Molecular characterization of Colombian invasive serotype 5 Streptococcus pneumoniae isolates recovered between 1994 and 2004.
Keywords:
Streptococcus pneumoniae, meningitis, pneumonia, electrophoresis, pulsed-field gel, clones, tetracycline resistance, chloramphenicol resistance
Abstract
Background. Streptococcus pneumoniae serotype 5 is an important cause of invasive disease in Colombia where the circulation of the clone Colombia5-19, penicillin susceptible but tetracycline and chloramphenicol resistant, has been demonstrated.Objective. To establish the genetic relatedness among Colombian invasive S. pneumoniae serotype 5 isolates, recovered between 1994 and 2004, and the clone Colombia(5)-19.
Materials And Methods. Eighty three isolates with penicillin, vancomycin, ceftriaxone, erythromycin, trimethoprim-sulfametoxazole, chloramphenicol and tetracycline susceptibility patterns were studied. Of these isolates, 29 were recovered from children less than 5 years of age. DNA restriction patterns of all isolates were determined for pulsed field gel electrophoresis, using the Smal enzyme. Genetic similarity among isolates and the clone Colombia(5)-19 was established according to Tenover's criteria and the Fingerprintingä II program.
Results. All isolates were related with the Colombia(5)-19 clone and belonged to the electrophoretic pattern A, of which 17 subtypes were derived. Most of the isolates belonged to patterns A (38,6%), A8 (21,7%) and A5 (12%), the other 23 isolates belonged to 15 electrophoretic patterns. Thirty -four isolates resistant both to tetracycline and chloramphenicol were related with electrophoretic patterns A (n = 32), A16 (n = 1) and A28 (n = 1), which have a band of 340 Kb in common with the clone.
Conclusion. These results showed the continuing circulation of S pneumoniae serotype 5 isolates in the country, and which are genetically related with the clone Colombia(5)-19.
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1.
Firacative C, Moreno J, Castañeda E. Molecular characterization of Colombian invasive serotype 5 Streptococcus pneumoniae isolates recovered between 1994 and 2004. biomedica [Internet]. 2006 Jun. 1 [cited 2024 May 19];26(2):295-301. Available from: https://revistabiomedica.org/index.php/biomedica/article/view/1419
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Published
2006-06-01
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Short communication
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