Differentiation by geometric morphometrics among 11 Anopheles (Nyssorhynchus) in Colombia
Abstract
Introduction. The correct identification of the Anopheles species of the subgenus Nyssorhynchus is important because this subgenus includes the main malaria vectors in Colombia. This information is necessary for focusing a malaria control program.Objective. Geometric morphometrics were used to evaluate morphometric variation of 11 species of subgenus Nyssorhynchus present in Colombia and to distinguish females of each species.
Materials and methods. The specimens were obtained from series and family broods from females collected with protected human hosts as attractants. The field collected specimens and their progeny were identified at each of the associated stages by conventional keys. For some species, wild females were used. Landmarks were selected on wings from digital pictures from 336 individuals, and digitized with coordinates. The coordinate matrix was processed by generalized Procrustes analysis which generated size and shape variables, free of non-biological variation. Size and shape variables were analyzed by univariate and multivariate statistics.
Results. The subdivision of subgenus Nyssorhynchus in sections is not correlated with wing shape. Discriminant analyses correctly classified 97% of females in the section Albimanus and 86% in the section Argyritarsis. In addition, these methodologies allowed the correct identification of 3 sympatric species from Putumayo which have been difficult to identify in the adult female stage.
Conclusion. The geometric morphometrics were demonstrated to be a very useful tool as an adjunct to taxonomy of females the use of this method is recommended in studies of the subgenus Nyssorhynchus in Colombia
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References
1. World Health Organization. Malaria. Fact Sheet Nº 94. Geneve: WHO; 2007. Consultado: marzo de 2008. Disponible en: http://www.who.int/mediacentre/factsheets/fs094/en/index.html.
2. Olano VA, Brochero LH, Saenz R, Quiñones ML, Molina JA. Mapas preliminares de la distribución de especies de Anopheles vectores de malaria en Colombia. Biomédica. 2001;21:402-8.
3. Quiñones ML, Ruiz F, Calle DA, Harbach RE, Erazo HF, Linton YM. Incrimination of Anopheles (Nyssorhynchus) rangeli and An. (Nys.) oswaldoi as natural vectors of Plasmodium vivax in Southern Colombia. Mem Inst Oswaldo Cruz. 2006;101:617-23.
4. Faran ME. Mosquito studies (Diptera: Culicidae). XXXIV. A revisions of the Albimanus Section of the subgenus Nyssorhynchus of Anopheles. Contrib Am Entomol Inst. 1980;15:1-215.
5. Faran ME, Linthicum KJ. A Handbook of the species of Anopheles (Nyssorhynchus) (Diptera: Culicidae). Mosq Syst. 1981;13:1-81.
6. Linthicum KJ. A revision of the Argyritarsis Section of the subgenus Nyssorhynchus of Anopheles (Diptera: Culicidae). Mosq Syst. 1988;20:99-271.
7. Suárez MF, Quiñones M, Fleming GA, Robayo M. Guía introductoria a la morfología de Anopheles y clave para determinación de las principales especies de Colombia. Bogotá, D.C.: Ministerio de Salud; 1988.
8. Quiñones ML, Harbach RE, Calle DA, Ruiz F, Erazo HF. Variante morfológica de Anopheles benarrochi (Diptera: Culicidae) en Putumayo, Colombia. Biomédica. 2001;21:351-9.
9. Frizzi G. Salivary gland chromosomes of Anopheles. Nature. 1947;160:226-8.
10. Frizzi G. Etude cytogenetique d´ Anopheles maculipennis en Italie. Bull World Health Organ. 1953;9:335-44.
11. Miles SJ. A biochemical key to adult members of the Anopheles gambiae group of species (Diptera: Culicidae). J Med Entomol. 1979;15:297-9.
12. Foley DH, Bryan JH. Electrophoretic keys to identify members of the Anopheles punctulatus complex of vector mosquitoes in Papua New Guinea. Med Vet Entomol. 1993;7:49-53.
13. Wilkerson RC, Parsons TJ, Klein TA, Gaffigan TV, Bergo E, Consolim J. Diagnosis by random amplified polymorphic DNA polymerase chain reaction of four cryptic species related to Anopheles albitarsis from Paraguay, Argentina and Brazil. J Med Entomol. 1995; 32:697-704.
14. Marelli MT, Malafronte RS, Florez-Mendoza C, Lourenco- de- Oliveira R, Kloetzel JK, Marinotti O. Sequence analysis of the second internal transcribed spacer of ribosomal DNA in Anopheles oswaldoi (Diptera: Culicidae). J Med Entomol. 1999;36:679-84.
15. Rohlf FJ, Marcus LF. A revolution in morphometrics. Trends Ecol Evol. 1993;8:129-32.
16. Matias A, De la Riva JX, Torrez M, Dujardin JP. Rhodnius robustus in Bolivia identified by its wings. Mem Inst Oswaldo Cruz. 2001;96:947-50.
