Characterization of clinical isolates of Mycobacterium tuberculosis from indigenous peoples of Colombia
Abstract
Introduction: Tuberculosis continues to be a public health priority. Indigenous peoples are vulnerable groups with cultural determinants that increase the risk of the disease.
Objective: To determine molecular epidemiology and phenotypical features and of Mycobacterium tuberculosis isolates from indigenous people in Colombia during the period from 2009 to 2014.
Materials and methods: We conducted an analytical observational study; we analyzed 234 isolates to determine their patterns of sensitivity to antituberculosis drugs and their molecular structures by spoligotyping.
Results: The isolates came from 41 indigenous groups, predominantly the Wayúu (13.10%) and Emberá Chamí (11.35%). We found 102 spoligotypes distributed among seven genetic families (37.2% LAM, 15.8% Haarlem, 8.1% T, 3.4% U, 2.6% S, 2.1% X, and 0.9%, Beijing).
The association analysis showed that the non-clustered isolates were related to prior treatment, relapse, orphan spoligotypes, and the Beijing family. The H family presented an association with the Arhuaco and Camëntŝá indigenous groups, the U family was associated with the Wounaan group, and the T family was associated with the Motilón Barí group.
Conclusions: This is the first national study on M. tuberculosis characterization in indigenous groups. The study evidenced that diagnosis in indigenous people is late. We described 53% of orphan patterns that could be typical of the Colombian indigenous population. The high percentage of grouping by spoligotyping (62%) could indicate cases of active transmission, a situation that should be corroborated using a second genotyping marker. A new Beijing spoligotype (Beijing-like SIT 406) was identified in Colombia.
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References
Organización de Naciones Unidas. Objetivos de Desarrollo Sostenible. Accessed on: February 25, 2017. Available from: http://www.un.org/sustainabledevelopment/es/objetivosde-desarrollo-sosten
World Health Organization. Global Tuberculosis Report, 2017. Accessed on: December 3, 2017. Available from: https://reliefweb.int/sites/reliefweb.int/files/resources/9789241565516-eng.pdf
Instituto Nacional de Salud. Informe de Evento Tuberculosis, Colombia -2016. Accessed on: September 5, 2017. Available from: http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/Informe%20de%20Evento%20Epidemiolgico/TUBERCULOSIS%20%202016.pdf
World Health Organization. Proyecto de estrategia mundial y metas para la prevención la atención y el control de la tuberculosis después de 2015, 67ª Asamblea Mundial de la Salud. Accessed from: March 5, 2017. Available on: http://apps.who.int/gb/ebwha/pdf_files/WHA67-REC1/A67_2014_REC1sp.pdf
Stephens C, Porter J, Nettleton C, Willis R. Disappearing, displaced, and undervalued: A call to action for Indigenous health worldwide. Lancet. 2006;367:2019-28. https://doi.org/10.1016/S0140-6736(06)68892-2
Cardona JA, Hernández AM. Conocimientos, actitudes y prácticas sobre tuberculosis en indígenas zenúes y habitantes de una zona rural colombiana. Rev Cienc Salud. 2013;11:157-71.
Barros D, Costa R, de Albuquerque B, Sadahiro M, Ueleres J. The socioeconomic factors and the indigenous component of tuberculosis in Amazonas. PLoS One. 2016;6:1-10. https://doi.org/10.1371/journal.pone.0158574
Ministerio de Cultura de Colombia. Caracterización y situación actual de los pueblos indígenas. Accessed on: February 10, 2017. Available from: https://www.unicef.org/colombia/pdf/pueblos-indigenas.pdf
Departamento Nacional de Estadística. Colombia una Nación Multicultural: Su diversidad étnica - 2007. Accessed on: March 5, 2017. Available from: http://www.dane.gov.co/files/censo2005/etnia/sys/colombia_nacion.pdf
Medel B, Castellanos M, García M, Martínez R, Decroo T, Zachariah R. Tuberculosis among indigenous municipalities in Mexico: Analysis of case notification and treatment outcomes between 2009 and 2013. Rev Panam Salud Pública. 2016;39:32-7.
Cermeño J, Súnico L, Cermeño V, Julmery J, Godoy G, Hernández de Cuesta I, et al. Tuberculosis y micosis sistémicas en indígenas del Municipio Cedeño, Estado Bolívar, Venezuela. Med Interna Caracas. 2013;29:62-7.
Culqui D, Zabaleta C, Romero J, Bonilla C, Trujillo O, Cueva N. Tuberculosis en poblaciones indígenas del Perú: los aimaras del Perú, 2000-2005. Rev Peru Epidemiol. 2009;13:1-6.
