Association of VDR gene ApaI polymorphism with obesity in Iranian population
Abstract
Introduction: Identifying obesity risk factors as a health problem facing communities is crucial given its complexity. The vitamin D receptor gene has been reported as a possible cause of this disease.
Objective: To study the association of the VDR gene ApaI, BsmI, and TaqI polymorphisms with obesity in an Iranian population.
Material and methods: We analyzed the genotypes of 348 obese (BMI≥30 kg/m2) and 320 non-obese people (BMI: 18.5-24.9 kg/m2) using PCR-RFLP. We measured FBS, TG, total cholesterol, and HDL and LDL cholesterol levels in an automatic biochemical analyzer.
Results: We found significantly higher BMI, FBS, and TG levels in the obese group compared to the control. In the obese individuals, the frequency of genotype AA was 47.1% and that of the combined Aa+aa genotype, 52.9% while in the control group they were 30% and 70%, respectively (p=0.024, 95% confidence interval (CI)=1.100-3.933, odds ratio (OR)=2.08). A and a alleles frequencies for the ApaI polymorphism were statistically significant in the two groups (allele A vs. a; p=0.017). No significant relationship was observed between TaqI genotypes and alleles in the control and obese subjects.
Conclusion: We found that VDR ApaI (rs7975232 C/A) polymorphism appeared to be a risk factor for obesity. Especially, the A allele and the AA genotype in ApaI were associated with the obesity phenotypes.
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References
Walley AJ, Asher JE, Froguel P. The genetic contribution to non-syndromic human obesity. Nat Rev Genet. 2009;10:431-42. https://doi.org/10.1038/nrg2594
Kopelman P. Health risks associated with overweight and obesity. Obes Rev. 2007;8(Suppl.1):13-17. https://doi.org/10.1111/j.1467-789X.2007.00311.x
World Health Organization W. Obesity: Preventing and managing the global epidemic. WHO Technical Report Series 894. 894th edition. Geneva: WHO; 2000.
Blum M, Dolnikowski G, Seyoum E, Harris SS, Booth SL, Petersonet J, et al. Vitamin D (3) in fat tissue. Endocrine. 2008;33:90-4. https://doi.org/10.1007/s12020-008-9051-4
Hypponen E, Power C. Vitamin D status and glucose homeostasis in the 1958 British birth cohort: The role of obesity. Diabetes Care. 2006;29:2244-6. https://doi.org/10.2337/dc06-0946
Wortsman J, Matsuoka LY, Chen TC, Lu Z, Holick MF. Decreased bioavailability of vitamin D in obesity. Am J Clin Nutr. 2000;72:690-3. https://doi.org/10.1093/ajcn/72.3.690
Uitterlinden AG, Fang Y, van Meurs JB, Pols HA, van Leeuwen JP. Genetics and biology of vitamin D receptor polymorphisms. Gene. 2004;338:143-56. https://doi.org/10.1016/j.gene.2004.05.014
Tabrizi JS, Sadeghi-Bazargani H, Farahbakhsh M, Nikniaz L, Nikniaz Z. Prevalence and associated factors of overweight or obesity and abdominal obesity in Iranian population: A population-based study of Northwestern Iran. Iran J Public Health. 2018;47:1583-92.
Mirzazadeh A, Sadeghirad B, Haghdoost AA, Bahreini F, Rezazadeh Kermani M. The prevalence of obesity in Iran in recent decade; a systematic review and meta-analysis study. Iran J Public Health. 2009; 38: 1-11.
Faghfouri AH, Faghfuri E, Maleki V, Payahoo L, Balmoral A, Khaje Bishak Y. A comprehensive insight into the potential roles of VDR gene polymorphism in obesity: A systematic review. Arch Physiol Biochem. 2020;1-13. https://doi.org/10.1080/13813455.2020.1788097
Vasilopoulos Y, Sarafidou T, Kotsa K, Papadimitriou M, Goutzelas Y, Stamatis C, et al. VDR TaqI is associated with obesity in the Greek population. Gene. 2013;512:237-9. https://doi.org/10.1016/j.gene.2012.10.044
Chen X, Wang W, Wang Y, Han X, Gao L. Vitamin D receptor polymorphisms associated with susceptibility to obesity: A meta-analysis. Med Sci Monit. 2019;25:8297-305. https://doi.org/10.12659/MSM.915678
World Health Organization. Obesity and overweight. 2013. Available from: https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight
Meamar R, Javadirad SM, Chitsaz N, Asadian M, Kazemi M, Ostadsharif M. Vitamin D receptor gene variants in Parkinson’s disease patients. Egypt J Med Hum Genet. 2017;18:225-30. https://doi.org/10.1016/j.ejmhg.2016.08.004
Khammissa RA, Fourie J, Motswaledi MH, Ballyram R, Lemmer J, Feller L. The biological activities of vitamin D and its receptor in relation to calcium and bone homeostasis, cancer, immune and cardiovascular systems, skin biology, and oral health. Biomed Res Int. 2018;2018. https://doi.org/10.1155/2018/9276380
Fleet JC. The role of vitamin D in the endocrinology controlling calcium homeostasis. Mol Cell Endocrinol. 2017;15:36-45. https://doi.org/10.1016/j.mce.2017.04.008
Bid HK, Mishra DK, Mittal RD. Vitamin-D receptor (VDR) gene (Fok-I, Taq-I and Apa-I) polymorphisms in healthy individuals from north Indian population. Asian Pac J Cancer Prev. 2005;6:147-52.
