Report on the First Colombian Consensus Conference for Flow Cytometric Immunophenotyping of hematological disorders

Carlos Saavedra, Sandra Milena Quijano, Martha Romero, Roberto Jaramillo, Rocío Orduz, Carolina Echeverri, Mónica Londoño, Alberto Orfao, .

Abstract

During July 7th and 8th of 2008, the First Colombian Consensus Conference for the Immunophenotyping of Hematological Malignancies was carried out in Cali, Colombia, with representatives from Colombia, Argentina, Chile, Perú and México. The consensus included professionals from the laboratory field and clinicians from different institutions that routinely operate clinical flow cytometry with an emphasis on lymphoma and leukemia immunophenotyping.In this consensus medical indications for flow cytometry use in the detection of hematological malignancies, technical considerations for staining and samples preparation protocols, evaluation of diagnostic immunophenotyping panels, data acquisition and interpretation strategies were analyzed, and, finally, how to report the results.

Downloads

Download data is not yet available.
  • Carlos Saavedra Departamento de Patología y Laboratorios, Hospital Universitario Fundación Santa Fe de Bogotá, Bogotá, D.C., Colombia
  • Sandra Milena Quijano Departamento de Microbiología, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia
  • Martha Romero Departamento de Patología y Laboratorios, Hospital Universitario Fundación Santa Fe de Bogotá, Bogotá, D.C., Colombia
  • Roberto Jaramillo Fundación Valle de Lili, Cali, Colombia
  • Rocío Orduz Clínica Colsanitas, S. A., Bogotá, D.C., Colombia
  • Carolina Echeverri Hospital Pablo Tobón Uribe, Medellín, Colombia
  • Mónica Londoño
  • Alberto Orfao Centro de Investigación del Cáncer (IBMCC-CSIC/USAL), Universidad de Salamanca, Salamanca, España

References

Ruiz-Arguelles A, Rivadeneyra-Espinoza L, Duque RE, Orfao A. Report on the Second Latin American Consensus Conference for flow cytometric immunophenotyping of hematological malignancies. Cytometry B. 2006;70:39-44.

Ruiz-Arguelles A, Duque RE, Orfao A. Report on the First Latin American Consensus Conference for Flow Cytometric Immunophenotyping of Leukemia. Cytometry. 1998;34:39-42.

Pui CH, Robison LL, Look AT. Acute lymphoblastic leukaemia. Lancet. 2008;371:1030-43.

Bene MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao A, et al. Proposals for the immunological classification of acute leukemias. Leukemia. 1995;9:1783-6.

Lucio P, Gaipa G, van Lochem EG, van Wering ER, Porwit-MacDonald A, Faria T, et al. BIOMED-I concerted action report: flow cytometric immunophenotyping of precursor B-ALL with standardized triple-stainings. BIOMED-1 concerted action investigation of minimal residual disease in acute leukemia: international standardization and clinical evaluation. Leukemia. 2001;15:1185-92.

Wetzler M, Dodge RK, Mrozek K, Stewart CC, Carroll AJ, Tantravahi R, et al. Additional cytogenetic abnormalities in adults with Philadelphia chromosome-positive acute lymphoblastic leukaemia: a study of the Cancer and Leukaemia Group B. Br J Haematol. 2004;124:275-88.

Hrusak O, Porwit-MacDonald A. Antigen expression patterns reflecting genotype of acute leukemias. Leukemia. 2002;16:1233-58.

Mori T, Sugita K, Suzuki T, Okazaki T, Manabe A, Hosoya R, et al. A novel monoclonal antibody, KOR-SA3544 which reacts to Philadelphia chromosome-positive acute lymphoblastic leukemia cells with high sensitivity. Leukemia. 1995;9:1233-9.

Tabernero MD, Bortoluci AM, Alaejos I, López-Berges MC, Rasillo A, Garcia-Sanz R, et al. Adult precursor B-ALL with BCR/ABL gene rearrangements displays a unique immunophenotype based on the pattern of CD10, CD34, CD13 and CD38 expression. Leukemia. 2001;15:406-14.

