Population structure and genetic variability of Rhodnius prolixus (Hemiptera: Reduviidae) from different geographic areas of Colombia.
Keywords:
Chagas disease, epidemiologic surveillance, vector control, Rhodnius, DNA, ribosomal spacer, polymerase chain reaction, polymorphism, restriction fragment length, random
Abstract
Introduction. Rhodnius prolixus is the most important vector of Chagas disease in Colombia. Genetic characterization of this species is useful to understand its potential of dispersion. The distribution of the vector and the genetic population structure are important factors for the adequate implementation of control programs and epidemiological surveillance of Chagas disease.Objective. Genetic relationships were established for populations of R. prolixus collected from several habitat types and representative geographic areas of Colombia. A second aim was to assess its population genetic structure and dispersion across Colombia.
Materials and methods. Genetic comparisons were made from three domestic populations of R. prolixus from (1) Tolima Province, (2) Cundinamarca Province and (3) the Sierra Nevada of Santa Marta in northern Colombia, and (4) one sylvatic population from Casanare. Two molecular techniques were used to evaluate the genetic structure of these populations-analysis of the ITS-2 of ribosomal DNA by PCR/RFLP and RAPDs.
Results. Rhodnius prolixus shows a moderate genetic variability (Fst 0.06-0.15). Among domestic populations, the migration rates found were adequate (Nm>1) to maintain gene flow. A moderate to large degree of genetic differentiation was observed between the sylvatic population from Casanare and the domestic populations from the centre of the country (Tolima and Cundinamarca).
Conclusion. The domestic populations of R. prolixus are homogeneous because genetic flow exists between them, and this is favourable to chemical control, while the sylvatic population clusters apart from the domestic populations. Hence the need to study the genetic structure of the sylvatic foci, their possible dispersion routes and the epidemiological risk that they represents.
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References
1. Moncayo A. Chagas disease: current epidemiological trends after the interruption of vectorial and transfusional transmission in the Southern cone countries. Mem Inst Oswaldo Cruz 2003;98:577-91.
2. Guhl F, Restrepo M, Angulo VM, Antunes CM, Campbell-Lendrum D, Davies C. Lessons from a national survey of Chagas disease transmission in Colombia. Trends Parasitol 2005;21:259-62.
3. Gamboa CJ. Dispersión de Rhodnius prolixus en Venezuela. Bol Dir Malariol Saneam Ambient 1962;3:262-72.
4. Garcia AL, Carrasco HJ, Shofield CJ, Stothard JR, Frame IA, Valente SA, et al. Random amplification of polymorphism DNA as a tool for taxonomics studies of triatomine bugs (Hemiptera: Reduviidae). J Med Entomol 1998;35:38-45.
5. Monteiro FA, Escalante AA, Beard CB. Molecular tools and triatomine systematics: a public health perspective. Trends Parasitol 2001;17:344-7.
6. Bargues MD, Marcilla A, Ramsey JM, Dujardin JP, Schofield CJ, Mas-Coma S. Nuclear rDNA-based molecular clock of the evolution of triatominae (Hemiptera: Reduviidae) vectors of Chagas disease. Mem Inst Oswaldo Cruz 2000;95:567-73.
7. Pacheco RS, Almeida CE, Costa J, Klisiowicz DR, Mas-Coma S, Bargues MD. RAPD analyses and rDNA intergenic-spacer sequences discriminate Brazilian populations of Triatoma rubrovaria. Ann Trop Med
Parasitol 2003;97:757-68.
8. Bargues MD, Marcilla A, Dujardin JP, Mas-Coma S. Triatominae vectors of Chagas disease: a molecular perspective based on nuclear ribosomal DNA markers. Trans R Soc Trop Med Hyg 2002;96(Suppl 1):159-64.
9. García BA, Manfredi C, Fichera L, Segura EL. Variation in mitochondrial 12S and 16S ribosomal DNA sequences in natural populations of Triatoma infestans (Hemiptera, Reduviidae). Am J Trop Med Hyg
2003;68:692-4.
