Evaluation of a modified capillary technique for immunocytochemistry of coverslip adhered cultures

Abstract

In order to evaluate a simple and easy immunocytochemical method for cultured cell monolayers adhered to coverslips, we compared a minichamber technique with the conventional method using 24 well culture plates. lmmunocytochemical procedures were carried out on adult mice dorsal root ganglia cultures for fluorescence and peroxidase detection of neurofilament, S-100 protein or rabies virus. lntensity and especificity were similar using both techniques. Primary and secondary antibodies and enzymes volumes saving was 92% with similar reactivity and no increase in processing time of samples. This tecnique only requires materials routinely found in the labratory.