Seroprevalence of Brucella canis in canines from a dog shelter in Bogotá, Colombia

Astrid-Jullieth Laverde, Daniela Restrepo-Botero , Diego Hernández-Pulido , José Luis Rodríguez-Bautista , Isabel-Sofía Sandoval, .

Keywords: Brucellosis, dogs, zoonoses, chromatography, affinity, public health

Abstract

Introduction: The risk of Brucella canis infection in humans and dogs has increased due to the permanent exposure to asymptomatic carrier dogs. In Colombia, there is evidence of B. canis infection in humans living with dogs. In the case of Bogotá, an additional concern is the lack of updated information related to the prevalence of the infection in dogs.
Objective: To determine the seroprevalence of infection by B. canis in dogs intended for adoption programs in Bogotá.
Materials and methods: By means of a descriptive cross-sectional study carried out in a dog shelter in Bogotá, anti-B. canis IgG antibodies were detected in the serum from 51 dogs 28 females and 23 males) using a lateral-flow immunochromatographic test. Additionally, seropositive animals were analyzed with PCR to detect Brucella spp DNA.
Results: Brucella canis seroprevalence was 1.96% (1/51). The seropositive dog was an asymptomatic three-year-old she-dog in which no bacteria DNA was detected in the blood through PCR.
Conclusions: The seroprevalence determined in this study represented by a single dog with anti-B. canis IgG can be considered a potential risk both for canine and human populations since this single dog could have a persistent infection capable of spreading the bacteria.

Downloads

Download data is not yet available.

References

Batinga MC, Dos Santos JC, Lima JT, Bigotto MF, Muner K, Faita T, et al. Comparison of three methods for recovery of Brucella canis DNA from canine blood samples. J Microbiol Methods. 2017;143:26-31. https://doi.org/10.1016/j.mimet.2017.08.019

Hensel ME, Negron M, Arenas-Gamboa AM. Brucellosis in dogs and public health risk. Emerg Infect Dis. 2018;24:1401-6. https://doi.org/10.3201/eid2408.171171

Pujol M, Borie C, Montoya M, Ferreira A, Vernal R. Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation. Comp Immunol Microbiol Infect Dis. 2019;62:68-75. https://doi.org/10.1016/j.cimid.2018.11.017

Olsen SC, Palmer MV. Advancement of knowledge of Brucella over the past 50 years. Vet Pathol. 2014;51:1076-89. https://doi.org/10.1177/0300985814540545

Chacón-Díaz C, Altamirano-Silva P, González-Espinoza G, Medina MC, Alfaro-Alarcón A, Bouza-Mora L, et al. Brucella canis is an intracellular pathogen that induces a lower proinflammatory response than smooth zoonotic counterparts. Infect Immun. 2015;83:4861-70. https://doi.org/10.1128/IAI.00995-15

Gyuranecz M, Szeredi L, Rónai Z, Dénes B, Dencso L, Dán Á, et al. Detection of Brucella canis-induced reproductive diseases in a Kennel. J Vet Diagnostic Investig. 2011;23:143-7. https://doi.org/10.1177/104063871102300127

Byndloss MX, Tsolis RM. Brucella spp. virulence factors and immunity. Annu Rev Anim Biosci. 2016;4:111-27. https://doi.org/10.1146/annurev-animal-021815-111326

Forbes JN, Frederick SW, Savage MY, Cross AR. Brucella canis sacroiliitis and discospondylitis in a dog. Can Vet J. 2019;60:1301-4.

Ledbetter EC, Landry MP, Stokol T, Kern TJ, Messick JB. Brucella canis endophthalmitis in 3 dogs: Clinical features, diagnosis, and treatment. Vet Ophthalmol. 2009;12:183-91. https://doi.org/10.1111/j.1463-5224.2009.00690.x

Keid LB, Chiebao DP, Batinga MC, Faita T, Diniz JA, Oliveira TM, et al. Brucella canis infection in dogs from commercial breeding kennels in Brazil. Transbound Emerg Dis. 2017;64:691-7. https://doi.org/10.1111/tbed.12632

Johnson CA, Carter TD, Dunn JR, Baer SR, Schalow MM, Bellay YM, et al. Investigation and characterization of Brucella canis infections in pet-quality dogs and associated human exposures during a 2007–2016 outbreak in Michigan. J Am Vet Med Assoc. 2018;253:322-36. https://doi.org/10.2460/javma.253.3.322

Carmichael LE, Joubert JC. Transmission of Brucella canis by contact exposure. Cornell Vet. 1988;78:63-73.

