Patrón de los motivos EPIYA de cepas cubanas de Helicobacter pylori positivas para CagA

Lino E. Torres, Lidice González, Karelia Melián, Jordis Alonso, Arlenis Moreno, Mayrín Hernández, Orlando Reyes, Ludisleydis Bermúdez, Javier Campos, Guillermo Pérez-Pérez, Boris L. Rodríguez, .

Palabras clave: Helicobacter pylori, neoplasias gástricas, factores de virulencia, Cuba

Resumen

Introducción. Se sabe que el polimorfismo en la región C-terminal de la citotoxina asociada al gen A (CagA) influye en el desarrollo de la enfermedad gástrica durante la infección por Helicobacter pylori.
Objetivo. Determinar el número y el tipo de patrones de fosforilación de CagA (patrón EPIYA) en aislamientos cubanos de H. pylori, y estudiar su asociación con las enfermedades gástricas.
Materiales y métodos. Se empleó el ADN de 95 cepas de H. pylori positivas para CagA, para amplificar la región 3’ variable del gen cagA por PCR, mediante el empleo de diferentes estrategias. Además, se diseñaron nuevos cebadores para clasificar por PCR los aislamientos según el tipo de CagA, occidental o del este asiático. Los productos de PCR obtenidos de 14 aislamientos representativos se purificaron y secuenciaron para confirmar los resultados de la PCR.
Resultados. La distribución de los patrones EPIYA encontrada, fue: 2 AB (2,1 %), 1 AC (1,1 %), 1 BC (1,1 %), 70 ABC (73,6 %), 19 ABCC (20 %), y 2 ABCCC (2,1 %). El análisis de la secuenciación confirmó las clasificaciones hechas por PCR en las 14 cepas estudiadas y demostró tres cepas con secuencias únicas de nucleótídos, no reportadas anteriormente. La distribución del patrón EPIYA-ABC fue equivalente en todas las enfermedades encontradas: 78,9 % en úlcera gástrica, 72,5 % en úlcera duodenal y 72,2 % en dispepsia no ulcerada.
Conclusión. La mayoría de los aislamientos cubanos presentaron las combinaciones de motivos EPIYA menos virulentas (ABC). Los resultados del empleo de los nuevos cebadores y el análisis de la secuenciación, confirmaron que todas las cepas estudiadas portaban el gen cagA de tipo occidental.
Ninguno de los patrones específicos de EPIYA se asoció con úlcera péptica. Este es el primer reporte que muestra la distribución de los motivos EPIYA en los aislamientos de H. pylori de la región del Caribe.

 

DOI: http://dx.doi.org/10.7705/biomedica.v32i1.453

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  • Lino E. Torres Department of Research and Development, Division of Production and Development of Equipments and Diagnostics, National Centre for Scientific Research, Ciudad de La Habana, Cuba
  • Lidice González Department of Research and Development, Division of Production and Development of Equipments and Diagnostics, National Centre for Scientific Research, Ciudad de La Habana, Cuba
  • Karelia Melián Department of Gastroenterology, Medical and Surgery Research Centre (CIMEQ), Ciudad de La Habana, Cuba
  • Jordis Alonso Department of Gastroenterology, Medical and Surgery Research Centre (CIMEQ), Ciudad de La Habana, Cuba
  • Arlenis Moreno Department of Research and Development, Division of Production and Development of Equipments and Diagnostics, National Centre for Scientific Research, Ciudad de La Habana, Cuba
  • Mayrín Hernández Department of Research and Development, Division of Production and Development of Equipments and Diagnostics, National Centre for Scientific Research, Ciudad de La Habana, Cuba
  • Orlando Reyes Department of Research and Development, Division of Production and Development of Equipments and Diagnostics, National Centre for Scientific Research, Ciudad de La Habana, Cuba
  • Ludisleydis Bermúdez Department of Research and Development, Division of Production and Development of Equipments and Diagnostics, National Centre for Scientific Research, Ciudad de La Habana, Cuba
  • Javier Campos Department of Molecular Biology, Biotechnology Division, National Centre for Scientific Research, Ciudad de La Habana, Cuba
  • Guillermo Pérez-Pérez Departments of Medicine and Microbiology, New York University, School of Medicine, USA
  • Boris L. Rodríguez Department of Research and Development, Division of Production and Development of Equipments and Diagnostics, National Centre for Scientific Research, Ciudad de La Habana, Cuba

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Cómo citar
1.
Torres LE, González L, Melián K, Alonso J, Moreno A, Hernández M, et al. Patrón de los motivos EPIYA de cepas cubanas de Helicobacter pylori positivas para CagA. biomedica [Internet]. 1 de marzo de 2012 [citado 28 de marzo de 2024];32(1):23-31. Disponible en: https://revistabiomedica.org/index.php/biomedica/article/view/453

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