Construcción de una genoteca de Plasmodium falciparum en el fago lambda EMBL-4 y aislamiento de clonos que contienen la secuencia repetitiva de 692 pares de bases (PFCOL692)

Abstract

A Plasmodium falciparum genomic library was constructed in the vector EMBL-4 Lambda phage. It was screened with REP 692 a Plasmodium falciparum repetitive fragment which was previously cloned in plasmid pUC18. Twenty eight clones were isolated. The methodology both for construction of the library and for isolation of clones are described, and the importance of the library for further studies of the parasite's DNA  structure is discussed. Keywords: Plasmodium, repetitive DNA , PFCOL692.