Evaluation as a function of granulocytes in hyperimmunoglobulinemia E syndrome with recurrent infections.
Keywords:
hyper-IgE syndrome, GM-CSF, IL-5, chemotaxis, respiratory burst, adhesion molecules
Abstract
The hyper-IgE syndrome with recurrent infections (HIESRI) is characterized by skin and respiratory infections due to Staphylococcus aureus and several fungi infections which are frequently associated with tissue damage. A deficiency in the chemotaxis of phagocytic cells has been documented to explain these findings; however, the expression of adhesion molecules, the secretion of cytokines that activate granulocytes and the production of oxygen reactive molecules have not been evaluated in HIESRI. Six HIESRI patients were evaluated for the following parameters: (1) secretion of GM-CSF and IL-5 by mitogen and antigen-activated mononuclear cells, (2) the chemotactic response of FMLP-activated granulocytes, (3) the respiratory burst of PMA-activated granulocytes, and (4) the expression of L-selectin and CD11b in PMA-activated granulocytes. Human recombinant GM-CSF and culture supernatants were evaluated for capacity to modulate granulocytic function. Compared to controls, HIESRI patients showed a normal production of GM-CSF and an increase in the basal secretion of IL-5. No significant differences were observed for chemotaxis, respiratory burst or L-selectin and CD11b expression. The GM-CSF did not modulate these functions in granulocytes from HIESRI patients, but culture supernatants applied to granulocytes inhibited chemotaxis, increased respiratory burst and caused the shedding of L-selectin from the granulocyte surface. The 6 HIESRI patients were nonsymptomatic during the time of this research due to a program of continued treatment; findings suggest that granulocytes are activated more easily in response to proinflammatory factors and that production of these factors is higher in HIESRI.Downloads
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How to Cite
1.
Montoya CJ, López JA, Velilla PA, Rugeles C, Patiño PJ, García de Olarte DM. Evaluation as a function of granulocytes in hyperimmunoglobulinemia E syndrome with recurrent infections. Biomed. [Internet]. 2003 Mar. 1 [cited 2024 Aug. 18];23(1):60-76. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/1198
Some similar items:
- Carlos J. Montoya, Paula A. Velilla, Adriano M. Martínez, Juan C. Olarte, Modulation of L-selectin expression by chemotactic factors and a cytokine, GM-CSF , Biomedica: Vol. 22 No. 1 (2002)
Published
2003-03-01
Issue
Section
Original articles
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