Obtención y caracterización de un anticuerpo específico contra la hormona luteinizante humana

Abstract

The present work describes the production of a spectfic polyclonal human luteinising hormone (LH) antibody. The hormone was purified from a glycoprotein-rich section obtained from human hypophyses, using a previously standarised laboratory method based on differences in solubility and dielectric constant among the different proteins. The specific antiserum was generated in New Zealand rabbits and the titre was assessed by RIA. Partial characterisation of antisera was performed by determining optimum titre, affinity, specificity and its quantitative capability, as assessed in comparison with an international reference antiserum. Results showed that the antiserum obtained is suitable for the quantitation of LH by RIA.