Comparison of 16S and COX1 genes mitochondrial regions and their usefulness for genetic analysis of ticks (Acari: Ixodidae)
Abstract
Introduction: In recent decades the analysis of mitochondrial genes has been used for population and phylogenetic studies of ticks allowing many advances in their systematics. Mitochondrial ribosomal 16S (16S) subunit is one of the most frequently used among those genes available for tick analysis, whereas cytochrome oxidase gene 1 (COX1) has recently been used and proposed as an alternative to the traditional 16S gene marker. Objective: To evaluate the usefulness of 16S and COX1 in genetic studies of ticks by analyzing sequences of three species commonly found in the Caribbean region of Colombia. Results: The analysis of both genes sequences allowed us to identify the three species with high levels of confidence and interspecific genetic divergence (19-22%), although only COX1 allowed us to detect intraspecific genetic variability (up to ~0.8%). A substitution saturation analysis indicated that the 16S gene was not saturated with transitions while the COX1 gene showed saturation distances starting at ~17%. Conclusion: Our results indicated that the 16S gene seems to have better features for interspecific phylogenetic analyses because of its high level of genetic divergence and low saturation pattern, while the COX1 gene appears to be more useful for intraspecific genetic variability studies. However, as our study was conducted at a local scale, future studies at different biogeographical scales would help to establish its usefulness in wider and more complex scenarios.
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Andrade L, Caraveo-Anduaga JJ, Berglund P, Bijl RV, De Graaf R, Vollebergh W, et al . The epidemiology of major depressive episodes: Results from the International Consortium of Psychiatric Epidemiology (ICPE) Surveys. Int J Methods Psychiatr Res. 2003;12:3-21. http://dx.doi.org/10.1002/mpr.138
Ministerio de la Protección Social de Colombia. Estudio Nacional de Salud Mental, Colombia, 2003. Accessed: May 15, 2011. Available from: http://onsm.ces.edu.co/uploads/files/1243030_EstudioNacionalSM2003.pdf.
Mathers CD, Loncar D. Updated projections of global mortality and burden of disease, 2002-2030: Data sources, methods and results, 2005. Accessed: September 24, 2012. Available from: http://www.who.int/healthinfo/statistics/ bodprojectionspaper.pdf.
World Health Organization WHO. The Global Burden of Disease: 2004 update. Accessed: June 10, 2012. Available from: http://www.who.int/healthinfo/global_burden_disease/GBD_report_2004update_full.pdf.
Kessler RC, Greenberg PE, Mickelson KD, Meneades LM, Wang PS. The effects of chronic medical conditions on work loss and work cutback. J Occup Environ Med. 2001;43:218-25.
Punakallio A, Lusa S, Luukkonen R, Airila A, Leino-Arjas P. Musculoskeletal pain and depressive symptoms as predictors of trajectories in work ability among finnish firefighters at 13-year follow-up. J Occup Environ Med. 2014;56:367-75. http://dx.doi.org/10.1097/JOM.0000000000000139
Acosta N. Carga de enfermedad, Colombia, 2005: resultados alcanzados. Documento técnico ASS/1502-08. 2008. Accessed: February 10, 2011. Available from: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/INEC/UNIV/PresentacionCarga_Informe2005.pdf.
Ebmeier KP, Donaghey C, Steele JD. Recent developments and current controversies in depression. Lancet. 2006;367:153-67. http://dx.doi.org/10.1016/S0140-6736(06)67964-6
Sullivan PF, Neale MC, Kendler KS. Genetic epidemiology of major depression: Review and meta-analysis. Am J Psychiatry. 2000;157:1552-62. http://dx.doi.org/10.1176/appi.ajp.157.10.1552
McGrath LM, Cornelis MC, Lee PH, Robinson EB, Duncan LE, Barnett JH, et al. Genetic predictors of risk and resilience in psychiatric disorders: A cross-disorder genome-wide association study of functional impairment in major depressive disorder, bipolar disorder, and schizophrenia. Am J Med Genet B Neuropsychiatr Genet. 2013;162B:779-88. http://dx.doi.org/10.1002/ajmg.b.32190
Levinson DF. The genetics of depression: A review. Biol Psychiatry. 2006;60:84-92. http://dx.doi.org/10.1016/j.biopsych.2005.08.024
Kendler KS, Gardner CO, Neale MC, Prescott CA. Genetic risk factors for major depression in men and women: Similar or different heritabilities and same or partly distinct genes? Psychol Med. 2001;31:605-16.
