Laboratory susceptibility tests of Aedes aegypti and Culex quinquefasciatus larvae to the entomopathogenic nematode Heterorhabditis bacteriophora
Abstract
Introduction: Aedes aegypti is the vector of dengue, yellow fever, Zika and Chikungunya viruses, and Culex quinquefasciatus is the vector of St. Louis and West Nile encephalitis viruses.
Objective: To evaluate infectivity of Heterorhabditis bacteriophora N4 in C. quinquefasciatus and A. aegypti larvae under laboratory conditions.
Materials and methods: Thirty second-instar larvae of the two mosquito species were exposed each to different doses (0:1, 1:1, 5:1, 15:1, 100:1, 500:1, 750:1 and 1,500:1) of nematode infective juveniles. Four replications per dose were performed.
Results: Parasitism varied between 2.5 and 80 % in C. quinquefasciatus, and between 4.2 and 92.5 % in A. aegypti, with significant differences between doses (p<0.0001). DL50 were: 160.8 infective juveniles per larva for C. quinquefasciatus and 113.6 infective juveniles per larva for A. aegypti. In C. quinquefasciatus, 4 to 6 % of the infective juveniles developed to adults and in A. aegypti, 12- 61 %. In A. aegypti the emergence of new infective juveniles occurred with 100:1, 500:1, 750:1 and 1,500:1 infective juveniles per larva, and in C. quinquefasciatus, with 1,500:1 infective juveniles per larva. Melanization of infective juveniles was observed in both mosquito species.
Conclusion: The susceptibility of these mosquito species to parasitism of an indigenous isolate of H. bacteriophora in the laboratory was demonstrated. Heterorhabditis bacteriophora N4 could be an efficient biological control agent.
Downloads
References
Almirón WR, Crocco L. Mosquitos urbanos transmisores de dengue y encefalitis de San Luis. Manual de capacitación docente. Córdoba, Argentina: Ed. Universitas; 2007. p. 48.
Santamarina-Mijares A, Pérez-Pacheco R, Martínez SH. Susceptibilidad de las larvas de Aedes aegypti al parasitismo por Romanomermis culicivorax en condiciones de laboratorio y de campo en Oaxaca, México. Rev Panam Salud Pública. 2000;8:299-304. https://doi.org/10.1590/S1020-49892000001000001
Grech, M, Visintín A, Laurito M, Estallo E, Lorenzo P, Roccia I, et al. New records of mosquito species (Diptera: Culicidae) from Neuquén and La Rioja provinces, Argentina. Rev Saúde Pública. 2012;46:387-9. https://doi.org/10.1590/S0034-89102012005000007
Sabattini M. Importancia actual de los arbovirus en Argentina. Sitio Argentino de Producción Animal. 2010;266:1-4.
Ministerio de Salud de la Nación. Fiebre chikunguña - Circulación viral en Bolivia y Paraguay. Fortalecimiento de la vigilancia y acciones de prevención en localidades fronterizas. Fecha de consulta: 11 de julio de 2016. Disponible en: http://www.msal.gob.ar/images/stories/epidemiologia/alertas-2015/10-02-2015-alerta-1chikun.pdf
Ministerio de Salud de la Nación. Comunicación. OPS/OMS alerta por situación de fiebre amarilla. Fecha de consulta: 12 de julio de 2016. Disponible en: http://www.
msal.gob.ar/images/stories/epidemiologia/alertas-2016/05-05-2016-comunicacion-por-fiebre-amarilla.pdf
Ministerio de Salud de la Nación. Comunicación. Transmisión local de virus Zika en la Argentina. Fecha de consulta: 12 de julio de 2016. Disponible en: http://www.msal.gob.ar/images/stories/epidemiologia/alertas-2016/26-02-2016-comunicacion-transmision-local-zika.pdf
Organización Mundial de la Salud. Dengue y dengue grave. Nota descriptiva. Fecha de consulta: 12 de julio de 2016. Disponible en: http://www.who.int/mediacentre/
factsheets/fs117/es/
La Nación. Dengue: alertan que hay un 70 % más de casos que en 2009. Fecha de consulta: 13 de julio de 2016. Disponible en: http://www.lanacion.com.ar/1892515
Sabattini MS, Avilés G, Monath TP. Historical, epidemiological and ecological aspects of arboviruses in Argentina: Flaviviridae, Bunyaviridae and Rhabdoviridae. En: Travassos da Rosa AP, Vasconcelos PF, Travassos da Rosa JF, editores. An overview of arbovirology in Brazil and neighbouring countries. Belem, Parà, Brasil: Instituto Evandro Chagas; 1998. p. 113-34.