Kauffman LK, Petersen CA. Canine brucellosis: Old foe and reemerging scourge. Vet Clin North Am Small Anim Pract. 2019;49:763-79. https://doi.org/10.1016/j.cvsm.2019.02.013

De Souza TD, De Carvalho TF, Mol JP, Lopes JV, Silva MF, Da Paixão TA, et al. Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates. Sci Rep. 2018;8:7203-12. https://doi.org/10.1038/s41598-018-25651-x

Wanke MM, Delpino MV, Baldi PC. Use of enrofloxacin in the treatment of canine brucellosis in a dog kennel (clinical trial). Theriogenology. 2006;66:1573-8. https://doi.org/10.1016/j.theriogenology

Boeri EJ, Escobar GI, Ayala SM, Hasan D, Lucero NE. Tracking dogs infected with Brucella canis after antibiotic treatment. Arch Anim Husb Dairy Sci. 2018;1:1-4. https://doi.org/10.33552/AAHDS.2018.01.000505

Lucero NE, Corazza R, Almuzara MN, Reynes E, Escobar GI, Boeri E, et al. Human Brucella canis outbreak linked to infection in dogs. Epidemiol Infect. 2010;138:280-5. https://doi.org/10.1017/S0950268809990525

Krueger WS, Lucero NE, Brower A, Heil GL, Gray GC. Evidence for unapparent Brucella canis infections among adults with occupational exposure to dogs. Zoonoses Public Health. 2014;61:509-18. https://doi.org/10.1111/zph.12102

Castrillón-Salazar L, Giraldo-Echeverri CA, Sánchez-Jiménez MM, Olivera-Angel M. Factores asociados con la seropositividad a Brucella canis en criaderos caninos de dos regiones de Antioquia, Colombia. Cad Saúde Pública. 2013;29:1955-73. https://doi.org/10.1590/0102-311X00133013

Makloski CL. Canine brucellosis management. Vet Clin North Am Small Anim Pract. 2011;41:1209-19. https://doi.org/10.1016/j.cvsm.2011.08.001

Olivera M, Di-Lorenzo C. Aislamiento de Brucella canis en un humano conviviente con caninos infectados. Informe de un caso. Colomb Med. 2009;40:218-20.

Sánchez-Jiménez MM, Giraldo-Echeverri CA, Olivera-Angel M. Infección por Brucella canis en humanos: propuesta de un modelo teórico de infección a través de la ruta oral. Infectio. 2013;17:193-200. https://doi.org/10.1016/S0123-9392(13)70731-8

Giraldo-Echeverri CA, Ruiz-Cortés ZT, Olivera-Ángel M. Brucella canis en Medellín (Colombia), un problema actual. Revista UDCA Actualidad & Divulgación Científica. 2009;12:51-5.

Whitten TV, Brayshaw G, Patnayak D, Álvarez J, Larson CM, Root Kustritz M, et al. Seroprevalence of Brucella canis antibodies in dogs entering a Minnesota humane society, Minnesota, 2016-2017. Prev Vet Med. 2019;168:90-4. https://doi.org/10.1016/j.prevetmed.2019.04.015

Agudelo P, Molina V, Arias V, Madrigal E. Estudio serológico de brucelosis canina en dos albergues del municipio de Envigado, Colombia (2011). Revista de la Facultad de Medicina Veterinaria y de Zootecnia. 2014;61:134-41. https://doi.org/10.15446/rfmvz.v61n2.44676

Uribe R, Delgado K. Determinación de la presencia de Brucella canis en caninos de dos refugios de la ciudad de Bucaramanga en 2012. CES Medicina Veterinaria y Zootecnia. 2013;8:95-103.

Ruiz-Buitrago JD, Giraldo-Echeverri CA, López LV, Chica JF. Seroprevalencia de Brucella canis en perros callejeros del Centro de Bienestar Animal “La Perla”, Medellín (Colombia), 2008. Revista Colombiana de Ciencias Pecuarias. 2010;23:166-72.

