Evidence of the circulation of hepatitis A virus, subgenotype IA, in environmental samples from Antioquia, Colombia
Abstract
Introduction: Hepatitis A virus (HAV) is an important pathogen, typically transmitted via the faecal-oral route. The epidemiology of the infection is directly related to drinking water access and adequate disposal of sewage water. Objective: To determine the presence and identify the genotype of HAV in environmental samples from eight municipalities and two villages in Antioquia, northwestern Colombia. Materials and methods: Three serial samplings were done between December, 2012, and April, 2014. Water samples were obtained from drinking water plants prior to treatment, as well as from the main reserve of wastewater in each municipality included in the study. Viral concentrations for the two types of sample sources were determined by filtration/tangential ultrafiltration and polyethyleneglycol plus flocculation with skimmed milk, respectively. Total ARN was subsequently obtained from each sample and the VP3-VP1 region amplified for detection of the viral genome. The genotype was determined by amplification of the VP1-2B region. Results: The HAV genome was detected in samples from drinking water plants at Puerto Berrío, Frontino and Nutibara, and in wastewater samples from the municipalities of Arboletes, Zaragoza and Venecia. HAV subgenotype IA was identified using phylogenetic analysis. Conclusion: In this study, HAV was identified in 6.6% of the samples from drinking water plants and 13.3% of wastewater samples. This is the first report of HAV subgenotype IA circulating in environmental samples from Antioquia.
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Knipe D, Howley P. Fields Virology. Sixth edition. Philadelphia: Lippincott Williams & Wilkins; 2013. p. 551-81.
Provost PJ, Hilleman MR. Propagation of human hepatitis A virus in cell culture in vitro. Exp Biol Med. 1979;160:213-21. http://dx.doi.org/10.3181/00379727-160-40422
Feng Z, Hensley L, McKnight KL, Hu F, Madden V, Ping L, et al. A pathogenic picornavirus acquires an envelope by hijacking cellular membranes. Nature. 2013;496:367-71. http://dx.doi.org/10.1038/nature12029
Robertson BH, Jansen RW, Khanna B, Totsuka A, Nainan OV, Siegl G, et al. Genetic relatedness of hepatitis A virus strains recovered from different geographical regions. J Gen Virol. 1992;73:1365-77. http://dx.doi.org/10. 1099/0022-1317-73-6-1365
Cristina J, Costa-Mattioli M. Genetic variability and molecular evolution of hepatitis A virus. Virus Res. 2007; 127:151-7. http://dx.doi.org/10.1016/j.virusres.2007.01.005
Aguirre S, Malirat V, Scodeller E, Mattion N. First full-length genomic sequence of a hepatitis A virus isolated in Argentina shows recombination between subgenotypes IA and IB. Virus Res. 2011;155:316-24. http://dx.doi.org/10.1016/j.virusres.2010.10.030
Joshi MS, Walimbe AM, Chitambar SD. Evaluation of genomic regions of hepatitis A virus for phylogenetic analysis: Suitability of the 2C region for genotyping. J Virol Methods. 2008;153:36-42. http://dx.doi.org/10.1016/j.jviromet.2008.06.014
