Características de una línea celular de crecimiento continuo de Aedes taeniorhynchus (Diptera: Culicidae)

Abstract

A new cell line was established, LSB-AT793, from the embryonic tissues of the mosquito Aedes taeniorhynchus. The first subculture was iniciated in July 1993. Up to the present, October 1995, 360 passages have been performed. The culture media used for cell growth and maintenance was MMNP12, with a pH of 6.8 and a temperature of 28°C. The majority of cells in the confluent monolayers were epithelial-like. The characteristics of the karyotype species were recognized and the G and C-banding patterns were established. Also, the isoenzyme profiles of the cell line, using four differents systems, PGI, PGM, MPI and 6-PGDH, were determined which coincide with the isoenzyme patterns derived from pupae extracts. The isoenzyme profiles of this cell line was compared with the isoenzyme phenotype of Anopheles albimanus and with the clone C6136. It was shown that cells are free of bacteria and fungi. The frozen technique in liquid nitrogen was standarized. This technique has allowed the storage of a variety of subcultures in different passages.