SARS-CoV-2 and RT-PCR in asymptomatic patients: Results of a cohort of workers at El Dorado International Airport in Bogotá, 2020
Abstract
Introduction: The 2019 coronavirus pandemic (COVID-19) has caused around 25 million cases worldwide. Asymptomatic patients have been described as potential sources of transmission. However, there are difficulties to detect them and to establish their role in the dynamics of virus transmission, which hinders the implementation of prevention strategies.
Objective: To describe the behavior of asymptomatic SARS-CoV-2 virus infection in a cohort of workers at the El Dorado “Luis Carlos Galán Sarmiento” International Airport in Bogotá, Colombia.
Materials and methods: A prospective cohort of 212 workers from the El Dorado airport was designed. The follow-up began in June, 2020. A survey was used to characterize health and work conditions. Every 21 day, a nasopharyngeal swab was taken to identify the presence of SARS-CoV-2 using RT-PCR. We analyzed the behavior of the cycle threshold (ORF1ab and N genes) according to the day of follow-up.
Results: In the first three follow-ups of the cohort, we found an incidence of SARS-CoV-2 infection of 16.51%. The proportion of positive contacts was 14.08%. The median threshold for cycle threshold was 33.53.
Conclusion: We characterized the asymptomatic SARS-CoV-2 infection in a cohort of workers. The identification of asymptomatic infected persons continues to be a challenge for epidemiological surveillance systems.
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References
World Health Organization. Coronavirus disease (COVID-19). Weekly Epidemiological Update. Geneva: World Health Organization; 2020.
Sun Y, Dong Y, Wang L, Xie H, Li B, Chang C, et al. Characteristics and prognostic factors of disease severity in patients with COVID-19: The Beijing experience. J Autoimmun. 2020;112:102473. https://doi.org/10.1016/j.jaut.2020.102473
Baloch S, Baloch M, Zheng T, Pei X. The coronavirus disease 2019 (COVID-19) pandemic. Tohoku J Exp Med. 2020;250:271-8. https://doi.org/10.1620/tjem.250.271
Borges do Nascimento I, Cacic N, Abdulazeem H, von Groote T, Jayarajah U, Weerasekara I, et al. Novel coronavirus infection (COVID-19) in humans: A scoping review and metaanalysis. J Clin Med. 2020;9:941. https://doi.org/10.3390/jcm9040941
Padhi A, Kumar S, Gupta E, Saxena SK. Laboratory diagnosis of novel coronavirus disease 2019 (COVID-19) infection. In: Saxena S, editor. Coronavirus disease 2019 (COVID-19). Medical virology: From pathogenesis to disease control. Singapore: Springer; 2020. https://doi.org/10.1007/978-981-15-4814-7_9
Arons M, Hatfield K, Reddy S, Kimball A, James A, Jacobs JR, et al. Presymptomatic SARS-CoV-2 infections and transmission in a skilled nursing facility. N Engl J Med. 2020;382:208190. https://doi.org/10.1056/NEJMoa2008457
Ki M. Epidemiologic characteristics of early cases with 2019 novel coronavirus (2019-nCoV) disease in Korea. Epidemiol Health. 2020;42:e2020007. https://doi.org/10.4178/epih.e2020007
Gao Z, Xu Y, Sun C, Wang X, Guo Y, Qiu S, et al. A systematic review of asymptomatic infections with COVID-19. J Microbiol Immunology Infect. 2020. https://doi.org/10.1016/j.jmii.2020.05.001
Zhao H, Lu X, Deng Y, Tang Y, Lu J. COVID-19: asymptomatic carrier transmission is an underestimated problem. Epidemiol Infect. 2020;148:e116. https://doi.org/10.1017/S0950268820001235
Bai Y, Yao L, Wei T, Tian F, Jin D-Y, Chen L, et al. presumed asymptomatic carrier transmission of COVID-19. JAMA. 2020;323:1406. https://doi.org/10.1001/jama.2020.2565
McKibbin W, Fernando R. The global macroeconomic impacts of COVID-19: Seven scenarios. SSRN Journal. 2020. https://doi.org/10.2139/ssrn.3547729
Nurchis M, Pascucci D, Sapienza M, Villani L, D’Ambrosio F, Castrini F, et al. Impact of the burden of COVID-19 in Italy: Results of disability-adjusted life years (DALY) and productivity loss. Int J Environ Res Public Health. 2020;17:4233. https://doi.org/10.3390/ijerph17124233