Báez-Triana PA, Navas-Navas MC. Infección por el virus de la hepatitis A: epidemiología y diversidad génica. Iatreia. 2015;28:157-69. http://dx.doi.org/10.17533/udea.iatreia.v28n2a06
Nainan OV, Xia G, Vaughan G, Margolis HS. Diagnosis of hepatitis A virus infection: A molecular approach. Clin Microbiol Rev. 2006;19:63-79. http://dx.doi.org/10.1128/cmr.19.1.63-79.2006
Theamboonlers A, Abe K, Thongmee C, Poovorawan Y. Complete coding sequence and molecular analysis of hepatitis A virus from a chimpanzee with fulminant hepatitis. J Med Primatol. 2012;41:11-7. http://dx.doi.org/10.1111/j.1600-0684.2011.00515.x
Deinhardt F, Holmes AW, Capps RB, Popper H. Studies on the transmission of human viral hepatitis to marmoset monkeys. I. Transmission of disease, serial passages, and description of liver lesions. J Exp Med. 1967;125:673-88. http://dx.doi.org/10.1084/jem.125.4.673
Sulbarán Y, Gutiérrez CR, Márquez B, Rojas D, Sánchez D, Navas J, et al. Hepatitis A virus genetic diversity in Venezuela: Exclusive circulation of subgenotype IA and evidence of quasispecies distribution in the isolates. J Med Virol. 2010;82:1829-34. http://dx.doi.org/10.1002/jmv.21856
Prado T, Fumian TM, Miagostovich MP, Gaspar AM. Monitoring the hepatitis A virus in urban wastewater from Rio de Janeiro, Brazil. Trans R Soc Trop Med Hyg. 2012; 106:104-9. http://dx.doi.org/10.1016/j.trstmh.2011.10.005
Betancourt WQ, Querales L, Sulbarán YF, Rodríguez-Díaz J, Caraballo L, Pujol FH. Molecular characterization of sewage-borne pathogens and detection of sewage markers in an urban stream in Caracas, Venezuela. Appl Environ Microbiol. 2010;76:2023-6. http://dx.doi.org/10.1128/aem. 02752-09
Yáñez LA, Lucero NS, Barril PA, Díaz M de P, Tenaglia MM, Spinsanti LI, et al. Evidence of hepatitis A virus circulation in central Argentina: Seroprevalence and environmental surveillance. J Clin Virol. 2014;59:38-43. http://dx.doi.org/10.1016/j.jcv.2013.11.005
Sánchez J, Villamil L-T, Rodríguez J, Rincón M, Méndez J, Sánchez C, et al. Genotipos del virus de hepatitis A circulantes en Colombia, 2008-2012. Hechos Microbiológicos. 2013;4:94.
Cuthbert JA. Hepatitis A: Old and new. Clin Microbiol Rev. 2001;14:38-58. http://dx.doi.org/10.1128/cmr.14.1.38-58.2001
Organización Mundial de la Salud. Hepatitis A. Nota descriptiva N.°328. Fecha de consulta: 1 de junio de 2015. Disponible en: http://www.who.int/mediacentre/factsheets/fs328/es/
Franco E, Meleleo C, Serino L, Sorbara D, Zaratti L. Hepatitis A: Epidemiology and prevention in developing countries. World J Hepatol. 2012;4:68-73. http://dx.doi.org/10.4254/wjh.v4.i3.68
Havelaar AH, Kirk MD, Torgerson PR, Gibb HJ, Hald T, Lake RJ, et al. World Health Organization global estimates and regional comparisons of the burden of foodborne disease in 2010. PLoS Med. 2015;12:e1001923. http://dx. doi.org/10.1371/journal.pmed.1001923
Jacobsen KH, Wiersma ST. Hepatitis A virus sero-prevalence by age and world region, 1990 and 2005. Vaccine. 2010;28:6653-7. http://dx.doi.org/10.1016/j.vaccine. 2010.08.037
Vacchino MN. Incidence of hepatitis A in Argentina after vaccination. J Viral Hepat. 2008;15:47-50. http://dx.doi.org/10.1111/j.1365-2893.2008.01029.x
