Characterization by pulsed-field gel electrophoresis of Salmonella Typhimurium isolates recovered in the acute diarrheal disease surveillance program in Colombia, 1997-2004
Keywords:
Salmonella Typhimurium, epidemiologic surveillance, diarrhea, electrophoresis, gel, pulsed-field, Colombia
Abstract
Introduction. In Colombia Salmonella Typhimurium is the second most frequently isolated Salmonella serotype (after S. Enteritidis) from human clinical samples. This serotype represents 28.2% (n = 468) of the 1659 Salmonella isolates recovered by the Instituto Nacional de Salud (INS) Acute Diarrheal Diseases Surveillance Program from 1997 to 2004. Previously, little was known about the molecular attributes of these strains and commonality of genetic subtypes among S. Typhimurium isolated from humans in Colombia.Objective. The purpose of this study was to characterize S. Typhimurium isolates circulating in Colombia using pulsed-field gel electrophoresis in order to establish genetic profiles and determine their potential relatedness.
Materials and methods. 468 S. Typhimurium isolates were submitted to INS by 14 Public Health Laboratories from January 1997 to December 2004. The pulsed-field gel electrophoresis was performed using restriction enzyme XbaI according to the PulseNet standard protocol (CDC, Atlanta). Restriction pattern analysis and cluster definition were performed using Molecular Analyst Fingerprinting II software.
Results. 180 distinct electrophoretic patterns were obtained. Pattern COINJPX.X01.0062 was the most common (21.3% of the 473 isolates, n=101), followed by COINJPX.X01.0001 (8.4%, n=40), COINJPX.X01.0058 (3%, n=13), COINJPX.X01.0003 (2.5%, n=12), COINJPX.X01.0066 (2.3%, n=11), and COINJPX.X01.0108 (1.4%, n=6). COINJPX.X01.0001, was the most prevalent pattern among S. Typhimurium isolated in Bogotá from 1997-2002, but appeared to be replaced both in Bogotá and nationally by COINJPX.X01.0062 in 2003-2004. Cluster analysis demonstrated three distinct groups with 53% and 82% similarity indicating heterogeneity within the serotype.
Conclusions. The study provides important electrophoretic pattern baseline data, which will be useful for prospective real-time comparison of profiles of future isolates. Cluster detection using this method will facilitate outbreak detection and will enhance our ability to find common sources for seemingly unrelated infections.
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References
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21. Wiesner M, Hidalgo M, Castañeda E, Agudelo CI. Molecular analysis of Salmonella Enteritidis and Typhimurium clinical and food isolates by Pulse-Field Gel Electrophoresis in Bogotá, Colombia. Microb Drug Resistant 2006;12:68-74.
22. Zhao S, Fedorka-Cray PJ, Friedman S, McDermott PF, Walter RD, Qaiyumi S, et al. Characterization of Salmonella Typhimurium origin obtained from the National Antimicrobial Resistance Monitoring System. Foodborne Pathog Dis 2005;2:169-81.
23. Guerra B, Schrors P, Mendoza MC. Application of PFGE performed with XbaI to an epidemiological and phylogenetic study of Salmonella serotype Typhimurium. Relations between genetic types and phage types. New Microbiol 2000;23:11-20.
24. Tsen HY, Lin JS, Hsih HY. Pulse field electrophoresis for animal Salmonella enterica serovar Typhimurium isolates in Taiwan. Vet Microbiol 2002;87:73-80.
25. On SL, Baggesen DL. Determination of clonal relationships of Salmonella Typhimurium by numerical analysis of macrorestriction profiles. J Appl Microbiol 1997;83:699-706.
26. Fakhr MK, Nolan LK, Logue CM. Multilocus sequence typing lacks the discriminatory ability of pulsed-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium. J Clin Microbiol 2005;43:2215-9.
27. Oshibe T, Hamada K. Phylogenetic analysis of genotypic variations of Salmonella Enteritidis isolates from sporadic infections using pulsed field gel electrophoresis from December 1996 to November 2000 in Hyogo Prefecture. Jpn J Infect Dis 2001;54:40-3.
