higher plants, a summary of the state of art at the present day, and a prediction of where the field might progress over the coming years.Methods: Adapting flow cytometry and sorting for use with higher plants requires the production of single cell suspensions, or suspensions of subcellular organelles. It also requires identification of methods for fluorescence labeling of the cells or organelles of interest, such that they can be usefully analyzed and sorting. These methods are identified and outlined.Results and conclusions: Recent advances in molecular and biotechnological methods, platforms, and instrumentation, combined with flow cytometry and sorting, provide increasingly powerful analytical tools for exploring the components and structure of regulatory networks governing plant growth and development, and the interactions of plants with their environments. They also will be invaluable in cataloguing the individual species that comprise the biological diversity of flowering plants.

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  • David W. Galbraith University of Arizona, School of Plant Sciences and Bio5 Institute for Collaborative Bioresearch

Referencias bibliográficas

Chattopadhyay PK, Hogerkorp CM, Roederer M. A chromatic explosion: the development and future of multiparameter flow cytometry. Immunology. 2008;125:441-9.

Heller FO. DNA measurement of Vicia faba L. with pulse cytophotometry. Ber Dtsch Bot Ges. 1973;86:437-41.

Galbraith DW, Harkins KR, Maddox JR, Ayres NM, Sharma DP, Firoozabady, E. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science. 1983;220:1049-51.

Galbraith DW. Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values. Cytometry. 2009;75A:692-8.

Sliwinska E, Pisarczyk I, Pawlik A, Galbraith DW. Measuring genome size of desert plants using dry seeds. Botany. 2009;87:127-35.

Galbraith DW, Bartos J, Dolezel J. Flow cytometry and cell sorting in plant biotechnology. In: Sklar LA, editor. Flow cytometry in biotechnology. Oxford: Oxford University Press; 2005. p. 291-322.

Harkins KR, Galbraith DW. Flow sorting and culture of plant protoplasts. Physiol Plant. 1984;60:43-52.

Harkins KR, Galbraith DW. Factors governing the flow cytometric analysis and sorting of large biological particles. Cytometry. 1987;8:60-71.

Afonso CL, Harkins KR, Thomas-Compton M, Krejci A, Galbraith DW. Production of somatic hybrid plants through fluorescence-activated sorting of protoplasts. Nat Biotechnol. 1985;3:811-6.

Harkins KR, Jefferson RA, Kavanagh TA, Bevan MW, Galbraith DW. Expression of photosynthesis related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts. Proc Natl Acad Sci USA. 1990;87:816-20.

Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science. 1994;263:802-5.

Sheen J, Hwang S, Niwa Y, Kobayashi H, Galbraith DW. Green fluorescent protein as a new vital marker in plant cells. Plant Journal. 1995;8:777-84.

Grebenok RJ, Lambert GM, Galbraith DW. Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants. Plant Journal. 1997;12:685-96.

Birnbaum K, Shasha DE, Wang JY, Jung JW, Lambert GM, Galbraith DW, et al. A gene expression map of the Arabidopsis root. Science. 2003;302:1956-60.

Zhang CQ, Gong FC, Lambert GM, Galbraith DW. Cell type-specific characterization of nuclear DNA contents within complex tissues and organs. Plant Methods. 2005;1:7.

Zhang CQ, Barthelson RA, Lambert GM, Galbraith DW. Characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. Plant Physiol. 2008;147:30-40.

Galbraith DW. Protoplast analysis using flow cytometry and sorting. In: Dolezel J, Greilhuber J, Suda J, editors. Flow cytometry with plant cells. New York: Wiley-VCH; 2007. p. 231-50.

Jackson PW, Kennedy K. The global strategy for plant conservation: a challenge and opportunity for the international community. Trends Plant Sci. 2009;14:578-80.

Nawy T, Lee J-Y, Colinas J, Wang JY, Thongrod SC, Malamy JE, et al. Transcriptional profile of the Arabidopsis root quiescent center. Plant Cell. 2005;17:1908-25.

Lee JY, Colinas J, Wang JY, Mace D, Ohler U, Benfey PN. Transcriptional and post-transcriptional regulation of transcription factor expression in Arabidopsis roots. Proc Natl Acad Sci USA. 2006;103:6055-60.

Brady SM, Orlando DA, Lee JY, Wang JY, Koch J, Dinneny JR, et al. A high-resolution root spatiotemporal map reveals dominant expression patterns. Science. 2007;318:801-6.

Dinneny JR, Long TA, Wang JY, Jung JW, Mace D, Pointer S, et al. Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science. 2008;320:942-5.

Yadav RK, Girke T, Pasala S, Xie MT, Reddy V. Gene expression map of the Arabidopsis shoot apical meristem stem cell niche. Proc Natl Acad Sci USA. 2009;106:4941-6.

Peterson SV, Johansson AI, Kowalczyk M, Makoveychuk A, Wang JY, Moritz, T et al. An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis. Plant Cell. 2009;21:1659-68.

Nelson BK, Cai X, Nebenführ A. A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants. Plant Journal. 2007;51:1126-36.

Geldner N, Denervaud-Tendon V, Hyman DL, Mayer U, Stierhof YD, Chory J. Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set. Plant Journal. 2009;59:169-78.

Bargmann BOR, Birnbaum KD. Positive fluorescent selection permits precise, rapid, and in-depth overexpression analysis in plant protoplasts. Plant Physiology. 2009;149:1231-9.

Raser JM, O’Shea EK. Control of stochasticity in eukaryotic gene expression. Science. 2004;304:1811-4.

Newman JRS, Ghaemmaghami S, Ihmels J, Breslow DK, Noble M, DeRisi JL, et al. Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise. Nature. 2006;441:840-6.

Tang FC, Barbacioru C, Wang Y, Nordman E, Lee C, Xu N, et al. mRNA-Seq whole-transcriptome analysis of a single cell. Nature Meth. 2009;6:377-U86.

Hawley TS, Telford WG, Ramezani A, Hawley RG. Four-color flow cytometric detection of retrovirally expressed red, yellow, green, and cyan fluorescent proteins. Biotechniques. 2001;30:1028-34.

Galbraith DW, Lucretti S. Large particle sorting. In: Radbruch A, editor. Flow cytometry and cell sorting. Second edition. Berlin: Springer-Verlag; 2000. p. 293-317.

Cómo citar
1.
Galbraith DW. Flow cytometry and fluorescence-activated cell sorting in plants: the past, present, and future. biomedica [Internet]. 28 de mayo de 2010 [citado 28 de marzo de 2024];30(Sup1):65-70. Disponible en: https://revistabiomedica.org/index.php/biomedica/article/view/824
Publicado
2010-05-28

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