Vizzotti C, González J, Gentile A, Rearte A, Ramonet M, Cañero-Velasco MC, et al. Impact of the single-dose immunization strategy against hepatitis A in Argentina. Pediatr Infect Dis J. 2014;33:84-8. http://dx.doi.org/10.1097/inf.0000000000000042
Ximenes RA, Martelli CM, Amaku M, Sartori AM, de Soárez PC, Novaes HM, et al. Modelling the force of infection for hepatitis A in an urban population-based survey: A comparison of transmission patterns in Brazilian macro-regions. PLoS ONE. 2014;9:e94622. http://dx.doi.org/10.1371%2Fjournal.pone.0094622
Ministerio de Salud y Protección Social. Lineamiento estratégico para la introducción de la vacuna contra la hepatitis A en el esquema del Programa Ampliado de Inmunizaciones – PAI. Colombia 2013. Fecha de consulta: 13 de julio de 2015. Disponible en: https://www.minsalud.gov.co/Documentos%20y%20Publicaciones/Lineamientos%20estrat%C3%A9gicos%20para%20la%20Introducci%C3%B3n%20de%20la%20vacuna%20contra%20la%20Hepatitis%20A.pdf
Instituto Nacional de Salud. Informe del evento hepatitis A hasta el periodo epidemiológico XIII, Colombia, 2014. Fecha de consulta: 15 de junio de 2015. Disponible en: http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/Informe%20de%20Evento%20Epidemiolgico/Hepatitis%20A%202014.pdf
Instituto Nacional de Salud. Informe del evento hepatitis A hasta el periodo epidemiológico XII, Colombia, 2015. Fecha de consulta: 10 de febrero de 2016. Disponible en: http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/Informe%20de%20Evento%20Epidemiolgico/HEPATITIS%20A%20Periodo%20XII%202015.pdf
Instituto Nacional de Salud. Vigilancia rutinaria por evento departamental 2014. Fecha de consulta: 10 de febrero de 2016. Disponible en: http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/sivigila/Paginas/vigilancia-rutinaria.aspx
Peláez-Carvajal D, Rodríguez J-A, Rocha-Hernández L, Ardila-Buitrago M, Méndez JA, Sánchez CM. Detección de virus entéricos en agua para consumo humano asociados a brotes de enfermedad diarreica aguda y hepatitis A en Colombia, 2008-2013. Hechos Microbiológicos. 2013;4:80.
Dirección Seccional de Salud de Antioquia. Casos e incidencia de hepatitis A por subregión y municipio de Antioquia 2000-2012. Otras enfermedades en la región 2000 a 2012. Fecha de consulta: 20 de marzo de 2015. Disponible en: http://www.dssa.gov.co/index.php/estadisticas/eventos-en-salud-publica?start=4
Secretaría de Salud y Protección Social de Frontino. Casos presentados de hepatitis A en el municipio de Frontino durante los años 2011, 2012 y 2013. Frontino, Antioquia: Secretaría de Salud y Protección Social; 2014.
Secretaría Seccional de Salud y Protección Social de Antioquia. Población total según zona por subregiones y municipios. Antioquia 2009. Fecha de consulta: 2 de julio de 2015. Disponible en: https://www.dssa.gov.co/index.php/estadisticas/poblacion/item/66-poblacion-2009
Peláez D, Rodríguez JA, Rocha EL, Rey GJ. Estandariza-ción de un método de concentración y detección de virus entéricos en aguas de consumo. Biomédica. 2010;30:276-86. http://dx.doi.org/10.7705/biomedica.v30i2.191
Sobsey MD, Oglesbee SE, Wait DA. Evaluation of methods for concentrating hepatitis A virus from drinking water. Appl Environ Microbiol. 1985;50:1457-63.