Malacarne J, Giraldo D, Furtado C, Ueleres J, Bastos L, Basta P. Prevalence and factors associated with latent tuberculosis infection in an indigenous population in the Brazilian Amazon. Rev Soc Bras Med Trop. 2016;49:456-64. https://doi.org/10.1590/0037-8682-0220-2016
Tollefson D, Bloss E, Fanning A, Redd JT, Barker K, McCray E. Burden of tuberculosis in indigenous peoples globally: A systematic review. Int J Tuber Lung Dis. 2013;17:1139-50. https://doi.org/10.5588/ijtld.12.0385
Cunha EA, Ferrazoli L, Riley LW, Basta PC, Honer MR, Maia R, et al. Incidence and transmission patterns of tuberculosis among indigenous populations in Brazil. Mem Inst Oswaldo Cruz. 2014;109:108-13. https://doi.org//10.1590/0074-0276130082
Ministerio de la Protección Social - Instituto Nacional de Salud, Agencia Canadiense para el Desarrollo Internacional, Organización Panamericana de la Salud, Organización Mundial de la Salud. Tuberculosis en los pueblos indígenas de Colombia: el reto de la prevención y el control. Accessed on: March 5, 2018. Bogotá: Organización Panamericana de la Salud; 2007. p. 86.
Hernández J, Dávila V, Martínez L, Restrepo L, Grajales N, Toro A, et al. Tuberculosis in indigenous communities of Antioquia, Colombia: Epidemiology and beliefs. J Immigrant Minority Health. 2013;15:10-6. https://doi.org/10.1007/s10903-012-9688-1
García de Viedmaa D, Mokrousovc I, Rastogi N. Innovations in the molecular epidemiology of tuberculosis. Enferm Infecc Microbiol Clin. 2011;29(Supl.1):8-13. https://doi.org/10.1016/S0213-005X(11)70012-X
van Soolingen D, de Haas PE, Kremer K. restriction fragment length polymorphism typing of mycobacteria. In: Parish T, Stoker NG, editors. Mycobacterium tuberculosis protocols. Methods in molecular medicine. New Jersey: Humana Press; 2001. p.165-203.
Kamerbeek J, Schoulds L, Kolk A, Van-agterveld M, Van-Sooligen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35:907-14.
Brudey K, Driscoll J, Rigouts L, Prodinger W, Gori A, Al-Hajoj S, et al. Mycobacterium tuberculosis complex genetic diversity: Mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol. 2006;61-17. https://doi.org/10.1186/1471-2180-6-23
Castro C, Ricardo A, Zabaleta A, Llerena C, Puerto G. Characterization of clinical isolates of Mycobacterium tuberculosis from HIV positive individuals in Colombia, 2012. Biomédica. 2017;37:86-95. https://doi.org/10.7705/biomedica.v37i1.3112
Realpe T, Correa N, Rozo J, Ferro B, Gómez V, Zapata E, et al. Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia. Plos One. 2014;109:1-12. https://doi.org/10.1371/journal.pone.0093848
Puerto G, Erazo L, Wintaco M, Castro C, Ribón W, Guerrero M. Mycobacterium tuberculosis enotypes determined by spoligotyping to be circulating in Colombia between 1999 and 2012 and their possible associations with transmission and susceptibility to first-line drugs. Plos One. 2015;10:1-14. https://doi.org/10.1371/journal.pone.0124308
Cerezo I, Jiménez Y, Hernández J, Zozio T, Murcia M, Rastogi N. A first insight on the population structure of Mycobacterium tuberculosis complex as studied by spoligotyping and MIRU-VNTRs in Bogotá, Colombia. Infect Genet Evol. 2012;12:657-63. https://doi.org/10.1016/j.meegid.2011.07.006
Nieto L, Ferro B, Villegas S, Mehaffy C, Forero L, Moreira C, et al. Characterization of extensively drug-resistant tuberculosis cases from Valle del Cauca, Colombia. J Clin Microbiol. 2012;50:4185-7. https://doi.org//10.1128/JCM.01946-12
Ferro B, Nieto L, Rozo J, Forero L, van Soolingen D. Multidrug-resistant Mycobacterium tuberculosis, Southwestern Colombia. Emerg Infect Dis. 2011;17:1259-62. https://doi.org/10.3201/eid1707.101797
Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems: An application of Simpson’s index of diversity. J Clin Microbiol. 1998;26:2465-6.
