The Importance of investigating Mycobacterium bovis in clinical samples of human origin
Abstract
Introduction: Tuberculosis is an infectious disease that still represents a major public health problem worldwide. It is one of the main causes of mortality in people with HIV.
Objective: To identify the presence of M. bovis as an etiological agent of human tuberculosis in sputum smear positive samples using the test Genotype MTBC™.
Materials and methods: We conducted a descriptive study, 88 sputum samples were submitted to the Grupo de Micobacterias of the Instituto Nacional de Salud between January and November, 2015. We used the conventional microbiological analysis and the molecular test Genotype MTBC™ to identify the M. tuberculosis complex species.
Results: Sixty two (70.5%) were males; the most affected groups were those between 24 and 34 years old, those residing in the municipal seats and those affiliated to the subsidized health plans. In 50.0% (44) of the samples with a result in the species identification test, we detected M. tuberculosis.
Conclusion: The highest burden of the disease was recorded among the male population in productive ages. The identification test for species of the complex showed all were M. tuberculosis. However, it is not possible to discard the presence of M. bovis in humans in Colombia. The differential identification of species should be done in risk groups and in areas where the circulation of this mycobacterium in cattle is known.
Downloads
References
Tortoli E. Microbiological features and clinical relevance of new species of the genus Mycobacterium. Clin Microbiol Rev. 2014;27:727-52. https://doi:10.1128/CMR.00035-14
Ramos DF, Silva AB, Fagundes MQ, von Groll A, Almeida PE, Dellagostin OA. Molecular typing of Mycobacterium bovis isolated in the south of Brazil. Braz J Microbiol. 2014;45:657-60. https://doi.org/10.1590/S1517-83822014000200039
World Health Organization. Global Tuberculosis Report, 2016. Fecha de consulta: 5 de diciembre de 2017. Disponible en: http://apps.who.int/medicinedocs/documents/s23098en/s23098en.pdf
Zumla A, Granje J. Doing something about tuberculosis. BMJ. 1999;318:956-8. https://doi.org/10.1136/bmj.318.7189.956
Instituto Nacional de Salud. Informe del evento preliminar de tuberculosis, hasta el periodo epidemiológico XIII, Colombia, 2017. Fecha de consulta: 18 de abril de 2018. Disponible en: http://www.ins.gov.co/buscador-eventos/Informesdeevento/TUBERCULOSIS%20PE%20XIII%202017.pdf
Cosivi O, Grange JM, Daborn CJ, Raviglione MC, Fujikura T, Cousins D, et al. Zoonotic tuberculosis due to Mycobacterium bovis in developing countries. Emerg Infect Dis. 1998;4:59-70. https://doi.org/10.3201/eid0401.980108
Torres P, Soberanis O, Martínez A, Chávez B, Barrios MT, Torres M, et al. Prevalence of latent and active tuberculosis among dairy farm workers exposed to cattle infected by Mycobacterium bovis. PLoS Negl Trop Dis. 2013;7:1-8. https://doi.org/10.1371/journal.pntd.0002177
Üller B, Durr S, Alonso S, Hattendorf J, Laisse C, Parsons S, et al. Zoonotic Mycobacterium bovis induced tuberculosis in humans. Emerg Infect Dis. 2013;19:899-908. https://doi.org/10.3201/eid1906.120543
Abalos P, Retamal P. Tuberculosis: ¿una zoonosis re-emergente? Rev Sci Tech OffInt Epiz. 2004;23:583-94.
Pérez L, Navarro Y, García de Viedma D. Current knowledge and pending challenges in zoonosis caused by Mycobacterium bovis: A review. Res Vet Sci. 2014;97:S94-S100. https://doi.org/10.1016/j.rvsc.2013.11.008
Kantor IN, Ambroggib M, Poggib S, Morcilloc N, Da Silva MA, Osorio M, et al. Human Mycobacterium bovis infection in ten Latin American countries. Tuberculosis. 2008 88:358-65. https://doi.org/10.1016/j.tube.2007.11.007
Pérez L, Milian F, Arriaga C, Romero C, Escartin M. Epidemiología molecular de la tuberculosis bovina y humana en una zona endémica de Querétaro, México. Salud Pública Mex. 2008;50:286-91.
