Exactitud del ELlSA para el diagnóstico de fascioliasis bovina: análisis del área bajo la curva del receptor operador (ROC)

Abstract

The enzyme-linked immuno-sorbent assay (ELISA) test for the serodiagnosis of bovine fascioliasis was standardized and evaluated. Three kinds of antigen were employed: crude somatic (SAg), total excretorykecretory (T-EISAg) and partially purified excretoty/secretory (PP-EISAg). Thirty positive samples were obtained from naturally infected canle, parasitological confirmation being positive due to the finding of adult flukes in bile ducts during post-mottem examination. The infected cattle came from regions lying between 1,800 and 2,400 meters above sea level, which are considered to be endemic for this disease. Forty-five negative samples were obtained from cattle living in non-endemic areas, whose age, breeding and growth conditions had been recorded since bitth to the actual moment at which the blood samples were taken. The receiver operator curve (ROC) was used for the evaluation of the ELlSA test, following trapezoidal integration methodology, in order to identify parasitized bovines from healthy ones. The three antigens employed -somatic (SAg), total excretory/secretoy (T-E/SAg) and partially purified excretory/secretory (PP-E/SAg)- can be used for performing fascioliasis cerodiagnosis by ELlSA test because al1 of them differentiate positive from negative samples.