Primary cultures of human umbilical chord vein endothelial cells: a biological model for studying enterococcal infection mechanisms.
Keywords:
Enterococcus sp., endothelium, cell culture, enterococcal infection, nosocomial infections
Abstract
Although enterococcus bacteria are normal human intestinal flora, they rank as the third most common pathogen involved in hospital acquired infections. Generally, these bacteria are considered extracellular pathogens; however, an increasing number of reports indicate invasiveness to epithelial cell lines and macrophages. Despite their importance as nosocomial infection agents in patients suffering bacteremias and endocarditis, their interaction with endothelial cells has not been fully described. Herein, the nosocomial Enterococcus faecalis isolate Ef2890 from a hospitalized patient was exposed to cultured human venous endothelial cells from the umbilical chord. When the primary cell cultures were inoculated with Ef2890 and treated with bactericidal antibiotics to kill extracellular and adhered bacteria, intracellular bacteria were recovered and plated 4 h post-infection. These observations indicate that cell cultures provide a valuable biological model to study interactions between endothelium and enterococci.Downloads
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How to Cite
1.
Chiriboga CA, Fontanilla MR. Primary cultures of human umbilical chord vein endothelial cells: a biological model for studying enterococcal infection mechanisms. biomedica [Internet]. 2004 Dec. 1 [cited 2024 May 18];24(4):456-63. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/1294
Published
2004-12-01
Issue
Section
Technical note
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