Photodynamic activity of aluminium (III) and zinc (II) phthalocyanines in Leishmania promastigotes
Patricia Escobar, Indira P. Hernández, Cesar M. Rueda, Fernando Martínez, Edgar Páez, .
Abstract
Introduction. Photodynamic therapy is a two-step procedure, involving the use ofphotosensitizing agents followed by selective illumination of the target lesion with visible light.It produces highly reactive oxygen species and subsequent cellular damage.
Objective. This study was designed to determine whether Leishmania chagasi and L.panamensis promastigotes were sensitive to photodynamic therapy in vitro.
Material and methods. Leishmania promastigotes were treated with aluminium phthalocyaninechloride and zinc phthalocyanine photosensitizers before illumination with visible light at 670nm. The parasite photoactivity was calculated by sigmoidal regression analysis.
Results. Leishmania chagasi promastigotes were highly photosensitive to aluminiumphthalocyanine chloride treatment with effective inhibitory dose50 (ED50) concentration values of0.0033, 0.0083 and 0.0093 μM upon exposure to 10.0, 5.0, and 2.5 J/cm2 light intensitiesrespectively. By contrast, the activity of aluminium phthalocyanine chloride on L. panamensiswas significantly lower (P<0.01) with ED50 values of 0.17, 0.25, 0.34 μM at the same lightintensities. Zinc phthalocyanine activity was significantly (P<0.01) less active than aluminiumphthalocyanine chloride on both strains of these two species and no differences in zincphthalocyanine activity were found between them. A dose-response phototoxic effect with bothphthalocyanines was observed. Parasite inhibition was not observed after aluminiumphthalocyanine chloride or zinc phthalocyanine treatment in the dark. The reference drugshexadecylphosphocholine and amphotericin B were not photoactive.
Conclusion. Treatment of Leishmania promastigotes with aluminium phthalocyanine chlorideand zinc phthalocyanine followed by illumination with visible light at 670 nm inhibited in vitrogrowth of promastigotes of L. chagasi and L. panamensis. Photodynamic therapy againstLeishmania could be a promising strategy for leishmaniasis treatment.
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- Patricia Escobar Centro de Investigación de Enfermedades Tropicales (CINTROP), Facultad de Salud, Escuela de Medicina, Departamento de Ciencias Básicas, Universidad Industrial de Santander, Bucaramanga, Colombia.
- Indira P. Hernández Centro de Investigación de Enfermedades Tropicales (CINTROP), Facultad de Salud, Escuela de Medicina, Departamento de Ciencias Básicas, Universidad Industrial de Santander, Bucaramanga, Colombia.
- Cesar M. Rueda Centro de Investigación de Enfermedades Tropicales (CINTROP), Facultad de Salud, Escuela de Medicina, Departamento de Ciencias Básicas, Universidad Industrial de Santander, Bucaramanga, Colombia.
- Fernando Martínez Centro de Investigaciones en Catálisis (CICAT), Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Colombia.
- Edgar Páez Centro de Investigaciones en Catálisis (CICAT), Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Colombia.
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2. Pinto, Juliana Guerra , Ferreira-Strixino, Juliana , Mittmann, Josane , Phototoxic effects of silicon bis (dimetilaminoetanoxi)-phthalocyanine (SiPc) on the viability ofLeishmania majorandLeishmania braziliensispromastigotes, 2016.
3. Prabhu C. P., Keshavananda , Nemakal, Manjunatha , Managa, Muthumuni , Nyokong, Tebello , Koodlur Sannegowda, Lokesh , Symmetrically Substituted Zn and Al Phthalocyanines and Polymers for Photodynamic Therapy Application, 2021.
4. Varzandeh, Mohammad , Mohammadinejad, Reza , Esmaeilzadeh-Salestani, Keyvan , Dehshahri, Ali , Zarrabi, Ali , Aghaei-Afshar, Abbas , Photodynamic therapy for leishmaniasis: Recent advances and future trends, 2021.
5. de Oliveira de Siqueira, Luciana Betzler , da Silva Cardoso, Verônica , Rodrigues, Igor Almeida , Vazquez-Villa, Ana Lúcia , dos Santos, Elisabete Pereira , da Costa Leal Ribeiro Guimarãe, Bruno , dos Santos Cerqueira Coutinho, Cristal , Vermelho, Alane Beatriz , Junior, Eduardo Ricci , Development and evaluation of zinc phthalocyanine nanoemulsions for use in photodynamic therapy forLeishmaniaspp., 2017.
6. Taylor, Viviana M. , Cedeño, David L. , Muñoz, Diana L. , Jones, Marjorie A. , Lash, Timothy D. , Young, Alexandra M. , Constantino, Manuel H. , Esposito, Nicholas , Vélez, Iván D. , Robledo, Sara M. , In Vitro and In Vivo Studies of the Utility of Dimethyl and Diethyl Carbaporphyrin Ketals in Treatment of Cutaneous Leishmaniasis , 2011.
7. Hernández, Indira Paola , Montanari, Jorge , Valdivieso, Wilfredo , Morilla, Maria Jose , Romero, Eder Lilia , Escobar, Patricia , In vitro phototoxicity of ultradeformable liposomes containing chloroaluminum phthalocyanine against New World Leishmania species, 2012.
