El virus de la D.V.B. como agente contaminante en cultivo de tejidos animales

Abstract

The Hovine Viral Diarrhoea Virus (BWV)belongs to the genus Pestisvirus. It is the cause or reproductive disorders in catlle and is widespread in the conuntry. There are noncytophatogenic strains of the BVDV very difficult to detect inthe primary tissue culture and cell lines, ordinarilly used in diagnostic test or research activities. Foetal calf serum (FCS) is the common source of contamulation for the animal tissue culture, because of the poor quality controll during its collection and markenting under local conditions. It is possibly to fmd two kind of situations related to BVDV and the presence of viral particles; in each case is possible to have problems with the laboratory procedures. In order to avoid BWV tissue culture contamination, in our laboratory it has been used an indirect inmunofluorescense tedinique for the detectionofthe Wusinfoetalcaüserumandtissue cultures, havingresults asfoilows: 28.5%positives from 32 FCS samples from differnt sources and 42.8% from tissue cuitures from different animal origen and several research centres. Come recomdations are made for the prevention and control ot this problem at diagnostic and researchlaboratorylevelliietheuse of BI, indirect peroxidase techniques, or inmunopresitation for the detection of the Wus, and the use of a few number of fwtuses for each lot of FCS in order to reduce the risk of contamination from this source.