Tuemmers C, Lüders C, Rojas C, Serri M, Castillo C, Espinoza R. Detección de Brucella canis por método de inmunocromatografía en perros vagos capturados en la ciudad de Temuco, Chile, 2011. Rev Chil Infectol. 2013;30:395-401. https://doi.org/10.4067/S0716-10182013000400007

Hubbard K, Wang M, Smith DR. Seroprevalence of brucellosis in Mississippi shelter dogs. Prev Vet Med. 2018;159:82-6. https://doi.org/10.1016/j.prevetmed.2018.09.002

Chinyoka S, Dhliwayo S, Marabini L, Dutlow K, Matope G, Pfukenyi DM. Serological survey of Brucella canis in dogs in urban Harare and selected rural communities in Zimbabwe. J S Afr Vet Assoc. 2014;85:e1-e5. https://doi.org/10.4102/jsava.v85i1.1087

Hafemann DCM, Merlini LS, Gonçalves DD, Fortes MS, Navarro IT, Chiderolli RT, et al. Detection of anti-Leptospira spp., anti-Brucella spp., and anti-Toxoplasma gondii antibodies in stray dogs. J Venom Anim Toxins Incl Trop Dis. 2013;19:23-7. https://doi.org/10.1186/1678-9199-19-23

de Paula Dreer M, Gonçalves D, da Silva Caetano I, Gerônimo E, Menegas P, Bergo D, et al. Toxoplasmosis, leptospirosis and brucellosis in stray dogs housed at the shelter in Umuarama municipality, Paraná, Brazil. J Venom Anim Toxins Incl Trop Dis. 2013;19:23-7. https://doi.org/10.1186/1678-9199-19-23

Anigen Rapid C. Brucella Ab Test Kit. Fecha de consulta: 28 de septiembre de 2020. Disponible en: http://www.vetpetspharma.com/images/productos/canino/CBrucellaAb/CBrucellaAb.pdf

Kim D, Park J, Kim SJ, Soh YM, Kim HM, Oh BH, et al. Brucella immunogenic BP26 forms a channel-like structure. J Mol Biol. 2013;425:1119-26. https://doi.org/10.1016/j.jmb.2013.01.015

Sánchez-Jiménez MM, Ortiz-Román LF, Castrillón-Salazar LL, Giraldo-Echeverri CA, Olivera-Angel M. Application of a polymerase chain reaction test for the detection of Brucella canis from clinical samples of canines and humans. Revista Colombiana de Ciencias Pecuarias. 2014;27:3-11.

Olivera M, Giraldo CA, Di Lorenzo C. PCR identification of Brucella canis in canine blood and milk. A case report. Arch Med Vet. 2011;43:295-8. https://doi.org/10.4067/S0301-732X2011000300012

Cárdenas D, Obando J, Moreno C, Mesa L, Ortiz A. Seroprevalencia de Brucella canis en la población canina del centro de zoonosis de la ciudad de Villavicencio. Revista Electrónica de Veterinaria. 2017;18:1-11.

Pardo A, Pérez C, Góngora A, Gómez L, Moreno A. Encuesta exploratoria de infección por Brucella canis en perros de Villavicencio-Colombia. Rev MVZ Córdoba. 2009;14:1690-6. https://doi.org/10.21897/rmvz.352

Agudelo-Flórez P, Castro B, Rojo-Ospina R, Henao-Villegas S. Seroprevalencia y factores de riesgo para brucelosis canina en perros domésticos de once comunas de la ciudad de Medellín-Colombia. Rev Salud Pública (Bogotá). 2012;14:644-56.

Castrillón-Salazar L, López-Diez L, Sánchez-Nodarse R, Sanabria-González W, Henao-Correa E, Olivera-Angel M. Prevalencia de presentación de algunos agentes zoonóticos transmitidos por caninos y felinos en Medellín, Colombia. Rev MVZ Córdoba. 2019;24:7119-26. https://doi.org/10.21897/rmvz.1524

Ballut JC, Calderón A, Rodríguez V. Brucelosis en hembras caninas en Montería (Colombia): un problema para la salud pública. Biosalud. 2013;12:66-74.

Kaltungo BY, Saidu SNA, Sackey AKB, Kazeem HM. A review on diagnostic techniques for brucellosis. Afr J Biotechnol. 2014;13:1-10. https://doi.org/10.5897/AJB2013.13442

Carmichael LE, Shin SJ. Canine brucellosis: A diagnostician’s dilemma. Semin Vet Med Surg Small Anim. 1996;11:161-5. https://doi.org/10.1016/s1096-2867(96)80028-4

Hollett RB. Canine brucellosis: Outbreaks and compliance. Theriogenology. 2006;66:575-87. https://doi.org/10.1016/j.theriogenology.2006.04.011

Greene CE, Carmichael LE. Canine brucellosis. En: Sykes J, Greene J, editors. Infectious Diseases of the Dog and Cat. 4th edition. London: Elsevier Health Sciences; 2012. p. 398-411.

