Genotyping and evaluation of infection dynamics in a Colombian isolate of Leptospira santarosai in hamster as an experimental model
Abstract
Introduction: Is necessary to develop models for the study of leptospirosis.
Objective: To genotype a Colombian strain of Leptospira isolated from a human with Weil’s syndrome and to evaluate its infection dynamics in the hamster experimental model.
Materials and methods: Genotyping was performed by amplification and sequence analysis of the rrs 16S and lipL32 genes. The median lethal dose was determined in intraperitoneally inoculated hamsters. The patterns of clinical chemistry, the duration of leptospiremia, leptospiruria and pathological findings were studied and compared in the same animal model infected with L. interrogans (Fiocruz L1-130).
Results: Molecular typing revealed that the isolate corresponded to the pathogenic species L. santarosai, which was recovered from hamsters´ kidneys and lungs and detected by lipL32 PCR from day 3 post-infection in these organs. There was a marked increase of C-reactive protein in animals at day 5 post-infection (3.25 mg/dl; normal value: 0.3 mg/dl) with decreases by day 18 (2.60 mg/dl: normal value: 0.8 mg/dl). Biomarkers of urea showed changes consistent with possible renal acute failure (day 5 post-infection: 49.01 mg/dl and day 18 post-infection: 53.71 mg/dl). Histopathological changes included interstitial pneumonia with varying degrees of hemorrhage and interstitial nephritis.
Conclusion: The pathogenic species L. santarosai was identified in Colombia. Its pathogenicity as determined by tropism to lung and kidney was comparable to that of L. interrogans Fiocruz L1-130, well known for its virulence and pulmonar tropism. The biological aspects studied here had never before been evaluated in an autochthonous isolate.
Downloads
Some similar items:
- Piedad Agudelo Flórez, Marcos Restrepo, María Amparo Lotero, Evaluation of indirect immunofluorescence assay for diagnosis of human leptospirosis. , Biomedica: Vol. 26 No. 2 (2006)
- Lina María Echeverri-Toro, Sara Penagos, Laura Castañeda, Pablo Villa, Santiago Atehortúa, Faiver Ramírez, Carlos Restrepo, Sigifredo Ospina, Yuli Agudelo, Alicia Hidrón, Paola Agudelo, Eliana Valderrama, Carlos Andrés Agudelo, Sociodemographic and clinical characteristics of patients infected with Leptospira spp. treated at four hospitals in Medellín, Colombia, 2008-2013 , Biomedica: Vol. 37 No. 1 (2017)
- Piedad Agudelo-Flórez, Marcos Restrepo, Natalí Moreno, Diagnosis of leptospirosis by dark-field microscopy of blood samples and culture , Biomedica: Vol. 28 No. 1 (2008)
- María Teresa Arango, Carlos Jaramillo, María Camila Montealegre, Mabel Helena Bohórquez, María del Pilar Delgado, Genetic characterization of the interleukin 1 β polymorphisms -511, -31 y +3954 in a Colombian population with dyspepsia , Biomedica: Vol. 30 No. 2 (2010)
- Diana Marín, Eduardo Romero, Virtual microscopy systems: Analysis and perspectives , Biomedica: Vol. 31 No. 1 (2011)
- Daniel Echeverri, María de los Ángeles Vargas, Lorena Matta, Fernando Rosso, Janier Daniel Segura, Invasive Streptococcus pneumoniae infection (Austrian syndrome): a case report , Biomedica: Vol. 35 No. 1 (2015)
- Solmara Bello, Milena Rodríguez, Andrea Paredes, Fredy Mendivelso, Diana Walteros, Flor Rodríguez, María Elena Realpe, Epidemiological surveillance of human leptospirosis in Colombia, 2007-2011 , Biomedica: Vol. 33 (2013): Suplemento 1, Fiebres hemorrágicas
- Claudia M. Romero-Vivas, Dorothy Thiry, Virginia Rodríguez, Alfonso Calderón, Germán Arrieta, Salim Mattar, Margarett Cuello, Paul N. Levett, Andrew K. Falconar, Molecular serovar characterization of Leptospira isolates from animals and water in Colombia , Biomedica: Vol. 33 (2013): Suplemento 1, Fiebres hemorrágicas
- Ana Lucía Rodríguez, Beatriz Eugenia Ferro, María Ximena Varona, Mauricio Santafé, Exposure to Leptospira in stray dogs in the city of Cali. , Biomedica: Vol. 24 No. 3 (2004)
- Beatriz Eugenia Ferro, Ana Lucía Rodríguez, Mauricio Pérez, Bruno Luis Travi, Seroprevalence of Leptospira infection in habitants of peripheral neighborhoods in Cali, Colombia. , Biomedica: Vol. 26 No. 2 (2006)
Article metrics | |
---|---|
Abstract views | |
Galley vies | |
PDF Views | |
HTML views | |
Other views |