Preliminary evaluation of maggot (Diptera: Calliphoridae) therapy as a potential treatment for leishmaniasis ulcers
Keywords:
leishmaniasis, cutaneous/therapy, therapies, investigational, debridement, larva, Ulcer
Abstract
Introduction. Maggot debridement therapy has been widely used for treating a variety of scarred-over soft-tissue wounds. Published accounts record several illnesses in which treatment with larval therapy has promoted injury healing in conjunction with infection by bacterial pathogens resistant to conventional antibiotics.Objective. An initial test of the maggot therapy was developed for cutaneous injuries produced by Leishmania amazonensis.
Materials and methods. An experimental design based on an animal model with three replicates in Mesocricetus aureatus (Rodentia: Muridae) was used to evaluate size variation lesion before and after after larval therapy with Lucilia sericata maggots. The criteria used for therapy evaluation were lesion size, maggot application time, and presence or absence of edema and secretions.
Results. Effective scarring and wound healing was observed after therapy with L. sericata larvae, i.e. 80% to 100% lesion area reduction after 12 hours.
Conclusion. The preliminary results suggest that fly maggots of L. sericata have a potential use as natural medical and veterinary alternative therapy for the cutaneous leishmaniasis.
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References
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14. Juárez E. Evaluación en sueros de la quimioterapia específica, por las técnicas de ELISA, PCR e hibridación de pacientes con leishmaniasis cutánea del Estado Trujillo (Tesis). Trujillo: Núcleo Universitario "Rafael Rangel"-Universidad de Los Andes; 2004. p. 72.
15. Ministerio de Ciencia y Tecnología. Código de Bioética y Bioseguridad. Caracas: Editorial MCT; 2002. p. 35.
16. Thomas S, Jones M. Maggots and the battle against MRSA. Bridgend: SMTL.; 2000. p. 123-40.
17. Mumcuoglou KY, Miller J, Mumcuoglu M, Friger M, Tarshis M. Destruction of bacteria in the digestive tract of the maggot of Lucilia sericata (Diptera: Calliphoridae). J Med Entomol. 2001;38:161-6.
18. Erdmann GR, Bromel M, Gassner G, Freeman TP, Fischer A. Antibacterial activity demonstrated by culture filtrates of Proteus mirabilis isolated from screwwork (Cochliomyia homivorax) (Diptera: Calliphoridae). J Med Entomol. 1984;21:159-64.
19. Erdmann GR, Khalil SK. Isolation and identification of two antibacterial agents produced by a strain of Proteus mirabilis isolated from larvae of the screwworm (Cochliomyia hominovorax) (Diptera: Calliphoridae). J Med Entomol. 1986;23:208-11.
20. Parnés A, Lagan KM. Larval therapy in wound management: A review. Int J Clin Pract. 2007;61:488-93.
21. Horobin AJ, Pritchad DL, Shakesheff LM. How do larvae of Lucilia sericata iniciate human wound healing. Eur Cell Mater. 2002;4(Suppl.2):70-1.
22. Daeschlein G, Mumcuoglu KY, Assadian O, Hoffmeister B, Kramer A. In vitro antibacterial activity of Lucilia sericata maggot secretions. Skin Pharmacol Physiol. 2007;20:112-5.
23. Figueroa L, Flores J, Rodríguez S. Método de cultivo de larvas de moscas Lucilia sericata para terapia larval. Parasitol Latinoam. 2007;62:79-82.
24. Anderson GS. Minimum and maximum development rates of some forensically important Calliphoridae (Diptera). J Forensic Sci. 2000;45:824-32.
25. Talari S, Sadr F, Doroodgar A, Talari M, Gharabagh A. Wound myasis caused by Lucilia sericata. Arch Iranian Med. 2004;2:128-9.
2. Herwald BT, Berman JD. Recommendations for treating leishmaniasis with sodium stibogluconate (pentostam) and review of pertinent clinical studies. Am J Trop Med Hyg. 1992;46:296-306.
3. Scorza JV, Morales C, Petit Y, Vásquez L, Rojas E, Scorza JV. Síntesis de un complejo antimonial pentavalente (Ulamina) y su aplicación experimental para el tratamiento de leishmaniasis cutánea localizada en Venezuela. Bol Malariol San Amb. 2006;1:59-65.
