Inmunodetección por peroxidasa de células de ganglio sensorial infectadas por virus de rabia
Abstract
An immunoperoxidase ABC technique was developed to detect rabies virus in dorsal root ganglion cells. This paper presents the standarization of the immunoperoxidase ABC technique which uses the antirabies conjugate produced by the Colombian lnstituto Nacional de Salud. Antibody dilution normally used in the fluorescence routine technique shows an excesive background in the ABC assay. We discuss the possible reasons and presenta protocol that minimizes the high background in the ABC immunoassay.Downloads
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How to Cite
1.
Castellanos JE, Castañeda DR, Hurtado H, Velandia AE. Inmunodetección por peroxidasa de células de ganglio sensorial infectadas por virus de rabia. biomedica [Internet]. 1996 Sep. 1 [cited 2024 May 18];16(3):207-11. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/908
Published
1996-09-01
Issue
Section
Original articles
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