Análisis de plásmidos recombinantes con insertos de ADN genómico de Plasmodium falciparum

Abstract

We describe here the growth and preparation of pUC18 recombinant plasmids from a Plasmodium falciparum genomic DNA library. The protocol used is a modification of alkaline lysis method (1). This method yields a quantity of plasmid DNA that is suficient for subsequent analysis by restriction digestion and agarose electrophoresis.