Spinsanti L, Basquiera A, Bulacio S, Kim S, Somale V, Ré V, et al. St. Louis encephalitis in Argentina: The first case reported in the last seventeen years. Emerg Infect Dis. 2003;9:271-3. https://doi.org/10.3201/eid0902.020301
Díaz LA, Almirón WR, Ludueña-Almeida F, Spinsanti LI, Contigiani MS. Vigilancia del virus encefalitis de San Luis y mosquitos (Diptera: Culicidae) en la provincia de Córdoba, Argentina. Entomología y Vectores. 2003;10:551-66.
Ministerio de Salud de la Nación. Brote de encefalitis de San Luis en Pergamino. Fecha de consulta: 11 de julio de 2016. Disponible en: http://www.msal.gob.ar/images/stories/epidemiologia/alertas-2015/24-04-2015-alertacasos-encefalitis-san-luis.pdf
Enría DA, Morales MA. Temas de Zoonosis IV. Virus del Oeste del Nilo: su emergencia en las Américas. Fecha de consulta: 12 de julio de 2016. Disponible en: http://repositorio.anlis.gov.ar/xmlui/bitstream/handle/ 123456 789/529/RevistaVeterinariaArgentina,2010,XXX,303..pdf?sequence=1
Solís-Rojas C. Alternativas diversas para el combate y optimización de estrategias en el manejo de mosquitos culícidos en la zona metropolitana de Monterrey N. L.
México (tesis). San Nicolás de Los Garza, Nuevo León: Universidad Autónoma de Nuevo León; 2000.
Adams BJ, Nguyen KB. Taxonomy and systematic. En: Gaugler R, editor. Entomopathogenic Nematology. Wallingford, UK: CAB International; 2002. p. 1-33.
Lewis EE, Campbell J, Griyn CH, Kaya H, Peters A. Behavioral ecology of entomopathogenic nematodes. Biological Control. 2006;38:66-79. https://doi.org/10.1016/j.
biocontrol.2005.11.007
Gaugler R, Kaya HK. Entomopathogenic nematodes in biological control. Boston: CRC Press; 1990. p. 365.
Molta NB, Hominick WM. Dose- and time-response assessments of Heterorhabditis heliothidis and Steinernema feltiae (Nematoda: Rhabditida) against Aedes aegypti larvae. Entomophaga.1989;34:485-93. https://doi.org/10.1007/BF02374386
Peschiutta ML, Cagnolo SR, Almirón WR. Susceptibility of larvae of Aedes aegypti (Linnaeus) (Diptera: Culicidae) to entomopathogenic nematode Heterorhabditis bacteriophora (Poinar) (Rhabditida: Heterorhabditidae). Rev Soc Entomol Argent. 2014;73:99-108.
Poinar GO Jr, Kaul HN. Parasitism of the mosquito Culex pipiens by the nematode Heterorhabditis bacteriophora. J Invertebr Pathol.1982;39:382-7. https://doi.org/10.1016/0022-2011(82)90063-5
de Doucet MM, Giayetto AL. Gama de huéspedes y especificidad en Heterorhabditis bacteriophora Poinar (Heterorhabditidae: Nematoda). Nematología mediterránea.
;22:171-7.
de Doucet MM, Bertolotti MA, Giayetto AL, Miranda MB. Host range, specificity, and virulence of Steinernema feltiae, Steinernema rarum, and Heterorhabditis bacteriophora (Steinernematidae and Heterorhabditidae) from Argentina. J Invertebr Pathol.1999;73:237-42. https://doi.org/10.1006/jipa.1998.4831
Cagnolo SR, Almirón WR. Capacity of the terrestrial entomopathogenic nematode Steinernema rarum (Rhabditida: Steinernematidae) to parasite Culex apicinus larvae (Diptera: Culicidae). Rev Soc Entomol Argent. 2010;69:141-5.
Fairbairn JP, Fenton A, Norman RA, Hudson PJ. The invasion efficiency of the entomopathogenic nematode Steinernema feltiae (Rhabditidae: Steinernematidae). Asp
Appl Biol. 1999;53:83-8.
El Sadawy HA, Saleh MM. Infectivity of Egyptian and imported entomopathogenic nematodes under different temperatures. International Journal of Nematology. 1999;9:
-5.
Kaya HK, Stock SP. Techniques in insect nematology. En: Lacey L, editor. Manual of techniques in insect pathology. Londres, UK: Academic Press; 1997. p. 281-324.
