Modulation of the norfloxacin resistance in Staphylococcus aureus by Croton campestris A. and Ocimum gratissimum L.
Keywords:
Croton campestres A., Ocimum gratissimum L., modificación de la resistencia, antibióticos, Sistema de eflujo de NorA, Staphylococcus aureus.
Abstract
Introduction: Some species of Staphylococcus are often recognized as etiological agents of many animal and human opportunistic infections. This study is the first test of change in resistance of
antibiotic activity by Croton campestris A. and Ocimum gratissimum L. against multiresistant strains of Staphylococcus aureus.
Objective: In this study, the hexane and methanol extract of Croton campestris A. and Ocimum gratissimum L. was tested for antibacterial activity alone and in combination with norfloxacin against the strain SA1199B.
Materials and methods: The minimum inhibitory concentration (MIC) and the modulatory effect of extracts was assayed using microtitre assay.
Results: By the fact of the MIC observed was not clinically relevant (MIC= 512 to ≥1.024 μg/ml), the antibiotic activity of norfloxacin was enhanced when this antibiotic was combined with sub-inhibitory concentrations of extracts, mainly the hexane extracts.
Conclusions: These results indicate that the assayed extracts present compounds that can be used as a putative efflux pump inhibitor, indicating that Croton campestris A. and Ocimum gratissimum L. can be a source of plant derived products with antibiotic modifier activity.
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References
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24. Ferreira FS, Brito SV, Costa JG, Alves RR, Coutinho HD, Almeida WO. Is the body fat of the lizard Tupinambis merianae effective against bacterial infections? J Ethnopharmacol. 2009;126:233-7.
25. Nicolson K, Evans G, O´Toole PW. Potentiation of methicillin activity against methicillin-resistant Staphylococcus aureus by diterpenes. FEMS Microbiol Lett. 1999;179:233-9.
26. Juven J, Kanner J, Schved F, Weisslowicz H. Factors that interact with antimicrobial action of thyme essential oil and its active constituents. J Appl Bacteriol. 1994;76:626-31.
27. Burt S. Essential oils: their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol 2004;94:223-53.
28. Helander IM, Alakomi HL, Latva-Kala K, Sandholm TM, Pol I, Smid EJ, et al. Characterization of the action of selected essential oil components on Gram-negative bacteria. J Agricult Food Chem. 1998;46:3590-5.
29. Wendakoon C, Sakaguchi M. Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices. J Food Protect. 1995;58:280-3.
30. Wagner H, Ulrich-Merzenich G. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine 2009;16:97-110.
2. Nostro A, Blanco AR, Cannatelli MA, Enea V, Flamini G, Morelli I. Susceptibility of methicillin-resistant staphylococci to oregano essential oil, carvacrol and thymol. FEMS Microbiol Lett. 2004;230:191-5.
3. Verhoeff JD, Beaujean DH, Vlok HA, Baars AA, Meyler A, Werkwn VD. A dutch approach to methicillin-resistance Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 1999;18:461-6.
4. Coutinho HD, Costa JG, Lima EO, Falcão-Silva VS, Siqueira-Júnior JP. Enhancement of the antibiotic activity against a multiresistant Escherichia coli by Mentha arvensis L. and chlorpromazine. Chemotherapy. 2008;54:328-30.
5. Coutinho HD, Costa JGM, Siqueira-Júnior JP, Lima EO. In vitro anti- staphylococcal activity of Hyptis martiusii Benth against methicillin-resistant Staphylococcus aureus-MRSA strains. Braz J Pharmacogn. 2008;18(Suppl.):670-5.
6. Oliveira FQ, Gobira BC, Guimarães CJ, Batista JM, Barreto M, Souza M. Plants species indicated in odontology. Braz J Pharmacogn. 2007;17:466-76.
7. Saúde-Guimarães DA, Faria AR. Natural compounds with anti-Trypanossoma cruzi activity. Braz J Pharmacogn. 2007;17:455-65.
8. Daferera DJ, Ziogas BN, Polissiou MG. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. Michiganensis. Crop Protect. 2003;22:39-44.