Gostic K, Gomez A, Mummah R, Kucharski A, Lloyd-Smith J. Estimated effectiveness of symptom and risk screening to prevent the spread of COVID-19. eLife 2020;9:e55570. https://doi.org/10.7554/eLife.55570
Vidal-Anzardo M, Solis G, Solari L, Minaya G, Ayala-Quintanilla B, Astete-Cornejo J, et al. Evaluación en condiciones de campo de una prueba serológica rápida para detección de anticuerpos IgM e IgG contra SARS-CoV-2. Rev Peru Med Exp Salud Pública. 2020;37:203-9. https://doi.org/10.17843/rpmesp.2020.372.5534
Fields BKK, Demirjian NL, Gholamrezanezhad A. Coronavirus disease 2019 (COVID-19) diagnostic technologies: A country-based retrospective analysis of screening and containment procedures during the first wave of the pandemic. Clin Imaging. 2020;67:219-25. https://doi.org/10.1016/j.clinimag.2020.08.014
Burdorf A, Porru F, Rugulies R. The COVID-19 (Coronavirus) pandemic: consequences for occupational health. Scand J Work Environ Health. 2020;46:229-230. https://doi.org/10.5271/sjweh.3893
Dean AG, Sullivan KM, Soe MM. OpenEPi: Open source epidemiologic statistics for public health. Fecha de consulta: 5 de septiembre de 2020. Disponible en: https://www.openepi.com/Menu/OE_Menu.htm
Fuentes-Ferrer ME, Prado-González N del. Medidas de frecuencia y de asociación en epidemiología clínica. Anales de Pediatría Continuada. 2013;11:346-9. https://doi.org/10.1016/S1696-2818(13)70157-4
DataCamp. Boxplot function | R Documentation. Boxplots. 2020. Fecha de consulta: 5 de septiembre de 2020. Disponible en: https://www.rdocumentation.org/packages/graphics/versions/3.6.2/topics/boxplot
Braithwaite I, Callender T, Bullock M, Aldridge RW. Automated and partly automated contact tracing: A systematic review to inform the control of COVID-19. Lancet Digit Health. 2020 (on line, ahead of print) https://doi.org/10.1016/S2589-7500(20)30184-9
Afzal I, Abdul Raheem R, Rafeeq N, Moosa S. Contact tracing for containment of novel coronavirus disease (COVID-19) in the early phase of the epidemic in the Maldives. Asia Pac J Public Health. 2020;100:112-6. https://doi.org/10.1177/1010539520956447
Nikolai LA, Meyer CG, Kremsner PG, Velavan TP. Asymptomatic SARS coronavirus 2 infection: Invisible yet invincible. Int J Infect Dis. 2020. https://doi.org/10.1016/j.ijid.2020.08.076
Ye F, Xu S, Rong Z, Xu R, Liu X, Deng P, et al. Delivery of infection from asymptomatic carriers of COVID-19 in a familial cluster. Int J Infect Dis. 2020;94:133-8. https://doi.org/10.1016/j.ijid.2020.03.042
Wei L, Lin J, Duan X, Huang W, Lu X, Zhou J, et al. Asymptomatic COVID-19 patients can contaminate their surroundings: An environment sampling study. mSphere. 2020;5:e00442-20. https://doi.org/10.1128/mSphere.00442-20
Opain PLM. Medidas de prevención para el COVID-19 en el Aeropuerto Internacional El Dorado | Bogotá, Colombia. Fecha de consulta: 5 de septiembre de 2020. Disponible en: https://eldorado.aero/servicios/coronavirus
Chu DK, Akl EA, Duda S, Solo K, Yaacoub S, Schünemann HJ, et al. Physical distancing, face masks, and eye protection to prevent person-to-person transmission of SARS-CoV-2 and COVID-19: a systematic review and meta-analysis. Lancet. 2020;395:1973-87. https://doi.org/10.1016/S0140-6736(20)31142-9
Singanayagam A, Patel M, Charlett A, Lopez Bernal J, Saliba V, Ellis J, et al. Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England. Euro Surveill. 2020;25:2001483. https://doi.org/10.2807/1560-7917.ES.2020.25.32.2001483
Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25:2000045. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045
Centers for Disease Control and Prevention. Duration of isolation and precautions for adults with COVID-19. Atlanta: CDC; 2020.
Binnicker MJ. Can the severe acute respiratory syndrome coronavirus 2 polymerase chain reaction cycle threshold value and time from symptom onset to testing predict infectivity? Clin Infect Dis. 2020;735. https://doi.org/10.1093/cid/ciaa735
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