4. Bofante R, Barroeta S. Leishmanias y leishmaniasis en América con especial referencia en Venezuela. Barquisimeto: Editorial Lara; 2002. p. 270
5. Brenzan MA, Nakamura CV, Pardo Dias Filho B, Ueda-Nakamura T, Young MC, Aparacio Garcia Cortz D. Antileishmanial activity of crude extract and courin from Calophyllum brasiliense leaves against Leishmania amazonensis. Parasitol Res. 2007;101:715-22.
6. Fitzpatrick M. Tiny "surgeons" prove surprisingly effective. JAMA. 2000;284:2306-7.
7. Sherman RA, Sherman J, Gilead L, Lipo M, Mumcuoglu KY. Maggot debridement therapy in out-patients. Arch Phys Med Rehabil. 2001;82:1226-9.
8. Sherman RA, Wyle F, Vulpe M. Maggot therapy for treating pressure ulcers in spinal cord injury patients. J Spinal Cord Med. 1995;18:71-4.
9. Sherman RA. A new dressing design for use with maggot therapy. Plast Reconstr Surg. 1997;100:451-6.
10. Sherman RA. Maggot debridement in modern medicine. Infect Med. 1998;15:651-6.
11. Sherman RA, Wyle FA. Low-cost, low-maintenance rearing of maggots in hospitals, clinics and schools. Am J Trop Med Hyg. 1996;54:38-41.
12. Thomas S, Andrews AM, Hay NP, Bourgoise S. The anti-microbial activity of maggot secretions: results of a preliminary study. J Tissue Viability. 1999;9:127-32.
13. Guevara P, Rojas E, González N, Scorza JV, Añez N, Valera M, et al. Presence of Leishmania braziliensis in blood samples from cured patients to different stages of immunotherapy. Clin Diagn Lab Immunol. 1994;1: 385-9.
14. Juárez E. Evaluación en sueros de la quimioterapia específica, por las técnicas de ELISA, PCR e hibridación de pacientes con leishmaniasis cutánea del Estado Trujillo (Tesis). Trujillo: Núcleo Universitario "Rafael Rangel"-Universidad de Los Andes; 2004. p. 72.
15. Ministerio de Ciencia y Tecnología. Código de Bioética y Bioseguridad. Caracas: Editorial MCT; 2002. p. 35.
16. Thomas S, Jones M. Maggots and the battle against MRSA. Bridgend: SMTL.; 2000. p. 123-40.
17. Mumcuoglou KY, Miller J, Mumcuoglu M, Friger M, Tarshis M. Destruction of bacteria in the digestive tract of the maggot of Lucilia sericata (Diptera: Calliphoridae). J Med Entomol. 2001;38:161-6.
18. Erdmann GR, Bromel M, Gassner G, Freeman TP, Fischer A. Antibacterial activity demonstrated by culture filtrates of Proteus mirabilis isolated from screwwork (Cochliomyia homivorax) (Diptera: Calliphoridae). J Med Entomol. 1984;21:159-64.
19. Erdmann GR, Khalil SK. Isolation and identification of two antibacterial agents produced by a strain of Proteus mirabilis isolated from larvae of the screwworm (Cochliomyia hominovorax) (Diptera: Calliphoridae). J Med Entomol. 1986;23:208-11.
20. Parnés A, Lagan KM. Larval therapy in wound management: A review. Int J Clin Pract. 2007;61:488-93.
21. Horobin AJ, Pritchad DL, Shakesheff LM. How do larvae of Lucilia sericata iniciate human wound healing. Eur Cell Mater. 2002;4(Suppl.2):70-1.
22. Daeschlein G, Mumcuoglu KY, Assadian O, Hoffmeister B, Kramer A. In vitro antibacterial activity of Lucilia sericata maggot secretions. Skin Pharmacol Physiol. 2007;20:112-5.
23. Figueroa L, Flores J, Rodríguez S. Método de cultivo de larvas de moscas Lucilia sericata para terapia larval. Parasitol Latinoam. 2007;62:79-82.
24. Anderson GS. Minimum and maximum development rates of some forensically important Calliphoridae (Diptera). J Forensic Sci. 2000;45:824-32.
25. Talari S, Sadr F, Doroodgar A, Talari M, Gharabagh A. Wound myasis caused by Lucilia sericata. Arch Iranian Med. 2004;2:128-9.
How to Cite
1.
Arrivillaga J, Rodríguez J, Oviedo M. Preliminary evaluation of maggot (Diptera: Calliphoridae) therapy as a potential treatment for leishmaniasis ulcers. biomedica [Internet]. 2008 Jun. 1 [cited 2024 May 16];28(2):305-10. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/102
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