Preparación de columnas de afinidad con actina -F y actina-G para la obtención de proteínas de unión a actina en Plasmodium falciparum

Abstract

Affinity columns were prepared with F-actin (filaments), G-actin (monomers) and bovine serum albumin, BSA, (control) to obtain actin binding proteins from radioactively labelled Plasmodium falciparum extracts. Polymerisation was confirmed and the binding of ligands to the resin was quantified. The results show high affinity chromatography specificity as described here. Four proteins were obtained in the G-actin columns' eluted fractions and 16 in the corresponding F-actin columns' fractions.