Kantor IN, LoBue PA, Thoen CO. Human tuberculosis caused by Mycobacterium bovis in the United States, Latin America and the Caribbean. Int J Tuberc Lung. 2010;14:1369-73.
Rivas C, Greif G, Coitinho C, Araújo L, Laserra P, Robello C. Primeros casos de tuberculosis pulmonar por Mycobacterium bovis. Una zoonosis reemergente en Uruguay. Rev Med Urug. 2012;28:209-14.
Park D, Qin H, Jain S, Preziosi M, Minuto J, Mathews C, et al. Tuberculosis due to Mycobacterium bovis patients coinfected with human inmunodeficiency virus. Clin Infect Dis. 2010;51:1343-6. https://doi.org/10.1086/657118
Hlavsa M, Moonan P, Cowan L, Navin T, Kammerer S, Morlock G, et al. Human tuberculosis due to Mycobacterium bovis in the United States, 1995-2005. Clin Infect Dis. 2008;47:168-75. https://doi.org/10.1086/589240
Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 1997;35:907-14.
Aranaz A, Liébana E, Mateos A, Domínguez L, Vidal D, Domingo M, et al. Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: A tool for studying epidemiology of tuberculosis. J Clin Microbiol 1996;34:2734-40.
Organización Panamericana de la Salud. Manual para el diagnóstico bacteriológico de la tuberculosis. Normas y guía técnica. Cultivo. Fecha de consulta: 5 de diciembre de 2017.Disponible en: http://www.sld.cu/galerias/pdf/sitios/tuberculosis/tb-labs-cultivo[2].pdf
Organización Panamericana de la Salud. Manual para el diagnóstico bacteriológico de la tuberculosis. Normas y guía técnica. Baciloscopia. Fecha de consulta: 5 de diciembre de 2017. Disponible en: http://www.sld.cu/galerias/pdf/sitios/tuberculosis/tb-labs-baciloscopia[1].pdf
Hain Lifescience GmbH. Insertotécnica Genotype MTBC™ para la diferenciación de especies del complejo M. tuberculosis. Nehren, Alemania: Hain Lifescience GmbH; 2012.
Federación Colombiana de Ganaderos. Tuberculosis bovina. Fecha de consulta: 20 de abril de 2018. Disponible en: http://www.fedegan.org.co/programas/tuberculosis-bovina
Instituto Colombiano Agropecuario. Boletín epidemiológico anual, Colombia sanidad animal, 2014. Fecha de consulta: 20 de abril de 2018. Disponible en: https://www.ica.gov.co/Areas/Pecuaria/Servicios/Epidemiologia-Veterinaria/Bol/Epi/Boletines-Anuales.aspx
Ministerio de Salud y Protección Social. Programa Nacional de Prevención y Control de la Tuberculosis. Plan de monitoreo y evaluación. Fecha de consulta: 22 de diciembre de 2017. Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/plan-monitoreo-evaluacion-tuberculosis.pdf
León CI, Sierra C, Naranjo N, Garzón MC, Guerrero MI. Segundo estudio nacional de resistencia primaria de M. tuberculosis a las drogas antituberculosas en Colombia. Infectio. 2002;6:83.
Garzón MC, Angee DY, Llerena C, Orjuela D, Victoria J. Vigilancia de la resistencia de Mycobacterium tuberculosis a los fármacos antituberculosos, Colombia 2004-2005. Biomédica. 2008;28:319-26. https://doi.org/10.7705/biomedica.v28i3.71
Puerto G, Erazo L, Wintaco M, Castro C, Ribón W, Guerrero MI. Mycobacterium tuberculosis genotypes determined by spoligotyping to be circulating in Colombia between 1999 and 2012 and their possible associations with transmission and susceptibility to firstline drugs. PLoS One. 2015;10:e0124308. https://doi.org/10.1371/journal.pone.0124308
Leal AF, Castro CM, Wintaco LM, Villalobos R, Puerto GM. Tuberculosis por Mycobacterium bovis en trabajadores de fincas en saneamiento para tuberculosis bovina, de Antioquia, Boyacá y Cundinamarca. Rev Salud Pública. 2016;18:727-37. https://doi.org/10.15446/rsap.v18n5.51187
Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35:907-14.