10. Marcilla A, Bargues MD, Ramsey MJ, Magallon-Gastelum E, Salazar-Schettino PM, Abad-Franch F, et al. The ITS-2 of the nuclear rDNA as a molecular marker for populations, species, and phylogenetic
relationships in Triatominae (Hemiptera: Reduviidae) vectors of Chagas disease. Mol Phylogenet Evol 2001;18:136-42.
11. Caldeira RL, Carvalho OS, Mendonça CL, Graeff-Teixeira C, Silva MC, Ben R, et al. Molecular differentiation of Angiostrongylus costaricensis, A. Cantonensis, and A. Vasorum by polymerase chain
reaction-restriction fragment length polymorphism. Mem Inst Oswaldo Cruz 2003;98:1039-43.
12. Jaramillo C, Montaña MF, Castro LR, Vallejo GA, Guhl F. Differentiation and genetic analysis of Rhodnius prolixus and Rhodnius colombiensis by rDNA and RAPD amplification. Mem Inst Oswaldo Cruz
2001;96:1043-8.
13. Ramírez CJ, Jaramillo CA, Delgado M, Pinto NA, Aguilera G, Guhl F. Genetic structure of sylvatic, peridomestic and domestic populations of Triatoma dimidiata (Hemiptera: Reduviidae) from an endemic zone of Boyaca, Colombia. Acta Trop 2005;93:23-9.
14. Borges EC, Romanha AJ, Diotaiuti L. Uso do random amplified polymorphic DNA (RAPD) no estudo populacional do Triatoma brasiliensis Neiva, 1911. Cad Saúde Pública 2000;16:97-100.
15. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae) and their significance as vectors of Chagas disease. Bull Am Mus Nat Hist 1979;163:175-499.
16. Welsh J, Pretzrnan C, Postic D, Girons IS, Baranton G, McClelland M. Genomic fingerprinting by arbitrary primer polymerase chain reaction resolves Borrelia burgeri into three distinct phylogenetic groups. Int J Syst Bacteriol 1992; 42:370-7.
17. Kambhampati S, Black WC 4th, Rai KS. Random amplified polymorphic DNA of mosquito species and populations (Diptera: Culicidae): techniques, statistical analysis, and applications. J Med Entomol 1992;29:939-45.
18. Podani J. SYNTAX. Computer programs for multivariate data analysis in ecology and systematics. Budapest: Scienta Publishing; 1993.
19. Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979;76:5269-73.
20. Nei M. Genetic distance between populations. Am Nat 1972;106:283-92.
21. Wright S. Evolution in Mendelian populations. Genetics 1931;16:97-159.
22. Weir BS, Cockerham CC. Estimating F-statistics for the analysis of population's structure. Evolution 1984;38:1358-70.
23. Lynch M, Milligan BG. Analysis of population genetic structure with RAPD markers. Mol Ecol 1994;3:91-9.
24. Dias JC. Rural resource development and its potential to introduce domestic vectors into a new epidemiological situation. Rev Argent Microbiol 1988;20(Suppl. 1):81-5.
25. Harry M, Galindez I, Cariou ML. Isozyme variability and differentiation between Rhodnius prolixus, R. robustus and R. pictipes, vectors of Chagas disease in Venezuela. Med Vet Entomol 1992;6:37-3.
26. Harry M. Isozymic data question the specific status of some blood-sucking bugs of the genus Rhodnius, vectors of Chagas disease. Trans R Soc Trop Med Hyg 1993;87:492.
27. Harry M. Morphometric variability in the Chagas´disease vector Rhodnius prolixus. Jpn J Genet 1994;69:233-50.
28. Monteiro FA, Lazoski C, Noireau F, Sole-Cava AM. Allozyme relationships among ten species of Rhodniini, showing paraphyly of Rhodnius including Psammolestes. Med Vet Entomol 2002;16:83-90.
29. Monteiro FA, Wesson DM, Dotson EM, Schofield CJ, Beard CB. Phylogeny and molecular taxonomy of the Rhodniini derived from mitochondrial and nuclear DNA sequences. Am J Trop Med Hyg 2000;62:460-5.