28. Sulakvelidze A, Kekelidze M, Turabelidze D, Tsanava S, Tevsadze L, Devdariani L, et al. Salmonellosis in the republic of Georgia: using molecular typing to identify the outbreak-causing strain. Emerg Infect Dis 2000;6:70-3.
29. Refsum T, Heir E, Kapperud G, Vardund T, Holstad T. Molecular epidemiology of Salmonella enterica serovar Typhimurium isolates determined by pulsedfield gel electrophoresis: comparison of isolates from avian wildlife, domestic animals and the environment in Norway. Appl Environ Microbiol 2002;68:5600-6.
2. Jaramillo E, Estrada S, Ospina S. Etiología de la enfermedad diarreica aguda (EDA) de origen bacteriano, utilizando un protocolo estandarizado de laboratorio. Infectio 1999;3:95-9.
3. Popoff MY. Antigenic formulas of the Salmonella serovars. 8th revision. Paris: Pasteur Institute, World Health Organization Collaborating Centre for Reference and Research on Salmonella; 2001.
4. Wang L, Andrianopoulos K, Liu D, Popoff MY, Reeves PR. Extensive variation in the O-antigen gene cluster within one Salmonella enterica serogroup reveals an unexpected complex history. J Bacteriol
2002;184:1669-77.
5. Olsen SJ, Bishop R, Brenner FW, Roels TH, Bean N, Tauxe RV, et al. The changing epidemiology of Salmonella: trends in serotypes isolated from humans in the United States, 1987-1997. J Infect Dis
2001;183:753-61.
6. CDC Salmonella surveillance. Annual summary, 2002. Atlanta, GE: US Deparment of Health and Human Services, CDC; 2003.
7. Organización Panamericana de la Salud. Reunión anual regional de los países participantes en la red de monitoreo de vigilancia de la resistencia a los antibióticos. Asunción, Paraguay, 31 Enero - 2 Febrero, 2001. [Consultado: 3 de marzo de 2006] OPS HCP/HCT/201/02. http://www.paho.org/Spanish/AD/DPC/CD/amr-resultados-2000.htm
8. Grupo de Microbiología, Instituto Nacional de Salud. Distribución de los aislamientos de Salmonella spp. por procedencia y por año 1997-2003. En Vigilancia de serotipos y patrones de susceptibilidad antimicrobiana de los patógenos de importancia en salud pública. Agudelo CI. [Consultado: 3 de marzo de 2006] Disponible: http://www.ins.gov.co/pdf_investiga/Microbiologia_salm_05.pdf
9. Guzmán-Blanco M, Casellas JM, Sader HS. Bacterial resistance to antimicrobial agents in Latin America. The giant is awakening. Infecf Dis Clin North Am 2000;14:67-81.
10. Bender JB, Hedberg CW, Boxrud DJ, Besser JM, Wicklund JH, Smith KE, et al. Use of molecular subtyping in surveillance for Salmonella enterica serotype Typhimurium. N Engl J Med 2001;344:189-
95.
11. Lindstedt BA, Heir E, Gjernes E, Kapperud G. DNA fingerprinting of Salmonella enterica subsp. enterica serovar Typhimurium with emphasis on phage type DT104 based on variable number of tandem repeat loci. J Clin Microbiol 2003;41:1469-79.
12. Lailler R, Grimont F, Jones Y, Sanders P, Brisabois A. Subtyping of Salmonella Typhimurium by pulsedfield gel electrophoresis and comparisons with phage types and resistance types. Pathol Biol 2002;50:361-8.
13. Tamada Y, Nakaoka Y, Nishimori K, Doi A, Kumaki T, Uemura N, et al. Molecular typing and epidemiological study of Salmonella enterica serotype Typhimurium isolates from cattle by fluorescent amplified-fragment length polymorphism fingerprinting and pulsed-field gel electrophoresis. J Clin Microbiol 2001;39:1057-66.
14. Swaminathan B, Barret TJ, Hunter SB, Tauxe RV, CDC PulseNet Task Force. PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg Infect Dis
2001;7:382-9.
15. Soto S, Guerra B, Gonzáles M, Mendoza M. Potencial of three way randomly amplified polimorphic DNA analysis as a typing method for twelve Salmonella serotypes. Appl Environ Microbiol 1999;65:4830-6.