Uckun FM, Downing JR, Gunther R, Chelstrom LM, Finnegan D, Land VJ, et al. Human t(1;19)(q23;p13) pre-B acute lymphoblastic leukemia in mice with severe combined immunodeficiency. Blood. 1993;81:3052-62.

De Zen L, Orfao A, Cazzaniga G, Masiero L, Cocito MG, Spinelli M, et al. Quantitative multiparametric immunophenotyping in acute lymphoblastic leukemia: correlation with specific genotype. I. ETV6/AML1 ALLs identification. Leukemia. 2000;14:1225-31.

Weerkamp F, Dekking E, Ng YY, van der Velden, V, Wai H, Bottcher S, et al. Flow cytometric immunobead assay for the detection of BCR-ABL fusion proteins in leukemia patients. Leukemia. 2009;23:1106-17.

Pui CH, Campana D, Evans WE. Childhood acute lymphoblastic leukaemia –current status and future perspectives. Lancet Oncol. 2001;2:597-607.

Pui CH, Relling MV, Downing JR. Acute lymphoblastic leukemia. N Engl J Med. 2004;350:1535-48.

Del Canizo MC, Fernández ME, López A, Vidriales B, Villaron E, Arroyo JL, et al. Immunophenotypic analysis of myelodysplastic syndromes. Haematologica. 2003;88:402-7.

Orfao A, Ortuno F, de Santiago M, López A, San MJ. Immunophenotyping of acute leukemias and myelodysplastic syndromes. Cytometry A. 2004;58:62-71.

San Miguel JF, Vidriales MB, López-Berges C, Díaz-Mediavilla J, Gutiérrez N, Canizo C, et al. Early immunophenotypical evaluation of minimal residual disease in acute myeloid leukemia identifies different patient risk groups and may contribute to postinduction treatment stratification. Blood. 2001;98:1746-51.

Costa ES, Arroyo ME, Pedreira CE, García-Marcos MA, Tabernero MD, Almeida J, et al. A new automated flow cytometry data analysis approach for the diagnostic screening of neoplastic B-cell disorders in peripheral blood samples with absolute lymphocytosis. Leukemia. 2006;20:1221-30.

Sayala HA, Rawstron AC, Hillmen P. Minimal residual disease assessment in chronic lymphocytic leukaemia. Best Pract Res Clin Haematol. 2007;20:499-512.

Rawstron AC, Villamor N, Ritgen M, Bottcher S, Ghia P, Zehnder JL, et al. International standardized approach for flow cytometric residual disease monitoring in chronic lymphocytic leukaemia. Leukemia. 2007;21:956-64.

Nieto WG, Almeida J, Romero A, Teodosio C, López A, Henriques AF, et al. Increased frequency (12%) of circulating chronic lymphocytic leukemia-like B-cell clones in healthy subjects using a highly sensitive multicolor flow cytometry approach. Blood. 2009;114:33-7.

Quijano S, López A, Manuel SJ, Panizo C, Deben G, Castilla C, et al. Identification of leptomeningeal disease in aggressive B-cell non-Hodgkin’s lymphoma: improved sensitivity of flow cytometry. J Clin Oncol. 2009;27:1462-1469.

Fromm JR, Kussick SJ, Wood BL. Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Clin Pathol. 2006;126:764-80.

Palumbo GA, Parrinello N, Fargione G, Cardillo K, Chiarenza A, Berretta S, et al. CD200 expression may help in differential diagnosis between mantle cell lymphoma and B-cell chronic lymphocytic leukemia. Leuk Res. 2009;33:1212-6.

Lima M, Almeida J, Santos AH, dos Anjos TM, Alguero MC, Queiros ML, et al. Immunophenotypic analysis of the TCR-Vbeta repertoire in 98 persistent expansions of CD3(+)/TCR-alpha-beta(+) large granular lymphocytes: utility in assessing clonality and insights into the pathogenesis of the disease. Am J Pathol. 2001;159:1861-8.

