Detection and expression of a calmodulin binding protein from Plasmodium falciparum
Keywords:
plasmodium falciparum, calmodulin binding proteins
Abstract
Monoclonal antibodies were produced against affinity chromatography purified calmodulin binding proteins (CaMBP) of Plasmodium falciparum. Using one of them (1G3) in a Western blot assay, a 30 kDa calmodulin binding protein was detected in extracts of erythrocytes infected with Plasmodium falciparum. This protein was not detected in extracts of non infected erythrocytes. Additionally, the antibody presented a cross reaction with two bands 260 and 240 kDa, that reacted also with anti-spectrin policlonal antibody, suggesting that a similar epitope exists in the 30 kDa CaMBP and in spectrin. A study of expression of the 30 kDa CaMBP during the erythrocytic cell cycle showed a stage-specific expression pattern, detecting it only in the late stages of the parasite and not in the younger ones.Downloads
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How to Cite
1.
Guerra AP, Vera VJ, Rojas MO, Wasserman M. Detection and expression of a calmodulin binding protein from Plasmodium falciparum. biomedica [Internet]. 2001 Mar. 1 [cited 2024 May 17];21(1):41-52. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/1086
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Published
2001-03-01
Issue
Section
Original articles
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