Determinación de la actividad enzimática de la hexosaminidasa en fluidos biológicos

Abstract

This study describes a method, that uses electrophoresis for the enzyme hexosaminidase (HEX), the substrate 4-metil-umbelliferyl 2-acetamide 2-deoxi-beta Dglucopiranoside. We used samples from serum, urine and amniotic fluid, frorn normal people. We propose this method could be used as diagnostic for gangliosidoses GM2, because of the sensibility for the detection of isoenzymes HEX A and HEX B, and the capability to detect differences in fluorescence.