Arráiz N, Romay Z, Faría N, Mujica D. Identificación diferencial de aislados clínicos de Mycobacterium tuberculosis y Mycobacterium bovis por un ensayo de PCR múltiple. Rev Cient. 2006;16:622-8.
Some similar items:
- John-Leonardo Torres-Castiblanco, Jorge Alberto Carrillo, Daniel Hincapié-Urrego, Adriana Rojas-Villarraga, Tuberculosis in the era of anti-TNF-alpha therapy: Why does the risk still exist? , Biomedica: Vol. 38 No. 1 (2018)
- Claudia Llerena, Angie Zabaleta, Angélica Valbuena, Martha Murcia, Prevalence of Mycobacterium tuberculosis resistance to quinolones and injectables in Colombia, 2012-2013 , Biomedica: Vol. 37 No. 1 (2017)
- Leandro Galvis, Ángel Y. Sánchez, Leonardo F. Jurado, Martha I. Murcia, Tuberculosis associated with tumor necrosis factor-α antagonists, case description and analysis of reported cases in Colombia , Biomedica: Vol. 38 No. 1 (2018)
- Juan Gabriel Bueno-Sánchez, Jairo René Martínez-Morales, Elena E. Stashenko, Wellman Ribón, Anti-tubercular activity of eleven aromatic and medicinal plants occurring in Colombia , Biomedica: Vol. 29 No. 1 (2009)
- Diego Chaves, Andrea Sandoval, Luis Rodríguez, Juan C. García, Silvia Restrepo, María Mercedes Zambrano, Comparative analysis of six Mycobacterium tuberculosis complex genomes , Biomedica: Vol. 30 No. 1 (2010)
- Edna Catering Rodríguez, Sandra Yamile Saavedra, Aura Lucía Leal, Carlos Álvarez, Narda Olarte, Alberto Valderrama, Sonia Isabel Cuervo, Javier Escobar, The spread of KPC-3 Klebsiella pneumoniae in hospitals in Bogotá over a three-year period (2008-2010) , Biomedica: Vol. 34 (2014): Abril, Suplemento 1, Resistencia bacteriana
- Diana Castaño, Mauricio Rojas, Alterations in recruitment and activation of Rab proteins during mycobacterial infection , Biomedica: Vol. 30 No. 2 (2010)
- Ronald Maestre-Serrano, Suljey Cochero, Betsy Bello, Cristina Ferro, Registry and distribution update of the species of the Haemagogus (Diptera: Culicidae) genus in the Caribbean region of Colombia , Biomedica: Vol. 33 (2013): Suplemento 1, Fiebres hemorrágicas
- Myriam Consuelo López, Ligia Inés Moncada, Yoseth Ariza-Araújo, Julián Alfredo Fernández-Niño, Patricia Reyes, Rubén Santiago Nicholls, Evaluation (assessment) of three tests for diagnosis of geohelmints in Colombia , Biomedica: Vol. 33 No. 1 (2013)
- Adriana Rojas-Villarraga, Carlos Andrés Agudelo, Ricardo Pineda-Tamayo, Alvaro Porras, Gustavo Matute, Juan Manuel Anaya, Tuberculosis in patientes treated with tumor necrosis factor alpha antagonists living in an endemic area. Is the risk worthwhile? , Biomedica: Vol. 27 No. 2 (2007)
| Article metrics | |
|---|---|
| Abstract views | |
| Galley vies | |
| PDF Views | |
| HTML views | |
| Other views | |