30. Monteiro FA, Barrett TV, Fitzpatrick S, Cordon-Rosales C, Feliciangeli D, Beard CB. Molecular phylogeography of the Amazonian Chagas disease vectors Rhodnius prolixus and R. robustus. Mol Ecol
2003;12:997-1006.
31. Abad-Franch F, Monteiro FA. Molecular research and the control of Chagas disease vectors. An Acad Bras Cienc 2005;77:437-54
32. Feliciangeli MD, Campbell-Lendrum D, Martinez C, Gonzales D, Coleman P, Davies C. Chagas disease control in Venezuela: lessons for the Andean region and beyond. Trends Parasitol 2003;19:44-9.
33. Zeledón R. Some historical facts and recent issues related to the presence of Rhodnius prolixus (Stal, 1859) (Hemiptera:Reduviidae) in Central America. Entomología y vectores 2004;11:233-46.
2. Guhl F, Restrepo M, Angulo VM, Antunes CM, Campbell-Lendrum D, Davies C. Lessons from a national survey of Chagas disease transmission in Colombia. Trends Parasitol 2005;21:259-62.
3. Gamboa CJ. Dispersión de Rhodnius prolixus en Venezuela. Bol Dir Malariol Saneam Ambient 1962;3:262-72.
4. Garcia AL, Carrasco HJ, Shofield CJ, Stothard JR, Frame IA, Valente SA, et al. Random amplification of polymorphism DNA as a tool for taxonomics studies of triatomine bugs (Hemiptera: Reduviidae). J Med Entomol 1998;35:38-45.
5. Monteiro FA, Escalante AA, Beard CB. Molecular tools and triatomine systematics: a public health perspective. Trends Parasitol 2001;17:344-7.
6. Bargues MD, Marcilla A, Ramsey JM, Dujardin JP, Schofield CJ, Mas-Coma S. Nuclear rDNA-based molecular clock of the evolution of triatominae (Hemiptera: Reduviidae) vectors of Chagas disease. Mem Inst Oswaldo Cruz 2000;95:567-73.
7. Pacheco RS, Almeida CE, Costa J, Klisiowicz DR, Mas-Coma S, Bargues MD. RAPD analyses and rDNA intergenic-spacer sequences discriminate Brazilian populations of Triatoma rubrovaria. Ann Trop Med
Parasitol 2003;97:757-68.
8. Bargues MD, Marcilla A, Dujardin JP, Mas-Coma S. Triatominae vectors of Chagas disease: a molecular perspective based on nuclear ribosomal DNA markers. Trans R Soc Trop Med Hyg 2002;96(Suppl 1):159-64.
9. García BA, Manfredi C, Fichera L, Segura EL. Variation in mitochondrial 12S and 16S ribosomal DNA sequences in natural populations of Triatoma infestans (Hemiptera, Reduviidae). Am J Trop Med Hyg
2003;68:692-4.
10. Marcilla A, Bargues MD, Ramsey MJ, Magallon-Gastelum E, Salazar-Schettino PM, Abad-Franch F, et al. The ITS-2 of the nuclear rDNA as a molecular marker for populations, species, and phylogenetic
relationships in Triatominae (Hemiptera: Reduviidae) vectors of Chagas disease. Mol Phylogenet Evol 2001;18:136-42.
11. Caldeira RL, Carvalho OS, Mendonça CL, Graeff-Teixeira C, Silva MC, Ben R, et al. Molecular differentiation of Angiostrongylus costaricensis, A. Cantonensis, and A. Vasorum by polymerase chain
reaction-restriction fragment length polymorphism. Mem Inst Oswaldo Cruz 2003;98:1039-43.
12. Jaramillo C, Montaña MF, Castro LR, Vallejo GA, Guhl F. Differentiation and genetic analysis of Rhodnius prolixus and Rhodnius colombiensis by rDNA and RAPD amplification. Mem Inst Oswaldo Cruz
2001;96:1043-8.
13. Ramírez CJ, Jaramillo CA, Delgado M, Pinto NA, Aguilera G, Guhl F. Genetic structure of sylvatic, peridomestic and domestic populations of Triatoma dimidiata (Hemiptera: Reduviidae) from an endemic zone of Boyaca, Colombia. Acta Trop 2005;93:23-9.
