Variability of 6 Colombian strains of Trypanosoma cruzi with restriction fragment length polymorphisms (RFLP) and random amplification of polymorphic DNA (RAPD).

Pilar Rodríguez; Marcela Escalante; Hugo Díez; Claudia Cuervo; Marleny Montilla; Nicholls Rubén Santiago; Ignacio Zarante; Concepción Puerta

doi:10.7705/biomedica.v22i3.1163

Pilar Rodríguez, Marcela Escalante, Hugo Díez, Claudia Cuervo, Marleny Montilla, Nicholls Rubén Santiago, Ignacio Zarante, Concepción Puerta, .

DOI: https://doi.org/10.7705/biomedica.v22i3.1163

Keywords: trypanosoma cruzi, RAPD, RFLP, histone H2A, isoenzymes, polymorphism

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Abstract

Chagas disease, caused by the hemoflagellate Trypanosoma cruzi, is a public health problem in Colombia. Previous reports have indicated the presence of heterogeneity among parasite populations. Six Colombian T. cruzi strains were obtained that differed by host, geographical region and transmission cycle. The genetic variability of each was compared by random amplified polymorphic DNA (RAPD), and isoenzymes. A restriction fragment length polymorphism (RFLP) was extracted using the 1.2 kb unit encoding the parasite's H2A histone as a probe. Genetic distances between the isolates varied greatly, from 0.611 to 0.99 as determined by RAPD profiles (M13F and M13R primers), between 0 and 0.81 by RFLP profiles (5 endonucleases), and between 0.10 and 0.55 by isoenzymes (13 enzymatic systems). Genetic distance matrixes derived from each of the three methods showed that Colombian strains exhibit a high degree of genetic differentiation. This may account for the broad clinical spectrum of Chagas disease in Colombia.

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Pilar Rodríguez Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Bogotá, D.C.
Marcela Escalante Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Bogotá, D.C.
Hugo Díez Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Bogotá, D.C.
Claudia Cuervo Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Bogotá, D.C.
Marleny Montilla Laboratorio de Parasitología, Instituto Nacional de Salud, Bogotá, D.C.
Nicholls Rubén Santiago Laboratorio de Parasitología, Instituto Nacional de Salud, Bogotá, D.C.
Ignacio Zarante Instituto de Genética, Facultad de Medicina, Universidad Javeriana, Bogotá, D.C.
Concepción Puerta Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad de Ciencias, Universidad Javeriana, Bogotá, D.C.

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1.

Rodríguez P, Escalante M, Díez H, Cuervo C, Montilla M, Rubén Santiago N, et al. Variability of 6 Colombian strains of Trypanosoma cruzi with restriction fragment length polymorphisms (RFLP) and random amplification of polymorphic DNA (RAPD). biomedica [Internet]. 2002 Sep. 1 [cited 2024 May 19];22(3):263-71. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/1163

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Variability of 6 Colombian strains of Trypanosoma cruzi with restriction fragment length polymorphisms (RFLP) and random amplification of polymorphic DNA (RAPD).

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