Hariri AR, Holmes A. Genetics of emotional regulation: The role of the serotonin transporter in neural function. Trends Cogn Sci. 2006;10:182-91. http://dx.doi.org/10.1016/j.tics.2006.02.011
Lesch KP, Bengel D, Heils A, Sabol SZ, Greenberg BD, Petri S, et al. Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region. Science. 1996;274:1527-31. http://dx.doi.org/10.1126/science.274.5292.1527
Neumeister A, Young T, Stastny J. Implications of genetic research on the role of the serotonin in depression: Emphasis on the serotonin type 1A receptor and the serotonin transporter. Psychopharmacology (Berl). 2004;174:512-24. http://dx.doi.org/10.1007/s00213-004-1950-3
Stockmeier CA. Involvement of serotonin in depression: Evidence from postmortem and imaging studies of serotonin receptors and the serotonin transporter. J Psychiatr Res. 2003;37:357-73. http://dx.doi.org/10.1016/S0022-3956(03)00050-5
Serretti A, Calati R, Mandelli L, De Ronchi D. Serotonin transporter gene variants and behavior: A comprehensive review. Curr Drug Targets. 2006;7:1659-69. http://dx.doi.org/10.2174/138945006779025419
Furlong RA, Ho L, Walsh C, Rubinsztein JS, Jain S, Paykel ES, et al . Analysis and meta-analysis of two serotonin transporter gene polymorphisms in bipolar and unipolar affective disorders. Am J Med Genet. 1998;81:58-63. http://dx.doi.org/10.1002/(SICI)1096-8628(19980207)81:1<58::AID-AJMG11>3.0.CO;2-V
Haenisch B, Herms S, Mattheisen M, Steffens M, Breuer R, Strohmaier J, et al . Genome-wide association data provide further support for an association between 5-HTTLPR and major depressive disorder. J Affect Disord. 2013;146:438-40. http://dx.doi.org/10.1016/j.jad.2012.08.001.
Anguelova M, Benkelfat C, Turecki G. A systematic review of association studies investigating genes coding for serotonin receptors and the serotonin transporter: I. Affective disorders. Mol Psychiatry. 2003;8:574-91. http://dx.doi.org/10.1038/sj.mp.4001328
López-León S, Janssens AC, González-Zuloeta Ladd AM, Del-Favero J, Claes SJ, Oostra BA, et al. Meta-analyses of genetic studies on major depressive disorder. Mol Psychiatry. 2008;13:772-85. http://dx.doi.org/10.1038/sj.mp.4002088
Anguelova M, Benkelfat C, Turecki G. A systematic review of association studies investigating genes coding for serotonin receptors and the serotonin transporter: II. Suicidal behavior. Mol Psychiatry. 2003;8:646-53. http://dx.doi.org/10.1038/sj.mp.4001336
Lasky-Su JA, Faraone SV, Glatt SJ, Tsuang MT. Meta-analysis of the association between two polymorphisms in the serotonin transporter gene and affective disorders. Am J Med Genet B Neuropsychiatr Genet. 2005;133B:110-5. http://dx.doi.org/10.1002/ajmg.b.30104
Hoehe MR, Wendel B, Grunewald I, Chiaroni P, Levy N, Morris-Rosendahl D, et al . Serotonin transporter ( 5-HTT ) gene polymorphisms are not associated with susceptibility to mood disorders. Am J Med Genet. 1998;81:1-3. http://dx. doi.org/10.1002/(SICI)1096-8628(19980207)81:1<1::AID-AJMG1>3.0.CO;2-2