Ministerio de Salud y Protección Social. Circular 058 de 2009: Lineamientos para el manejo programático de tuberculosis y lepra. Accessed on: March 5, 2018. Available from: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/DE/DIJ/circular-externa-0007-de-2015.pdf
Ministerio de Salud y Protección Social. Plan Estratégico Colombia Libre de Tuberculosis 2010-2015: Para la Expansión y Fortalecimiento de la Estrategia Alto a la TB. Bogotá D.C: 2009. p. 64. Accessed on: April 5, 2018. Available from: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/plan-monitoreo-evaluacion-tuberculosis.pdf
Li Y, Ehiri J, Tang S, Li D, Bian Y, Lin H, et al. Factors associated with patient, and diagnostic delays in Chinese TB patients: A systematic review and meta-analysis. BMC Med. 2013;11:156. https://doi.org/10.1186/1741-7015-11-156
Bisbicús G, Paí J, Paí, R. Comunicación con los espíritus de la naturaleza para la cacería, pesca, protección, siembra y cosecha en el pueblo indígena Awá de Nariño. Bogotá D.C: Programa Somos Defensores; 2010. p. 90. Accessed on: March 21, 2018. Available from: http://www.onic.org.co/documentos/1467-comunicacion-con-los-espiritus-de-la-naturalezapara-la-caceria-pesca-proteccion-siembra-y-cosecha-en-el-pueblo-indigena-awa-de-narino
Programa de las Naciones Unidas para el Desarrollo. Pueblos indígenas. Diálogo entre culturas. Cuaderno del Informe de Desarrollo Humano de Colombia. Bogotá: PNUD; 2012. p. 96. Accessed on: March 30, 2018. Available from: http://www.co.undp.org/content/colombia/es/home/library/human_development/pueblos-indi_genas---dialogo-entre-culturas.html
Sieder R, Sierra M. Indigenous women’s access to justice in Latin America. CMI Working Paper WP; 2010. Accessed on: September 22, 2017. Available from: https://www.cmi.no/publications/3880-indigenous-womens-access-to-justice-in-latin
Oshi D, Oshi S, Alobu I, Ukwaja K. Gender-related factors influencing women’s health seeking for tuberculosis care in Ebonyi State, Nigeria. J Biosoc Sci. 2016;48:37-50. https://doi.org/10.1017/S0021932014000534
UNICEF. Los pueblos indígenas en Colombia: derechos, políticas y desafíos. Bogotá D.C: UNICEF; 2009. p. 91. Accessed on: September 5, 2017. Available from: https://www.unicef.org/colombia/pdf/pueblos-indigenas.pdf
Ribeiro M, Dalla l, Silva S. Factores asociados el diagnóstico precoz y tardío de la tuberculosis: estudio descriptivo. Online Braz J Nurs. 2014;13:62-71.
Gaviria M, Henao H, Martínez T, Bernal E. Papel del personal de salud en el diagnóstico tardío de la tuberculosis pulmonar en adultos de Medellín, Colombia. Rev Panam Salud Pública. 2010;27:83-92.
Dorronsoro I, Torroba l. Microbiología de la tuberculosis. Anales Sis San Navarra. 2007;30(Supl. 2):67-85.
Chen Y, Chang J, Huang W, Kuo S, Yeh J, Lee J, et al. Molecular epidemiology of Mycobacterium tuberculosis in aboriginal peoples of Taiwan, 2006 -2011. J Infect. 2014;68:332-7. https://doi.org/10.1016/j.jinf.2013.12.004
Álvarez N, Haft D, Hurtado UA, Robledo J, Rouzaud F. Whole-genome sequence of a Beijing extensively drug-resistant Mycobacterium tuberculosis clinical isolate from Buenaventura, Colombia. Genome Announc. 2016;4:e01549-15. https://doi.org//10.1128/genomeA.01549-15
Murcia MI, Manotas M, Jiménez Y, Hernández J, Cerezo I, Cortés MI, et al. First case of multidrug-resistant tuberculosis caused by a rare ‘‘Beijing-like’’. Infect Genet Evol. 2010;10:678-81. https://doi.org/10.1016/j.meegid.2010.03.010
Flores S, Morfín R, Rodríguez E, González E, Pérez H, Bocanegra V, et al. Genetic diversity of Mycobacterium tuberculosis from Guadalajara, Mexico and identification of a rare multidrug resistant Beijing genotype. PLoS One. 2015;10:1-11. https://doi.org/10.1371/journal.pone.0118095
Idrovo A. Raíces históricas, sociales y epidemiológicas de la tuberculosis en Bogotá, Colombia. Biomédica. 2004;24356-65. https://doi.org/10.7705/biomedica.v24i4.1285
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