Reproducibility of serological tests for the diagnosis of Trypanosoma cruzi infection in pregnant women in an endemic area of Santander, Colombia
Abstract
Introduction: The diagnosis of chronic Trypanosoma cruzi infection is supported by serological tests whose reproducibility has not been well documented.
Objective: To evaluate the reproducibility of the serological tests ELISA, IFAT and IHAT for the diagnosis of T. cruzi infection in pregnant women in an endemic zone in Santander.
Materials and methods: Through an evaluation study of diagnostic technologies, the reproducibility of the serological tests ELISA, IFAT and IHAT was determined in serum and eluted blood from pregnant women living in an endemic area for Chagas’ disease in Santander. The samples were selected by cross sectional sampling. The software Stata™ version 10.0 was used for statistical analysis. By means of the comparison of the highest kappa coefficient of each technique, the test with the best reproducibility was determined.
Results: A total of 777 samples were tested. In serum, ELISA (cutoff point: 0.3), IFAT (cutoff point: 1/32) and IHAT (cutoff point: 1/16) had kappa coefficients greater than 0.8 (0.98, 95% CI: 0.93-1.00; 0.98, 95% CI: 0.92-1.00 and 0.88, 95% CI: 0.74-0.97, respectively); no statistically significant differences among the three tests were found (p> 0.05). For the blood eluates, kappa coefficients were below 0.8 (highest kappa: 0.55, 95% CI: 0.41-0.68).
Conclusions: For the three serological tests using serum, the reproducibility determined by the kappa coefficient was perfect. Selecting any of them is useful for the diagnosis of T. cruzi infection. Given its simplicity and cost, the ELISA test is recommended for screening for this infection.
Downloads
Some similar items:
- Luz Adriana Botero, Ana María Mejía, Omar Triana, Biological and genetic characterization of two Colombian clones of Trypanosoma cruzi groups I and II , Biomedica: Vol. 27 No. 1esp (2007): Enfermedad de Chagas
- Patricia Escobar, Katherine Paola Luna, Indira Paola Hernández, César Mauricio Rueda, María Magdalena Zorro, Simon L. Croft, In vitro susceptibility of Trypanosoma cruzi strains from Santander, Colombia, to hexadecylphosphocholine (miltefosine), nifurtimox and benznidazole , Biomedica: Vol. 29 No. 3 (2009)
- María Clara Echeverry, Nubia Catalina Tovar, Guillermo Mora, Presence of antibodies to cardiac neuroreceptors in patients with Chagas disease , Biomedica: Vol. 29 No. 3 (2009)
- Sandra Paola Santander, Adriana Cuéllar, María del Carmen Thomas, Fanny Guzmán, Alberto Gómez, Manuel Carlos López, Concepción Puerta, Expression of markers on dendritic cells from chronic chagasic patients stimulated with the KMP-11 protein and the K1 peptide from Trypanosoma cruzi , Biomedica: Vol. 27 No. 1esp (2007): Enfermedad de Chagas
- Paula Ximena Pavía, Nubia Lucía Roa, Ana María Uribe, Concepción Judith Puerta, Using S35-S36 and TcH2AF-R primer-based PCR tests to follow-up a Chagas’ disease patient who had undergone a heart transplant , Biomedica: Vol. 31 No. 2 (2011)
- Richard Hoyos, Lisandro Pacheco, Luz Adriana Agudelo, German Zafra, Pedro Blanco, Omar Triana, Seroprevalence of Chagas disease and associated risk factors in a population of Morroa, Sucre , Biomedica: Vol. 27 No. 1esp (2007): Enfermedad de Chagas
- Dairo Alonso Rendón, Carlos M. Genes, Omar Triana, Myocardial cellular damage and the activity of the mitochondrial ATP synthase in rats infected with a Colombian strain of Trypanosoma cruzi , Biomedica: Vol. 27 No. 1esp (2007): Enfermedad de Chagas
- Concepción Judith Puerta, Johana María Guevara, Paula Ximena Pavía, Marleny Montilla, Rubén Santiago Nicholls, Edgar Parra, Yuli Katherine Barrera, Evaluation of TcH2AF-R and S35-S36 primers in PCR tests for the detection of Trypanosoma cruzi in mouse cardiac tissue , Biomedica: Vol. 28 No. 4 (2008)
- Rubén Santiago Nicholls, Zulma Milena Cucunubá, Angélica Knudson, Astrid Carolina Flórez, Marleny Montilla, Concepción Judith Puerta, Paula Ximena Pavía, Acute Chagas disease in Colombia: a rarely suspected disease. Report of 10 cases presented during the 2002-2005 period , Biomedica: Vol. 27 No. 1esp (2007): Enfermedad de Chagas
- Concepción Judith Puerta, Paula Ximena Pavia, Marleny Montilla, Carolina Flórez, Giomar Herrera, Juan Manuel Ospina, Fred Manrique, Rubén Santiago Nicholls, The first case of congenital Chagas’ disease analyzed by AP-PCR in Colombia , Biomedica: Vol. 29 No. 4 (2009)
Article metrics | |
---|---|
Abstract views | |
Galley vies | |
PDF Views | |
HTML views | |
Other views |