San Miguel JF, Gutiérrez NC, Mateo G, Orfao A. Conventional diagnostics in multiple myeloma. Eur J Cancer. 2006;42:1510-9.

Mateo G, Castellanos M, Rasillo A, Gutiérrez NC, Montalban MA, Martin ML, et al. Genetic abnormalities and patterns of antigenic expression in multiple myeloma. Clin Cancer Res. 2005;11:3661-7.

Rawstron AC, Orfao A, Beksac M, Bezdickova L, Brooimans RA, Bumbea H, et al. Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders. Haematologica. 2008;93:431-8.

Ocqueteau M, Orfao A, Almeida J, Blade J, González M, García-Sanz R, et al. Immunophenotypic characterization of plasma cells from monoclonal gammopathy of undetermined significance patients. Implications for the differential diagnosis between MGUS and multiple myeloma. Am J Pathol. 1998;152:1655-65.

van de Loosdrecht AA, Alhan C, Bene MC, La Porta MG, Drager AM, Feuillard J, et al. Standardization of flow cytometry in myelodysplastic syndromes: report from the first European Leukemia Net working conference on flow cytometry in myelodysplastic syndromes. Haematologica. 2009;94:1124-34.

Matarraz S, López A, Barrena S, Fernández C, Jensen E, Flores J, et al. The immunophenotype of different immature, myeloid and B-cell lineage-committed CD34+ hematopoietic cells allows discrimination between normal/reactive and myelodysplastic syndrome precursors. Leukemia. 2008;22:1175-83.

Malcovati L, Nimer SD. Myelodysplastic syndromes: diagnosis and staging. Cancer Control. 2008;15(Suppl):4-13.

Hernández-Campo PM, Almeida J, Acevedo MJ, Sánchez ML, Alberca I, Vidriales B, et al. Detailed immunophenotypic characterization of different major and minor subsets of peripheral blood cells in patients with paroxysmal nocturnal hemoglobinuria. Transfusion. 2008;48:1403-14.

Hernández-Campo PM, Almeida J, Orfao A. Paroxysmal nocturnal hemoglobinuria. Med Clin (Barc). 2008;131:617-30.

Cervantes F, Villamor N, Esteve J, Montoto S, Rives S, Rozman C, et al. ‘Lymphoid’ blast crisis of chronic myeloid leukaemia is associated with distinct clinicohaematological features. Br J Haematol. 1998;100:123-8.

Escribano L, Díaz-Agustin B, López A, Nuñez LR, García-Montero A, Almeida J, et al. Immunophenotypic analysis of mast cells in mastocytosis: When and how to do it. Proposals of the Spanish Network on Mastocytosis (REMA). Cytometry B Clin Cytom. 2004;58:1-8.

Escribano L, García AC, Nuñez R, Orfao A. Flow cytometric analysis of normal and neoplastic mast cells: role in diagnosis and follow-up of mast cell disease. Immunol Allergy Clin North Am. 2006;26:535-47.

Perfetto SP, Chattopadhyay PK, Roederer M. Seventeen-colour flow cytometry: unraveling the immune system. Nat Rev Immunol. 2004;4:648-55.

Kraan J, Gratama JW, Haioun C, Orfao A, Plonquet A, Porwit A, et al. Flow cytometric immunophenotyping of cerebrospinal fluid. Curr Protoc Cytom. 2008; Chapter 6: Unit 6.25.

How to Cite
1.
Saavedra C, Quijano SM, Romero M, Jaramillo R, Orduz R, Echeverri C, et al. Report on the First Colombian Consensus Conference for Flow Cytometric Immunophenotyping of hematological disorders. biomedica [Internet]. 2010 May 28 [cited 2024 May 17];30(Sup1):11-2. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/818
Published
2010-05-28

Altmetric

Article metrics
Abstract views
Galley vies
PDF Views
HTML views
Other views
Crossref Cited-by logo
QR Code