Construcción de un banco de clones de cDNA a partir de mRNA de P. falciparum

Abstract

Poly A mRNA was isolated from P. falciparum (Colombian strain FCB-2). The parasites were 34-36 hours old trophozoites. The messenger RNA was translated in a rabbit reticulocyte cell-free system and 35S-methionine labelled proteins were analized with S. D. S. PAGE. The translation products range were between 20 and 200 Kd and correlate closely with the in-vivo labelled protein, using stringent synchronous cultures. A cDNA library has been constructed in phage Agt10 from poly A mRNA. Cloning efficency was 4.4x10 6 recombinant per ug cDNA.