17. Villegas J, Feliciangeli MD, Dujardin JP. Wing shape divergence between Rhodnius prolixus from Cojedes (Venezuela) and Rhodnius robustus from Mérida (Venezuela). Infect Genet Evol. 2002;2:121-8.
18. Gumiel L, Catala S, Noireau F, Rojas de Arias A, Garcia A, Dujardin JP. Wing geometry in Triatoma infestans (Klug) and T. melanosoma Martinez, Olmedo & Carcavallo (Hemiptera:Reduvidae). Syst Entomol 2003;28:173-9.
19. Dujardin JP. MOGwin (Software for Generalized Procrustes Analysis). Institut de Recherches pour le Développement. 2004. Consultado: marzo de 2008. Disponible en: http://www.mpl.ird.fr/morphometrics/mog/index.html
20. Dujardin JP. PADwin (Software for Discriminant Analysis). Institut de Recherches pour le Développement. 2004. Consultado: marzo de 2008. Disponible en: http://www.mpl.ird.fr/morphometrics/pad/
21. Calle DA, Quiñones ML, Erazo H, Jaramillo O. Morphometric discrimination of females of five species of Anopheles of the subgenus Nyssorhynchus from Southern and Northwest Colombia. Mem Inst Oswaldo Cruz. 2002;97:1191-5.
22. Delgado N, Rubio-Palis Y. Identification of Anopheles (Nyssorhynchus) (Diptera: Culicidae) occurring in Western Venezuela. Mosq Syst. 1993;25:222-30.
23. Rubio-Palis Y. Caracterización morfométrica de poblaciones de Anopheles (Nyssorhynchus) darlingi del sur de Venezuela. Bol Entomol Venez. 1998;13: 141-72.
24. Rubio-Palis Y. Anopheles (Nyssorhynchus) de Venezuela: taxonomía, bionomía, ecología e importancia médica. Maracay: Escuela de Malariología y Saneamiento Ambiental; 2000. p. 120.
25. Jirakanjanakit N, Dujardin JP. Discrimination of Aedes aegypti (Diptera: Culicidae) laboratory lines based on wing geometry. Southeast Asian J Trop Med Public Health. 2005;36:858-61.
26. Jirakanjanakit N, Leemingsawat S, Thongrungkiat S, Apiwathnasor C, Singhaniyom S, Bellec C, et al. Influence of larval density or food variation on the geometry of the wing of Aedes (Stegomyia) aegypti. Trop Med Int Health. 2007;12:1354-60.
27. Ruiz F, Quiñones ML, Erazo H, Calle DA, Alzate FA, Linton YM. Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and A. (N.) oswaldoi from Southern Colombia. Mem Inst Oswaldo Cruz. 2005;100:155-60.
28. Rohlf J. tpsDig, version 1.04. Stony Brook, NY: Department of Ecology and Evolution, State University of New York at Stony Brook; 2004. Consultado: marzo de 2008. Disponible en: http://life.bio.sunysb.edu/morph.
29. Rohlf FJ, Slice DE. Extensions of the Procrustes method for the optimal superimposition of landmarks. Syst Zool. 1990;39:40-59.
30. Bookstein FL. Morphometrics tools for landmark data: Geometry and biology. Cambridge: Cambridge University Press; 1991.p.435.
31. Rohlf J. tpsRelw, version 1.39. Stony Brook, NY: Department of Ecology and Evolution, State University of New York at Stony Brook; 2004. Consultado: marzo de 2008. Disponible en: http://life.bio.sunysb.edu/morph.
32. SAS Institute Inc. JMP® Statistisc and Graphics Guide, version 3.1. Cary, NC: SAS Institute Inc; 1999.p.592.
33. Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33:159-74.
34. Rohlf, J. tpsRegr, version 1.28. Stony Brook, NY: Department of Ecology and Evolution, State University of New York at Stony Brook; 2003. Consultado: marzo de 2008. Disponible en: http://life.bio.sunysb.edu/morph.
35. Sallum M, Schultz T, Foster P, Arostein K, Wirtz R, Wilkerson C. Phylogeny of Anophelinae (Diptera: Culicidae) based of nuclear, ribosomal and mitochondrial DNA secuences. Syst Entomol. 2002; 27:361-82.
36. dos Santos JM, Maia J de F, Tadei WP, Rodríguez GA. Isoenzymatic Variability among five Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera of the Amazon region, Brazil. Mem Inst Oswaldo Cruz. 2003;98:247-53.
37. Sallum MA, Bergo ES, Flores DC, Forattini OP. Systematic Studies on Anopheles galvaoi Causey, Deane & Deane from the Subgenus Nysssorhynchus Blanchard (Diptera: Culicidae). Mem Inst Oswaldo Cruz. 2000;97:1177-89.
38. Gonzales R, Carrejo N. Introducción al estudio taxonómico de Anopheles de Colombia Claves y notas de distribución. Cali: Editorial Litocencoa; 2007. p. 237.
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