González MM, Sarmiento L, Castaño JC, Giraldo AM. Detección de poliovirus en aguas residuales de Armenia, Colombia. Rev Salud Pública (Bogotá). 2006;8(Supl.1):13-26. http://dx.doi.org/10.1590/S0124-00642006000400002
Martínez-Wassaf MG, Pisano MB, Barril PA, Elbarcha OC, Pinto MA, Mendes de Oliveira J, et al. First detection of hepatitis E virus in Central Argentina: Environmental and serological survey. J Clin Virol. 2014;61:334-9. http://dx.doi.org/10.1016/j.jcv.2014.08.016
Calgua B, Mengewein A, Grunert A, Bofill-Mas S, Clemente-Casares P, Hundesa A, et al. Development and application of a one-step low cost procedure to concentrate viruses from seawater samples. J Virol Methods. 2008;153: 79-83. http://dx.doi.org/10.1016/j.jviromet.2008.08.003
Calgua B, Rodríguez-Manzano J, Hundesa A, Suñen E, Calvo M, Bofill-Mas S, et al. New methods for the concentration of viruses from urban sewage using quantitative PCR. J Virol Methods. 2013;187:215-21. http://dx.doi.org/10.1016/j.jviromet.2012.10.012
Schwab KJ, De León R, Sobsey MD. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Appl Environ Microbiol. 1995;61:531-7.
Mitsui T, Tsukamoto Y, Hirose A, Suzuki S, Yamazaki C, Masuko K, et al. Distinct changing profiles of hepatitis A and E virus infection among patients with acute hepatitis, patients on maintenance hemodialysis and healthy indivi-duals in Japan. J Med Virol. 2006;78:1015-24. http://dx.doi.org/10.1002/jmv.20657
Ministerio de la Protección Social. Decreto 1575 de 2007. Fecha de consulta: 10 de febrero de 2016. Disponible en: https://www.minambiente.gov.co/images/normativa/decretos/ 2007/dec_1775_2007.pdf
Ministerio de Protección Social, Ministerio de Ambiente, Vivienda y Desarrollo Territorial. Resolución 2115 de 2007. Fecha de consulta: 10 de febrero de 2016. Disponible en: http://www.ins.gov.co/tramites-y-servicios/programas-de-calidad/Documents/resolucion%202115%20de%202007,MPS-MAVDT.pdf
Instituto Nacional de Salud. Estado de la vigilancia de la calidad del agua para consumo humano en Colombia-2013. Fecha de consulta: 12 de junio de 2015. Disponible en: http://www.ins.gov.co/sivicap/Normatividad/2014%20Estado%20de%20la%20vigilancia%20de%20la%20calidad%20del%20agua%202013.pdf?Mobile=1&Source=%2Fsivicap%2F_layouts%2Fmobile%2Fview.aspx%3FList%3Ddc462e4b-5de8-4a2f-be3a-08ad1c837db7%26View%3D0ac5f5c5-4988-442d-bc0e-2c07af4f66a5%26CurrentPage%3D1
Albinana-Giménez N, Clemente-Casares P, Bofill-Mas S, Hundesa A, Ribas F, Girones R. Distribution of human polyoma viruses, adenoviruses, and hepatitis E virus in the environment and in a drinking-water treatment plant. Environ Sci Technol. 2006;40:7416-22. http://dx.doi.org/10. 1021/es060343i
Girones R, Carratalà A, Calgua B, Calvo M, Rodríguez-Manzano J, Emerson S. Chlorine inactivation of hepatitis E virus and human adenovirus 2 in water. J Water Health. 2014;12:436-42. http://dx.doi.org/10.2166/wh.2014.027
Widenmann A, Fischer B, Straub U, Wang C, Flehmig B, Schoenen D. Disinfection of hepatitis A virus and MS-2 coliphage in water by ultraviolet irradiation: Comparison of UV-susceptibility. Water Science and Technology. 1993;27: 335-8.
Villar LM, de Paula VS, Diniz-Mendes L, Lampe E, Gaspar AMC. Evaluation of methods used to concentrate and detect hepatitis A virus in water samples. J Virol Methods. 2006;137:169-76. http://dx.doi.org/10.1016/j.jviromet.2006.06.008
Chigor V, Okoh A. Quantitative RT-PCR detection of hepatitis A virus, rotaviruses and enteroviruses in the Buffalo River and source water dams in the Eastern Cape Province of South Africa. Int J Environ Res Public Health. 2012;9:4017-32. http://dx.doi.org/10.3390/ijerph9114017
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