Evaluación del efecto del ácido nalidíxico, ampicilina, kanamicina, penicilina G y polimixina B en los cultivos de promastigotes de leishmania

Abstract

The effect of several antibiotics (nalidixic acid, ampicillin, kanamycin, penicillin G and polimyxin B) concentrations was evaluated on promastigote growth of Leishmania braziliensis braziliensis, Leishmania danovani chagasi, and Leishmania mexicana amazonensis which had been maintenance in vitro. Penicillin G and ampicillin could be added until concentrations of 1000 ug/ml and 500 ug/ml, respectively, without any parasite alteration. Promastigote populations of all strains decreased after the addition of polimqxin B; consequently, this antibiotic is not recommended or useful in Leishmania cultures. Nalidixic acid and kanamycinmay be added to Leishmania cultures, considering the particular Leishmania strain and the antimicrobial concentration chosen for it.