Zmuda JM, Cauley JA, Ferrell RE. Molecular epidemiology of vitamin D receptor gene variants. Epidemiol Rev. 2000;22:203-17. https://doi.org/10.1093/oxfordjournals.epirev.a018033
Ogunkolade BW, Boucher BJ, Prahl JM, Bustin SA, Burrin JM, Noonan K, et al. Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians. Diabetes. 2002;51:2294-300. https://doi.org/10.2337/diabetes.51.7.2294
Al-Daghri NM, Guerini FR, Al-Attas OS, Alokail MS, Alkharfy KM, et al. Vitamin D receptor gene polymorphisms are associated with obesity and inflammosome activity. PLoS ONE. 2014;9:e102141. https://doi.org/10.1371/journal.pone.0102141
Rahmadhani R, Zaharan NL, Mohamed Z, Moy FM, Jalaludin MY. The associations between VDR BsmI polymorphisms and risk of vitamin D deficiency, obesity and insulin resistance in adolescents residing in a tropical country. PLoS ONE. 2017;12:e0178695. https://doi.org/10.1371/journal.pone.0178695
Ferrarezi DAF, Bellili-Munoz N, Nicolau C, Cheurfa N, Guazzelli IC, Frazzatto E, et al. Allelic variations in the vitamin D receptor gene, insulin secretion and parents’ heights are independently associated with height in obese children and adolescents. Metabolism. 2012;61:1413-21. https://doi.org/10.1016/j.metabol.2012.03.018
Correa-Rodríguez M, Carrillo-Ávila JA, Schmidt-RioValle J, González-Jiménez E, Vargas S, Martín J, et al. Genetic association analysis of vitamin D receptor gene polymorphisms and obesity-related phenotypes. Gene. 2018;15:51-6. https://doi.org/10.1016/j.gene.2017.10.029
Al-Hazmi AS, Al-Mehmadi MM, Al-Bogami SM, Shami AA, Al-Askary AA, Alomery AM, et al. Vitamin D receptor gene polymorphisms as a risk factor for obesity in Saudi men. Electronic Physician. 2017;9:5427-33. https://doi.org/10.19082/5427
Ostadsharif M, Rashidi-Khorasgani F. The relationship of FokI polymorphism in vitamin D receptor (VDR) gene and obesity. J Isfahan Med Sch. 2015;33:350.
Jakubowska-Pietkiewicz E, Klich I, Fendler W, Mlynarski W, Chlebna-Sokol D. Effect of vitamin D receptor gene (VDR) polymorphism on body height in children-own experience. Postepy Hig Med Dosw (Online). 2013;67:873-8. https://doi.org/10.5604/17322693.1063747
Nosratabadi R, Arababadi MK, Salehabad VA, Shamsizadeh A, Mahmoodi M, Sayadi AR, et al. Polymorphisms within exon 9 but not intron 8 of the vitamin D receptor are associated with the nephropathic complication of type-2 diabetes. Int J Immunogenet. 2010;37:493-7. https://doi.org/10.1111/j.1744-313X.2010.00953.x
Tokita A, Matsumoto H, Morrison NA, Tawa T, Miura Y, Fukamauchi K, et al. Vitamin D receptor alleles, bone mineral density and turnover in premenopausal Japanese women. J Bone Miner Res. 1996;11:1003-9. https://doi.org/10.1002/jbmr.5650110718
Fountas L, Moutsatsou P, Kastanias I, Tamouridis N, Tzanela M, Anapliotou M, et al. The contribution of vitamin d receptor gene polymorphisms in osteoporosis and familial osteoporosis. Osteoporos Int. 1999;10:392-8. https://doi.org/10.1007/s001980050245
Li L, Wu B, Liu J-Y, Yang L-B. Vitamin D receptor gene polymorphisms and type 2 diabetes: A meta-analysis. Arch Med Res. 2013;44:235-41. https://doi.org/10.1016/j.arcmed.2013.02.00
Kamel MM, Fouad SA, Salaheldin O, Abd El-Razek AA, Abd El-Fatah AI. Impact of vitamin D receptor gene polymorphisms in pathogenesis of type-1 diabetes mellitus. Int J Clin Exp Med. 2014;7:5505-10.
Fatma H, Abdul SN. Association of vVitamin D receptor gene BsmI polymorphism with type 2 diabetes mellitus in Pakistani population. Afri Health Sci. 2019;19 2164-71. https://doi.org/10.4314/ahs.v19i2.41
Sikander M. Relationship of ApaI VDR gene polymorphism to type 2 diabetes mellitus in Pakistani population. Electronic J Biol. 2017;13:338-42.
Santos BR, Lecke SB, Spritzer PM. Apa-I polymorphism in VDR gene is related to metabolic syndrome in polycystic ovary syndrome: A cross-sectional study. Reprod Biol Endocrinol. 2018;16:38. https://doi.org/10.1186/s12958-018-0355-9
Angel B, Lera L, Márquez C, Albala C. The association of VDR polymorphisms and type 2 diabetes in older people living in community in Santiago de Chile. Nutr Diabetes. 2018;8:31. https://doi.org/10.1038/s41387-018-0038-9
Mohamed S, El-Askary A. Vitamin D receptor gene polymorphism among Egyptian obese children. Asian J Clin Nutr. 2017;9:24-9. https://doi.org/10.3923/ajcn.2017.24.29
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