Determinación de la prevalencia de cisticercosis porcina en cuatro veredas del municipio de Coyaima

Abstract

One hundred and seventeen sera of alive pigs in rural areas from the counties of Totarco Dinde, Totarco Piedras, Doyare La Esmeralda and Lomas de Guaguarco, belonging to the municipality of Coyaima in the department of Tolima, were obtained during the period from June to December in 1992 and analyzed for antibodies against larvae of Taenia solium by enzyme linked immunosorbent assay (ELISA) in order to establish the prevalence of cysticercosis in pigs. The ELISA test for immunodiagnosis of cysticercosis in pigs was standardized. The optimum concentration of total delipidized antigen of T. solium larvae was 5 ugr/ml and the optimum dilutions of serum and conjugate (anti-pig IgG linked to alkaline phosphatase) were 1:400 and 1:3000 respectively. The parameters of the test were : sensitivity: 100%, specificity: 97.14%, positive predictive value: 90% and negative predictive value: 100%, using serum samples from healthy pigs and swines having a parasitologically confirmed diagnosis of cysticercosis. Prevalence of cysticercosis in pigs from the municipality of Coyaima was 25,64%, being 37,5%, 33,33%, 17,39% and 15,63% for the counties of Doyare La Esmeralda, Totarco Piedras, Totarco Dinde and Lomas de Guaguarco, respectively. The ELlSA test carried out in this survey might be applied for both immunodiagnosis of cysticercocis in pigs and seroepidemiological surveys of prevalence of the disease in swines in order to set up control measures against this parasite.