Concentración del virus Dengue 2 en la hemolinfa de mosquitos aedes aegypty inoculados intratoracicamente

Abstract

A study was made to determine if it was possible to demonstrate the replication of Dengue 2 virus in the haemolymph of Aedes aegypti mosquitoes previously inoculated intrathoracically. The results showed that the virus could he detected in the circulating haemolymph of the mosquitoes for several days after the inoculation. In a first experiment, in mosquitoes Aedes aegypti inoculated parenterally with approximately 50 PFU of dengue 2 virus, it was possible to demonstrate the virus in the haemolymph in 98% (89/91) of the mosquitoes examined between the third and the tenth day after inoculation. Between the 11th and the 14 th day the positiveness was 36% (9/25). The average virus titer in the haemolymph was dex 2.25 PFU per 0.3 ul. In a second experiment of 18 mosquitoes inoculated 28 days before virus was demonstrated in the haemolymph of 16 specimens. A simple method to obtain haemolymph from mosquitoes is described.