Minov C, Baghai TC, Schüle C, Zwanzger P, Schwarz MJ, Zill P, et al . Serotonin-2A-receptor and -transporter polymorphisms: Lack of association in patients with major depression. Neurosci Lett. 2001;303:119-22. http://dx.doi.org/10.1016/S0304-3940(01)01704-9
Mendlewicz J, Massat I, Souery D, Del-Favero J, Oruc L, Nöthen MM, et al . Serotonin transporter 5HTTLPR polymorphism and affective disorders: No evidence of association in a large European multicenter study. Eur J Hum Genet. 2004;12:377-82. http://dx.doi.org/10.1038/sj.ejhg.5201149
Serretti A, Lilli R, Lorenzi C, Lattuada E, Cusin C, Smeraldi E. Serotonin transporter gene (5-HTTLPR) and major psychoses. Mol Psychiatry. 2002;7:95-9. http://dx.doi.org/10.1038/sj/mp/4000936
Ospina-Duque J, Duque C, Carvajal-Carmona L, Ortíz-Barrientos D, Soto I, Pineda N, et al . An association study of bipolar mood disorder (type I) with the 5-HTTLPR serotonin transporter polymorphism in a human population isolate from Colombia. Neurosci Lett. 2000;292:199-202. http://dx.doi.org/10.1016/S0304-3940(00)01464-6
Rengifo L, Gaviria D, Salazar L, Vélez JP, Lozano S. Polimorfismos en el gen del transportador de serotonina (SLC6A4) y el trastorno afectivo bipolar en dos centros regionales de salud mental del Eje Cafetero. Revista Colombiana de Psiquiatría. 2012;41:86-100.
Escobar CG, Calderón JH, Moreno GA. Allelic polymorphism in the serotonin transporter gene in major depression patients. Colombia Médica. 2011;42:48-53.
Akhtar-Danesh N, Landeen J. Relation between depression and sociodemographic factors. Int J Ment Health Syst. 2007;1:4. http://dx.doi.org/10.1186/1752-4458-1-4
Ho LW, Furlong RA, Rubinsztein JS, Walsh C, Paykel ES, Rubinsztein DC. Genetic associations with clinical characteristics in bipolar affective disorder and recurrent unipolar depressive disorder. Am J Med Genet. 2000;96: 36-42. http://dx.doi.org/10.1002/(SICI)1096-8628(20000207)96:1<36::AID-AJMG8>3.0.CO;2-6
Pfeiffer PN, Ganoczy D, Ilgen M, Zivin K, Valenstein M. Comorbid anxiety as a suicide risk factor among depressed veterans. Depress Anxiety. 2009;26:752-7. http://dx.doi.org/10.1002/da.20583
Hirschfeld RM. The comorbidity of major depression and anxiety disorders: Recognition and management in primary care. Prim Care Companion J Clin Psychiatry. 2001;3:244-54.
Lau JY, Goldman D, Buzas B, Fromm SJ, Guyer AE, Hodgkinson C, et al . Amygdala function and 5-HTT gene variants in adolescent anxiety and major depressive disorder. Biol Psychiatry. 2009;65:349-55. http://dx.doi.org/10.1016/j.biopsych.2008.08.037
Ressler KJ, Nemeroff CB. Role of serotonergic and noradrenergic systems in the pathophysiology of depression and anxiety disorders. Depress Anxiety. 2000;12(Suppl.1):2-19. http://dx.doi.org/10.1002/1520-6394(2000)12:1+<2::AID-DA2>3.0.CO;2-4
Murphy SE, Norbury R, Godlewska BR, Cowen PJ, Mannie ZM, Harmer CJ, et al . The effect of the serotonin transporter polymorphism (5-HTTLPR) on amygdala function: A meta-analysis. Mol Psychiatry. 2013;18:512-20. http://dx.doi.org/10.1038/mp.2012.19
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