Prevalence of enterovirus infection in infants in Armenia, Colombia, 2009
Abstract
Introduction. Despite worldwide circulation of enteroviruses, little information has accumulated on the circulation of enteroviruses in Colombia.
Objective. The prevalence of enterovirus circulation was examined in children under 1 year to identify the most common enterovirus serotypes.
Materials and methods. Fecal samples were collected from 320 children under 1 year of age who attended a first-level health center in the city of Armenia, Colombia, in 2009. Enterovirus detection
was performed by reverse transcription reaction and nested polymerase chain reaction (RT-N-PCR) using generic enterovirus primers. Samples testing positive in the RT-N-PCR were inoculated into cell
cultures susceptible to enterovirus. All isolates were typed by seroneutralization with Lim-Benyesh-Melnick antiserum pools.
Results. Overall, enteroviral RNA was detected in 43 of 320 (13.3%; 95% CI: 9.7 to 17.1) fecal samples by RT-N-PCR. Viral isolation was possible in 26 of 43 (60.4%) of the positive samples. Of these, 15 were Coxsackievirus B (eight CVB1, two CVB2, five CVB5) and 11 Echovirus (six E6 and five E30).
Conclusions. The enteroviral circulation in a population on newly bornes up to 1 year old was 13.3%; the most frequent enterovirus was the same as those serotypes most commonly isolated in other arts
of the world. The use of RT-N-PCR was demonstrably feasible as a tool to monitor the presence of enterovirus in stool samples.
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