Genetic differentiation of three Colombian populations of Triatoma dimidiata (Heteroptera: Reduviidae) by ND4 mitochondrial gene molecular analysis
Keywords:
Chagas disease, Triatoma, Triatominae, genetics, population, polymorphism, NADH dehydrogenase
Abstract
Introduction. Triatoma dimidiata is the second most important vector of Chagas disease in Colombia after Rhodnius prolixus. Population genetic studies are essential for the adequate design and implementation of vector control and surveillance strategies.Objective. The level of genetic variability and population differentiation was surveyed among three Colombian populations of T. dimidiata from different geographic locations and ecotopes, using ND4 mitochondrial gene.
Materials and methods. Genetic comparison was made between two wild populations from La Guajira (n=10) and Santander (n=10) provinces, and one intra (n=15) and one peridomiciliary (n=5) population from the Cesar province. The polymorphism frequencies of the ND4 mitochondrial gene sequence were analyzed to deduce population structure based on the 40 samples.
Results. Colombian T. dimidiata showed a high nucleotide (π: 0.034) and haplotype diversity (Hd: 0.863), as well as significant population subdivision (fST: 0.761) and a low migration rate (Nm: 0.157).
Genetic distances and variability differences among populations indicate distinct population subdivision amongst the three provinces.
Conclusion. ND4 proved useful in elucidating the significant genetic differentiation that has occurred among T. dimidiata populations from La Guajira, Cesar and Santander. The analysis suggested a relationship between population subdivision and some eco-epidemiological attributes of this vector from the central eastern and northwestern regions of Colombia.
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References
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2. Dorn P, Monroy C, Curtis A. Triatoma dimidiata (Latreille, 1811): A review of its diversity across its geographic range and the relationship among populations. Inf Gen Evol. 2007;7:343-52.
3. Bargues MD, Guhl F, Mas-Coma S. Genetic characterization of domestic, peridomestic and sylvatic Triatoma dimidiata populations from Colombia by ribosomal DNA ITS-2 sequence analyses. Acta Trop. 2002;83:149.
4. Ramírez C, Jaramillo C, Delgado P, Pinto N, Aguilera G, Guhl F. Genetic structure of sylvatic, peridomestic and domestic populations of Triatoma dimidiata (Hemiptera: Reduviidae) from an endemic zone of Boyacá, Colombia. Acta Trop. 2005;93:23-9.
5. Guhl F, Aguilera G, Pinto N, Vergara D. Actualización de la distribución geográfica y ecoepidemiología de la fauna de triatominos (Reduviidae: Triatominae) en Colombia. Biomédica. 2007;27(Suppl.1):143-62.
6. Angulo VM. Ensayo de estrategias de control y vigilancia de Triatoma dimidiata en Colombia. En: Guhl F, editor. Memorias del Primer Taller Internacional sobre Control de la Enfermedad de Chagas. Bogotá, D.C; Universidad de los Andes: 2005. p. 91-102.
7. Parra-Henao G, Angulo V, Jaramillo N, Restrepo M. Triatominos (Hemiptera: Reduviidae) de La Sierra Nevada de Santa Marta, Colombia. Aspectos epidemiológicos, entomológicos y de distribución. CES Med. 2009;23:17-26.
8. Bargues MD, Klisiowicz D, González-Candelas F, Ramsey JM, Monroy C, et al. Phylogeography and genetic variation of Triatoma dimidiata, the main Chagas disease vector in Central America, and its position within the genus Triatoma. PLoS Negl Trop Dis. 2008;2:e233. doi:10.1371/journal.pntd.0000233.
9. Dorn PL, Calderón C, Melgar S, Moguel B, Solorzano E. Two distinct Triatoma dimidiata (Latreille, 1811) taxa are found in sympatry in Guatemala and Mexico. PLoS Negl Trop Dis. 2009;3:e393. doi:10.1371/journal.pntd.0000393.
10. Gorrochotegui-Escalante N, De Lourdes M, Fernández-Salas I, Beaty B, Black W. Genetic isolation by distance among Aedes aegypti populations along the northeastern coast of Mexico. Am J Trop Med Hyg. 2000;62:200-9.
11. Uribe S, Lehmann T, Rowton E, Vélez I, Porter C. Speciation and population structure in the morphospecies Lutzomyia longipalpis (Lutz & Neiva) as derived from the mitochondrial ND4 gene. Mol Phylogen Evol. 2001;18:84-93.
12. Depaquit J, Lienard E, Verzeaux-Griffon A, Ferté H, Bounamous A, Gantier JC, et al. Molecular homogeneity in diverse geographical populations of Phlebotomus papatasi (Diptera: Psychodidae) inferred from ND4 mtDNA and ITS2 rDNA epidemiological consequences. Inf Gen Evol. 2008;8:159-70.
13. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae) and their significance as vectors of Chagas disease. Bull Am Mus Nat His. 1979;163:123-520.
14. Collins FH, Méndez MA, Rasmussen MO, Mehaffey PC, Besansky NJ, Finnerty V. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Am J Trop Med Hyg. 1987;37:37-41.
15. Lyman D, Monteiro F, Escalante A, Cordon C, Wesson D, Dujardin J, Beard C. Mitochondrial DNA sequence variation among Triatominae vectors of Chagas disease. Am J Trop Med Hyg. 1999;60:377-86.
16. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;25:4876-82.
17. Hall T. BioEdit: a user-mendly biological sequences alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-8.
18. Dotson E, Beard C. Sequence and organization of the mitochondrial genome of the Chagas disease vector, Triatoma dimidiata. Insect Mol Biol. 2001;10:205-15.
19. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596-9.
20. Rozas J, Sánchez-DelBarrio J, Messeguer X, Rozas R. DnaSP v 4.5, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 2003;19:2496-7.
21. Nei M. Molecular evolutionary genetics. New York: Columbia University Press; 1987.
22. Xia X, Xie Z. DAMBE: Data analysis in molecular biology and evolution. J Hered. 2001;92:371-3.
23. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10:512-26.
24. Excoffier L, Smouse PE, Quattro JM. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics. 1992;131:479-91.
25. Peakall R, Smouse P. GENALEX 6: genetic analysis in Excel, population genetic software for teaching and research. Mol Ecol Notes. 2006;6:288-95.
26. Wright S. Evolution and the Genetics of Populations, vol. 4, Variability within and among natural populations. Chicago, IL: University of Chicago Press. 1978.
27. Frankham R, Briscoe D, Ballou J. Introduction to conservation genetics. New York: Cambridge University Press; 2002.
28. Pfeiler E, Bitler B, Ramsey J, Palacios-Cardiel C, Markow T. Genetic variation, population structure, and phylogenetic relationships of Triatoma rubida and T. recurva (Hemiptera: Reduviidae: Triatominae) from the Sonoran desert, insects vectors of the Chagas' Disease parasite Trypanosoma cruzi. Mol Phyl Evol. 2006;41:209-21.
How to Cite
1.
Grisales N, Triana O, Angulo V, Jaramillo N, Parra-Henao G, Panzera F, et al. Genetic differentiation of three Colombian populations of Triatoma dimidiata (Heteroptera: Reduviidae) by ND4 mitochondrial gene molecular analysis. biomedica [Internet]. 2010 Aug. 4 [cited 2024 May 18];30(2):207-14. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/184
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