Diseño y evaluación de un sistema inmunoenzimático rápido de tercera generación para la detección de anticuerpos antiVlH

Abstract

This paper describes the development of a third generation method for the detection of specific antibodies against the Human lmmunodeficiency Virus (VIH) types 1 and 2. The method uses a Microelisa, based on the use of a mixture of synthetic peptides as antigens derived from gp41 and p24 of HIV 1 and p36 of HIV 2. This system requires a total of twenty minutes (20') in order to obtain a result with 100% specificity and 99% sensitivity, comparable to the new third - generation commercially available systems.