Evaluación de tres técnicas citogenéticas diferentes en los estudios morfométricos del carioti po de Aedes taeniorhynchus (Di ptera: culicidae

Abstract

Morphometric studies of the Aedes taeniorhynchus mosquito karyotype are of veterinary medical interest because this mosquito is a vector of epidemo-epizootic Venezuelan equine encephalitis. Three different cytogenetic techniques were used for chromosome preparation: squash, air-drying and cell culture. These three techniques were compared to each other to evaluate the obtained metaphases' chromosome length and morphology. The diploid number of the species was 6 in al1 the cariological preparations when any one of the three procedures was used. However, with the squash technique, brain-cell mytotic chromosomes were shorter, discontinuous and had less apparent grades of chromatin compaction. When air-drying was used, better cytological extensions were obtained, the chromosomes being more separated and of a slightly greater length. The best resolution was obtained using ceil culture because of the structure and separation of chromosome homology and length.