Extracción de ADN de Trypanosoma cruzi mediante tratamiento con bromuro de hexadecil-trimetil-amonio
Abstract
The present work describes a fast, simple and efficient method of isolating pure and easily handling of genomic DNAfrom Trypanosoma cruzi This protocol is based on parasite lysis with SDS and the removal of proteins by digestion with proteinase K, followed by polysaccharides and remaining proteins' selective precipitation with CTAB. Finally, the DNA is extracted with chloroform-isoamyl alcohol and is recovered from the aqueous supernatant by isopropanol precipitation.Downloads
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How to Cite
1.
Escalante M, Montilla M, Nicholls RS, Del Portillo P, Puerta CJ. Extracción de ADN de Trypanosoma cruzi mediante tratamiento con bromuro de hexadecil-trimetil-amonio. biomedica [Internet]. 1997 Jun. 1 [cited 2024 May 12];17(2):120-5. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/943
Published
1997-06-01
Issue
Section
Original articles
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