16. CDC, APHL. PulseNet. Atlanta, GA: U.S. Department of Health and Human Services, CDC; 2004. [Consultado 3 de marzo de 2006]. Disponible en: www.cdc.gov/pulsenet.
17. Red PulseNet América Latina. Pulsenet America Latina - Plan de Acción - 2004-2005. [Consultado: 3 de abril de 2006]. Disponible en: http://www.panalimentos.org/pulsenet/files/7773553729_PULSENET-Plan%20de%20Accion-22-05-04.doc.
18. Hidalgo M, Gracia M, Ovalle M, Wiesner M, Chávez J, Realpe ME, et al. Caracterización de los aislamientos de Salmonella Typhimurium, asociados con un brote de intoxicación alimentaria en una localidad de Pasto, Nariño. Inf Quinc Epidemiol Nac 2004;9:89-92.
19. Bopp CA, Brenner FW, Fields PI, Wells JG, Srockbine NA. Escherichia, Shigella and Salmonella. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, editors. Manual of clinical microbiology. 8th ed. Washington, DC: American Society for Microbiology Press; 2003.p.654-83.
20. Hunter SB, Vauterin P, Lambert-Fair MA, Van Duyne S, Kubota K, Graves L, et al. Establishment of a universal size standard strain for use with the pulseNet standardize pulse-field gel electrophoresis protocols: converting the national data bases to the new size standard. J Clin Microbiol 2005;43:1045-50.
21. Wiesner M, Hidalgo M, Castañeda E, Agudelo CI. Molecular analysis of Salmonella Enteritidis and Typhimurium clinical and food isolates by Pulse-Field Gel Electrophoresis in Bogotá, Colombia. Microb Drug Resistant 2006;12:68-74.
22. Zhao S, Fedorka-Cray PJ, Friedman S, McDermott PF, Walter RD, Qaiyumi S, et al. Characterization of Salmonella Typhimurium origin obtained from the National Antimicrobial Resistance Monitoring System. Foodborne Pathog Dis 2005;2:169-81.
23. Guerra B, Schrors P, Mendoza MC. Application of PFGE performed with XbaI to an epidemiological and phylogenetic study of Salmonella serotype Typhimurium. Relations between genetic types and phage types. New Microbiol 2000;23:11-20.
24. Tsen HY, Lin JS, Hsih HY. Pulse field electrophoresis for animal Salmonella enterica serovar Typhimurium isolates in Taiwan. Vet Microbiol 2002;87:73-80.
25. On SL, Baggesen DL. Determination of clonal relationships of Salmonella Typhimurium by numerical analysis of macrorestriction profiles. J Appl Microbiol 1997;83:699-706.
26. Fakhr MK, Nolan LK, Logue CM. Multilocus sequence typing lacks the discriminatory ability of pulsed-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium. J Clin Microbiol 2005;43:2215-9.
27. Oshibe T, Hamada K. Phylogenetic analysis of genotypic variations of Salmonella Enteritidis isolates from sporadic infections using pulsed field gel electrophoresis from December 1996 to November 2000 in Hyogo Prefecture. Jpn J Infect Dis 2001;54:40-3.
28. Sulakvelidze A, Kekelidze M, Turabelidze D, Tsanava S, Tevsadze L, Devdariani L, et al. Salmonellosis in the republic of Georgia: using molecular typing to identify the outbreak-causing strain. Emerg Infect Dis 2000;6:70-3.
29. Refsum T, Heir E, Kapperud G, Vardund T, Holstad T. Molecular epidemiology of Salmonella enterica serovar Typhimurium isolates determined by pulsedfield gel electrophoresis: comparison of isolates from avian wildlife, domestic animals and the environment in Norway. Appl Environ Microbiol 2002;68:5600-6.
How to Cite
1.
Muñoz N, Realpe ME, Castañeda E, Agudelo CI. Characterization by pulsed-field gel electrophoresis of Salmonella Typhimurium isolates recovered in the acute diarrheal disease surveillance program in Colombia, 1997-2004. biomedica [Internet]. 2006 Sep. 1 [cited 2024 May 18];26(3):397-40. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/358
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