Cosford KL. Brucella canis: An update on research and clinical management. Can Vet J. 2018;59:74-81.

Ardoino SM, Baruta DA, Toso RE. Brucelosis canina. Ciencia Veterinaria. 2006;8:49-60. 48. Wanke M. Canine brucellosis. Anim Reprod Sci. 2004;82-83:195-207. https://doi.org/10.1016/j.anireprosci.2004.05.005

De Souza TD, De Carvalho TF, Mol JPDS, Lopes JVM, Silva MF, Da Paixão TA, et al. Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates. Sci Rep. 2018;8:7203-12. https://doi.org/10.1038/s41598-018-25651-x

Aras Z, Uçan US. Detection of Brucella canis from inguinal lymph nodes of naturally infected dogs by PCR. Theriogenology. 2010;74:658-62. https://doi.org/10.1016/j.theriogenology.2010.03.023

Daly R, Willis KC, Wood J, Brown K, Brown D, Beguin-Strong T, et al. Seroprevalence of Brucella canis in dogs rescued from South Dakota Indian reservations, 2015-2019. Prev Vet Med. 2020;184:105157. https://doi.org/10.1016/j.prevetmed.2020.105157

Oosthuizen J, Oguttu JW, Etsebeth C, Gouws WF, Fasina FO. Risk factors associated with the occurrence of Brucella canis seropositivity in dogs within selected provinces of South Africa. J S Afr Vet Assoc. 2019;90:e1-e8. https://doi.org/10.4102/jsava.v90i0.1956

Lawaczeck E, Toporek J, Cwikla J, Mathison BA. Brucella canis in a HIV-infected patient. Zoonoses Public Health. 2011;58:150-2. https://doi.org/10.1111/j.1863-2378.2010.01334.x

Lucero NE, Maldonado PI, Kaufman S, Escobar GI, Boeri E, Jacob NR. Brucella canis causing infection in an HIV-infected patient. Vector Borne Zoonotic Dis. 2010;10:527-9. https://doi.org/10.1089/vbz.2009.0034

Dentinger CM, Jacob K, Lee LV, Méndez HA, Chotikanatis K, Mcdonough PL, et al. Human Brucella canis infection and subsequent laboratory exposures associated with a puppy, New York City, 2012. Zoonoses Public Health. 2015;62:407-14. https://doi.org/10.1111/zph.12163

Lucero NE, Escobar GI, Ayala SM, Jacob N. Diagnosis of human brucellosis caused by Brucella canis. J Med Microbiol. 2005;54:457-61. https://doi.org/10.1099/jmm.0.45927-0

Tosi MF, Nelson TJ. Brucella canis infection in a 17-month-old child successfully treated with moxalactam. J Pediatr. 1982;101:725-7. https://doi.org/10.1016/s0022-3476(82)80301-6

Nomura A, Imaoka K, Imanishi H, Shimizu H, Nagura F, Maeda K, et al. Human Brucella canis infections diagnosed by blood culture. Emerg Infect Dis. 2010;16:1183-5. https://doi.org/10.3201/eid1607.090209

Swenson RM, Carmichael LE, Cundy KR. Human infection with Brucella canis. Ann Intern Med. 1972;76:435-8. https://doi.org/10.7326/0003-4819-76-3-435

Smith LM, Hartmann S, Munteanu AM, Villa PD, Quinnell RJ, Collins LM. The effectiveness of dog population management: A systematic review. Animals (Basel). 2019;9:1020-49. https://doi.org/10.3390/ani9121020

Maia C, Almeida B, Coimbra M, Fernandes MC, Cristóvão JM, Ramos C, et al. Bacterial and protozoal agents of canine vector-borne diseases in the blood of domestic and stray dogs from southern Portugal. Parasit Vectors. 2015;8:138-44. https://doi.org/10.1186/s13071-015-0759-8

How to Cite
1.
Laverde A-J, Restrepo-Botero D, Hernández-Pulido D, Rodríguez-Bautista JL, Sandoval I-S. Seroprevalence of Brucella canis in canines from a dog shelter in Bogotá, Colombia. biomedica [Internet]. 2021 Jun. 29 [cited 2024 May 17];41(2):260-7. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/5409

Some similar items:

Published
2021-06-29
Section
Original articles

Altmetric

Article metrics
Abstract views
Galley vies
PDF Views
HTML views
Other views
QR Code