Cuantificación nefelométrica de proteínas plasmáticas Condiciones de estandarización

Abstract

An experimental procedure of standardization for quantitation of plasma proteins by immunochemical nephelometric technique is presented. Transferrin was quantitated in 39 sera from healthy  volunteers. It was found that optimal conditions included 10 minutes incubation time, room temperature of 30%. dilution of antitransferrin serum in saline buffer solution pH 7.4 eitb Polyethilen-Glycol al 4% concentration. It was found that under these conditions the normal Transferrin concentration of the tested population range from 186 to 406 mg/dl. result which es in agreement with other similar studies.