14. Borges EC, Romanha AJ, Diotaiuti L. Uso do random amplified polymorphic DNA (RAPD) no estudo populacional do Triatoma brasiliensis Neiva, 1911. Cad Saúde Pública 2000;16:97-100.
15. Lent H, Wygodzinsky P. Revision of the Triatominae (Hemiptera, Reduviidae) and their significance as vectors of Chagas disease. Bull Am Mus Nat Hist 1979;163:175-499.
16. Welsh J, Pretzrnan C, Postic D, Girons IS, Baranton G, McClelland M. Genomic fingerprinting by arbitrary primer polymerase chain reaction resolves Borrelia burgeri into three distinct phylogenetic groups. Int J Syst Bacteriol 1992; 42:370-7.
17. Kambhampati S, Black WC 4th, Rai KS. Random amplified polymorphic DNA of mosquito species and populations (Diptera: Culicidae): techniques, statistical analysis, and applications. J Med Entomol 1992;29:939-45.
18. Podani J. SYNTAX. Computer programs for multivariate data analysis in ecology and systematics. Budapest: Scienta Publishing; 1993.
19. Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979;76:5269-73.
20. Nei M. Genetic distance between populations. Am Nat 1972;106:283-92.
21. Wright S. Evolution in Mendelian populations. Genetics 1931;16:97-159.
22. Weir BS, Cockerham CC. Estimating F-statistics for the analysis of population's structure. Evolution 1984;38:1358-70.
23. Lynch M, Milligan BG. Analysis of population genetic structure with RAPD markers. Mol Ecol 1994;3:91-9.
24. Dias JC. Rural resource development and its potential to introduce domestic vectors into a new epidemiological situation. Rev Argent Microbiol 1988;20(Suppl. 1):81-5.
25. Harry M, Galindez I, Cariou ML. Isozyme variability and differentiation between Rhodnius prolixus, R. robustus and R. pictipes, vectors of Chagas disease in Venezuela. Med Vet Entomol 1992;6:37-3.
26. Harry M. Isozymic data question the specific status of some blood-sucking bugs of the genus Rhodnius, vectors of Chagas disease. Trans R Soc Trop Med Hyg 1993;87:492.
27. Harry M. Morphometric variability in the Chagas´disease vector Rhodnius prolixus. Jpn J Genet 1994;69:233-50.
28. Monteiro FA, Lazoski C, Noireau F, Sole-Cava AM. Allozyme relationships among ten species of Rhodniini, showing paraphyly of Rhodnius including Psammolestes. Med Vet Entomol 2002;16:83-90.
29. Monteiro FA, Wesson DM, Dotson EM, Schofield CJ, Beard CB. Phylogeny and molecular taxonomy of the Rhodniini derived from mitochondrial and nuclear DNA sequences. Am J Trop Med Hyg 2000;62:460-5.
30. Monteiro FA, Barrett TV, Fitzpatrick S, Cordon-Rosales C, Feliciangeli D, Beard CB. Molecular phylogeography of the Amazonian Chagas disease vectors Rhodnius prolixus and R. robustus. Mol Ecol
2003;12:997-1006.
31. Abad-Franch F, Monteiro FA. Molecular research and the control of Chagas disease vectors. An Acad Bras Cienc 2005;77:437-54
32. Feliciangeli MD, Campbell-Lendrum D, Martinez C, Gonzales D, Coleman P, Davies C. Chagas disease control in Venezuela: lessons for the Andean region and beyond. Trends Parasitol 2003;19:44-9.
33. Zeledón R. Some historical facts and recent issues related to the presence of Rhodnius prolixus (Stal, 1859) (Hemiptera:Reduviidae) in Central America. Entomología y vectores 2004;11:233-46.
How to Cite
1.
López DC, Jaramillo C, Guhl F. Population structure and genetic variability of Rhodnius prolixus (Hemiptera: Reduviidae) from different geographic areas of Colombia. biomedica [Internet]. 2007 Jan. 1 [cited 2024 May 17];27(1esp):28-39. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/246
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