8. Taylor, Viviana M. , Cedeño, David L. , Robledo, Sara M. , Fototerapia para el tratamiento de la leishmaniasis cutánea, 2011.
9. Silva, Emanoel Pedro de Oliveira , Mittmann, Josane , Ferreira, Vitória Tonini Porto , Cardoso, Maria Angélica Gargione , Beltrame, Milton , Photodynamic effects of zinc phthalocyanines on intracellular amastigotes of Leishmania amazonensis and Leishmania braziliensis , 2015.
10. Moghassemi, Saeid , Dadashzadeh, Arezoo , de Souza, Paulo Eduardo Narcizo , Azevedo, Ricardo Bentes , Amorim, Christiani A. , AlPc/ZnPc-based oncological photodynamic therapy for a selective eradication of leukemic cells from ovarian tissue, 2021.
11. Ribeiro, Jefferson Bruno Pereira , Miranda-Vilela, Ana Luisa , Graziani, Daniel , Gomes, Misléia Rodrigues de Aguiar , Amorim, Ana Angélica Santarem , Garcia, Rafaela Debastiani , de Souza Filho, José , Tedesco, Antônio Cláudio , Primo, Fernando Lucas , Moreira, Jonathan Rosa , Lima, Alexandre Vasconcelos , Sampaio, Raimunda Nonata Ribeiro , Evaluation of the efficacy of systemic miltefosine associated with photodynamic therapy with liposomal chloroaluminium phthalocyanine in the treatment of cutaneous leishmaniasis caused by Leishmania (L.) amazonensis in C57BL/6 mice, 2016.
12. Ozlem‐Caliskan, Sercin , Ertabaklar, Hatice , Bilgin, Mehmet Dincer , Ertug, Sema , Evaluation of photodynamic therapy against Leishmania tropica promastigotes using different photosensitizers, 2022.
13. Van Der Snoek, EM , Robinson, DJ , Van Hellemond, JJ , Neumann, HAM , A review of photodynamic therapy in cutaneous leishmaniasis, 2008.
14. Nadhman, Akhtar , Nazir, Samina , Ihsanullah Khan, Malik , Arooj, Syeda , Bakhtiar, Muhammad , Shahnaz, Gul , Yasinzai, Masoom , PEGylated silver doped zinc oxide nanoparticles as novel photosensitizers for photodynamic therapy against Leishmania, 2014.
15. Deda, Daiana K. , Iglesias, Bernardo A. , Alves, Eduardo , Araki, Koiti , Garcia, Celia R. S. , Porphyrin Derivative Nanoformulations for Therapy and Antiparasitic Agents, 2020.
16. Navasconi, Taisa Rocha , dos Reis, Vanessa Nesi , Freitas, Camila Fabiano , Pereira, Paulo Cesar de Souza , Caetano, Wilker , Hioka, Noboru , Lonardoni, Maria Valdrinez Campana , Aristides, Sandra Mara Alessi , Silveira, Thaís Gomes Verzignassi , Photodynamic Therapy With Bengal Rose and Derivatives Against Leishmania amazonensis, 2017.
17. Ozlem-Caliskan, Sercin , Ilikci-Sagkan, Rahsan , Karakas, Hatice , Sever, Sevgi , Yildirim, Cansu , Balikci, Misra , Ertabaklar, Hatice , Efficacy of malachite green mediated photodynamic therapy on treatment of Cutaneous Leishmaniasis: In vitro study, 2022.
18. Vital-Fujii, D.G. , Baptista, M.S. , Progress in the photodynamic therapy treatment of Leishmaniasis, 2021.
19. Lopez, Tessy , Ortiz, Ema , Alvarez, Mayra , Navarrete, Juan , Odriozola, Jose A. , Martinez-Ortega, Fernando , Páez-Mozo, Edgar A. , Escobar, Patricia , Espinoza, Karla A. , Rivero, Ignacio A. , Study of the stabilization of zinc phthalocyanine in sol-gel TiO2 for photodynamic therapy applications, 2010.
20. Dutta, Sujoy , Ongarora, Benson G. , Li, Hairong , Vicente, Maria da Graca H. , Kolli, Bala K. , Chang, Kwang Poo , Moreno, Silvia N. , Intracellular Targeting Specificity of Novel Phthalocyanines Assessed in a Host-Parasite Model for Developing Potential Photodynamic Medicine, 2011.
21. Lopera, A.A. , Velásquez, A.M.A. , Clementino, L.C. , Robledo, S. , Montoya, A. , de Freitas, L.M. , Bezzon, V.D.N. , Fontana, C.R. , Garcia, C. , Graminha, M.A.S. , Solution-combustion synthesis of doped TiO 2 compounds and its potential antileishmanial activity mediated by photodynamic therapy, 2018.
22. Ozlem Caliskan, Sercin , Ilıkcı Sagkan, Rahsan , Karakas, Hatice , Sever, Sevgi , Yildirim, Cansu , Balikci, Mısra , Ertabaklar, Hatice , Efficacy of Malachite Green Mediated Photodynamic Therapy on Treatment of Cutaneous Leishmaniasis: in vitro Study, 2022.