9. de Heluani CS, Catalán CA, Hernández LR, Burgueño-Tapia E, Joseph-Nathan P. Three new diterpenoids based on novel sarcopetalene skeleton from Croton sarcopetalus. J Nat Prod. 2000;63:222-5.
10. Cruz GL. Dicionário das plantas úteis do Brasil. 2ª ed. Rio de Janeiro: Ed. EDEL; 1982.
11. Lorenzi H, Matos FJ. Plantas medicinais do Brasil: nativas e exóticas. Nova Odessa: Instituto Plantarum de Estudos da Flora; 2002.
12. Martins JR, Alvarenga AA. Leaf Anatomy of alfavacacravo plants cultivated under colored nets. Ciênc Rural. 2008;39:82-7.
13. Matasyoh LG, Matasyoh JC, Wachira FN, Kinyua MG, Muigai AW, Mukiama TK. Chemical composition and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. Afr J Biotechnol. 2007;6:760-5.
14. Ueda-Nakamura T, Mendonça-Filho RR, Morgado-Díaz JA, Korehisa MP, Prado DF, Cortez AGD, et al. Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum. Parasitol Int. 2006;55:99-105.
15. Kaatz GW, Seo SM, Ruble CA. Efflux-mediated fluoroquinolone resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1993;37:1086-94.
16. Kaatz GW, Seo SM. Inducible NorA-mediated multidrug resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1995;39:2650-5.
17. Matos FJ. Introdução à Fitoquímica Experimental. 2ª Ed. Fortaleza: Edições UFC; 1997.
18. Javadpour MM, Juban MM, Lo WC, Bishop SM, Alberty JB, Cowell SM, et al. De novo antimicrobial peptides with low mammalian cell toxicity. J Med Chem. 1996;39:3107-13.
19. Stavri M, Piddock LJ, Gibbons S. Bacterial efflux pump inhibitors from natural sources. J Antimicrob Chemother. 2007;59:1247-60.
20. Senatore F, Rigano D, Formisano C, Grassia A, Basile A, Sorbo S. Phytogrowth-inhibitory and antibacterial activity of Verbascum sinuatum. Fitoterapia 2007;78:244-7.
21. Gibbons S. Anti-staphylococcal plant natural products. Nat Prod Rep. 2004;21:263-77.
22. Coutinho HD, Costa JG, Lima EO, Siqueira-Júnior JP. Additive effects of Hyptis martiusii Benth with aminoglycosides against Escherichia coli. Indian J Med Res. 2010;131:106-8.
23. Rodrigues FF, Costa JG, Coutinho HD. Synergy effects of the antibiotics gentamicin and the essential oil of Croton zehntneri. Phytomedicine. 2009;16:1052-5.
24. Ferreira FS, Brito SV, Costa JG, Alves RR, Coutinho HD, Almeida WO. Is the body fat of the lizard Tupinambis merianae effective against bacterial infections? J Ethnopharmacol. 2009;126:233-7.
25. Nicolson K, Evans G, O´Toole PW. Potentiation of methicillin activity against methicillin-resistant Staphylococcus aureus by diterpenes. FEMS Microbiol Lett. 1999;179:233-9.
26. Juven J, Kanner J, Schved F, Weisslowicz H. Factors that interact with antimicrobial action of thyme essential oil and its active constituents. J Appl Bacteriol. 1994;76:626-31.
27. Burt S. Essential oils: their antibacterial properties and potential applications in foods - a review. Int J Food Microbiol 2004;94:223-53.
28. Helander IM, Alakomi HL, Latva-Kala K, Sandholm TM, Pol I, Smid EJ, et al. Characterization of the action of selected essential oil components on Gram-negative bacteria. J Agricult Food Chem. 1998;46:3590-5.
29. Wendakoon C, Sakaguchi M. Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices. J Food Protect. 1995;58:280-3.
30. Wagner H, Ulrich-Merzenich G. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine 2009;16:97-110.
How to Cite
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Coutinho HD, Matias EF, Santos KK, Santos FA, Morais-Braga MFB, Souza TM, et al. Modulation of the norfloxacin resistance in Staphylococcus aureus by Croton campestris A. and Ocimum gratissimum L. biomedica [Internet]. 2011 Jun. 30 [cited 2024 May 16];31(4):608-12. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/409
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