Identification and differential expression of the microphthalmia-associated transcription factor in heart and isolated cardiomyocytes from Guinea pigs: Possible role in hypertrophy and viability
Abstract
Introduction: The microphthalmia-associated transcription factor (MITF) regulates the expression of specific genes and its cardiac expression and function is not known.
Objectives: To identify the expression of MITF in hearts and isolated cardiomyocytes from Guinea pigs, to describe morphological changes associated with mRNA interference of MITF and to evaluate their relative changes in expression in isolated cardiomyocytes under ischemic preconditioning.
Materials and methods: The cardiac specific isoform, MITF-H, and relative expression level analysis, was determined by semi-quantitative real time PCR, sequencing and Western blotting. Reduction of mRNA-MITF-H was induced by transduction of specific-MITF-shRNAi interference. The cardiac morphological changes, diameter and number of cardiac fibers were evaluated by direct observation and light microscopy.
Results: A cDNA fragment of 281 bp was amplified from heart and isolated ventricular cardiac myocytes. Sequence analysis confirmed the identity of the isoform MITF-H, exon 1. The MITF silencing was associated with an increase in cardiac index (heart weight/body weight vs. 5.46 x 10-3 vs 4.6 x 10-3) and higher diameter of cardiac fibers (50.2±16 μm vs 38,7±14,7 μm p<0.05, n=150). In isolated cardiac myocytes under ischemic preconditioning we observed a higher relative expression compared with that measured in myocytes exposed to normoxia and simulated ischemia (eighty and one hundred times, p <0.05, n = 5).
Conclusion. The results suggest that MITF-H isoform may be involved in Guinea pig cardiac hypertrophy, response to stress by ischemia and cardiomyocytes survival.
Downloads
Some similar items:
- María Clara Echeverry, Nubia Catalina Tovar, Guillermo Mora, Presence of antibodies to cardiac neuroreceptors in patients with Chagas disease , Biomedica: Vol. 29 No. 3 (2009)
- Ismael Reyes, Raj Tiwari, Jan Geliebter, Niradiz Reyes, DNA microarray analysis reveals metastasis-associated genes in rat prostate cancer cell lines , Biomedica: Vol. 27 No. 2 (2007)
- Lauro Figueroa-Valverde, Francisco Díaz-Cedillo, María López-Ramos, Elodia García-Cervera, Karen Quijano-Ascencio, Inotropic activity induced by carbamazepine-alkyne derivative in an isolated heart model and perfused to constant flow , Biomedica: Vol. 31 No. 2 (2011)
- Tania M. Cortázar, John Walker, Genetic manipulation and the study of the protozoan parasite Leishmania. , Biomedica: Vol. 24 No. 4 (2004)
- Jesús Orlando Yepes, María Luz Gunturiz, Luis Felipe Henao, María Cristina Navas, Norman Balcázar, Luis Alberto Gómez, Differential display of messenger RNA and identification of selenocysteine lyase gene in hepatocellular carcinoma cells transiently expressing hepatitis C virus core protein. , Biomedica: Vol. 26 No. 2 (2006)
- Tania Yadira Martínez-Rodríguez, Mauricio Rey-Buitrago, Alpha sinuclein expression in blood and its relationship with chronic constipation in a population from Bogotá, D.C., with problems of alcohol consumption , Biomedica: Vol. 40 No. 2 (2020)
- Concepción Sánchez-Martínez , Liliana Torres-González , Gabriela Alarcón-Galván , Linda E. Muñoz-Espinosa , Homero Zapata-Chavira , Diana Patricia Moreno-Peña, Homero Náñez-Terreros, Edelmiro Pérez-Rodríguez , Lourdes Garza-Ocañas , Francisco Javier Guzmán-de la Garza , Paula Andrea Cordero, Anti-inflammatory and antioxidant activity of essential amino acid α-ketoacid analogues against renal ischemia–reperfusion damage in Wistar rats , Biomedica: Vol. 40 No. 2 (2020)
- Omid Jazayeri, Setareh Farahmand Araghi, Tahereh A. Aghajanzadeh, Fereshteh Mir Moammadrezaei, Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes , Biomedica: Vol. 41 No. 4 (2021)
Article metrics | |
---|---|
Abstract views | |
Galley vies | |
PDF Views | |
HTML views | |
Other views |