Domínguez, CM, Ludueña-Almeida F, Almirón WR. Dinámica poblacional de Aedes aegypti (Diptera: Culicidae) en Córdoba Capital. Revista de la Sociedad Entomológica
Argentina. 2000;59:41-50.
Di Rienzo JA, Casanoves F, Balzarini MG, González L, Tablada M, Robledo CW. InfoStat versión 2013. Grupo InfoStat. Córdoba: Universidad Nacional de Córdoba; 2013.
Zohdy NM, Shamseldean MM, Abd-El-Samie EM, Hamama HM. Efficacy of the Steinernematid and Heterorhabditid nematodes for controlling the mosquito, Culex quinquefasciatus Say (Diptera: Culicidae). Journal of Mosquito Research. 2013;3:33-44. https://doi.org/10.5376/jmr.2013.03.0005
Dadd RH. Size limitations on the infectivity of mosquito larvae by nematodes during filter-feeding. J InvertebrPathol. 1971;18:246-51. https://doi.org/10.1016/0022-2011(71)90152-2
Bedding RA, Molyneux AS. Penetration of insect cuticle by infective juveniles of Heterorhabditis spp. (Heterorhabditidae: Nematoda). Nematologica. 1982;28:354-9.
Baker GL, Capinera JH. Nematodes and nematomorphs as control agents of grasshoppers and locusts. Memories of the Entomological Society of Canada. 1997;171:157-211.
https://doi.org/10.4039/entm129171157-1 34. Poinar GO Jr. Taxonomy and biology of Steinernematidae and Heterorhabditidae. En: Gaugler R, Kaya HK, editors. Entomopathogenic Nematodes in Biological Control. Boca Raton: CRC Press; 1990. p. 23-61.
Some similar items:
- Clara Beatriz Ocampo, Camila González, Carlos A. Morales, Mauricio Pérez, Dawn Wesson, Charles S. Apperson, Evaluation of community-based strategies for Aedes aegypti control inside houses , Biomedica: Vol. 29 No. 2 (2009)
- Larry Niño, Use of the function semivariogram and kriging estimation in the spacial analysis of Aedes aegypti (Diptera: Culicidae) distributions , Biomedica: Vol. 28 No. 4 (2008)
- Ronald Maestre, Consuelo Vergara Sanchez, Guillermo Berrueco Rodríguez, Betsy Bello Novoa, Helena Brochero, Presence of Haemagogus equinus Theobald, 1903 (Diptera: Culicidae), in Soledad and Malambo, in the Province of Atlántico, Colombia , Biomedica: Vol. 28 No. 1 (2008)
- Claudia M.E. Romero, Humberto Llinás, Andrew K.I. Falconar, Evaluation of a rapid water-surface sweeping method to curately estimate numbers of Aedes aegypti (Diptera: Culicidae) late larval stages in large water-storage containers: comparison with pupal estimates , Biomedica: Vol. 30 No. 2 (2010)
- Susanne Ardila-Roldán, Liliana Santacoloma, Helena Brochero, Status of insecticide susceptibility of public health use in natural populations of Aedes aegypti (Diptera: Culicidae) of Casanare, Colombia , Biomedica: Vol. 33 No. 3 (2013)
- César A. Rodriguez, Diana C. Gallego, José A. González, Paola A. Luna, Mitzilene Navarro, Germán A. Ramírez, Mosquitoe breeding sites and fauna ln Armenia's urban area aiier the January 25, 1999, earthquake , Biomedica: Vol. 21 No. 2 (2001)
- Ariosto Ardila, Jesús Escovar, Felio Bello, Characteristics of new cell cultures derived from embryonic tissues of Aedes aegypti (Diptera: Culicidae). , Biomedica: Vol. 25 No. 1 (2005)
- Érika Patricia Alarcón, Ángela María Segura, Guillermo Rúa-Uribe, Gabriel Parra-Henao, Ovitraps evaluation for surveillance and control of Aedes aegypti in two urban settlements of Urabá, Antioquia , Biomedica: Vol. 34 No. 3 (2014)
- Marcela Quimbayo, Guillermo Rúa-Uribe, Gabriel Parra-Henao, Carolina Torres, Evaluation of lethal ovitraps as a strategy for Aedes aegypti control , Biomedica: Vol. 34 No. 3 (2014)
- Jorge Mario Cadavid, Guillermo Rúa, Omer Campo, Gabriel Bedoya, Winston Rojas, Microgeographic and temporal genetic changes of Aedes aegypti from Medellín, Colombia , Biomedica: Vol. 35 No. 1 (2015)
| Article metrics | |
|---|---|
| Abstract views | |
| Galley vies | |
| PDF Views | |
| HTML views | |
| Other views | |
