High frequency of chromosome aberrations observed in lymphocytes in postmenopausal obese women
Abstract
Introduction. Epidemiological studies indicate that obesity is associated with an increased risk of 20-25% with several types of cancer.
Objective. The frequency of chromosome aberrations was evaluated in lymphocytes frompostmenopausal obese and non-obese women.
Materials and methods. Twenty obese and 20 non-obese women, all post-menopause, were recruited.The groups were matched according to age (± 5 years) and place of origin. After signing the consentform, women were interviewed using a structured questionnaire, and a blood sample (5 ml) was drawninto vacutainer tubes. From each sample, lymphocyte cell cultures were established with and without mitomycin C (challenge assay). Afterwards, the frequency of chromosome aberrations were recordedfor each group and treatment. Data were analyzed using the statistical program SPSS, v. 14.0.
Results. Obese women had a higher frequency of chromosome aberrations when compared with nonobesewomen. After exposing the cell cultures to mitomycin C, obese women presented an increasein the number of total chromosome aberrations in comparison to non-obese women (3.7± 0.6 vs.2.70±0.6; p=0.001).
Conclusions. The higher frequency of chromosome aberrations in lymphocytes from postmenopausalobese women compared to non-obese women suggested differences in the DNA repair capacity. Thismay indicate an association between genomic instability and the higher incidence of cancer in thispopulation.
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Chockalingam A. Healthy weight-healthy blood pressure. Can J Cardiol. 2010;26:259-60. http://dx.doi.org/10.1016/S0828-282X(10)70380-9
Lawrence JM, Mayer-Davis EJ, Reynolds K, Beyer J, Pettitt DJ, D’Agostino RB Jr, et al. Diabetes in Hispanic American youth: Prevalence, incidence, demographics, and clinical characteristics: The SEARCH for diabetes in youth study. Diabetes Care. 2009;32:S123-32. http://dx.doi.org/10.2337/dc09-S204
Hirani V. Generalized and abdominal adiposity are important risk factors for chronic disease in older people: Results from a nationally representative survey. J Nutr Health Aging. 2011;15:469-78.
Bhattacharya J, Sood N. Who pays for obesity? J Econ Perspect. 2011;25:139-58. http://dx.doi.org/10.1257/089533011798837800
Akil L, Ahmad HA. Effects of socioeconomic factors on obesity rates in four southern states and Colorado. Ethn Dis. 2011;21:58-62.
Finkelstein EA, Ruhm CJ, Kosa KM. Economic causes and consequences of obesity. Annu Rev Public Health. 2005;26:239-57.
Wiggins MS, Simonavice EM. Cancer prevention, aerobic capacity, and physical functioning in survivors related to physical activity: A recent review. Cancer Manag Res. 2010;2:157-64.
Finkelstein EA, Strombotne KL. The economics of obesity. Am J Clin Nutr. 2010;91:1520S-4S. http://dx.doi.org/10.3945/ ajcn.2010.28701E
Polednak AP. Trends in incidence rates for obesity-associated cancers in the US. Cancer Detect Prev. 2003;27:415-21. http://dx.doi.org/10.1016/j.cdp.2003.09.002
Duffey KJ, Popkin BM. High-fructose corn syrup: Is this what’s for dinner? Am J Clin Nutr. 2008;88:1722S-32. http://dx.doi.org/10.3945/ajcn.2008.25825C
Cuevas A, Álvarez V, Carrasco F. Epidemic of metabolic syndrome in Latin America. Curr Opin Endocrinol Diabetes Obes. 2011;18:134-8. http://dx.doi.org/10.1097/MED.0b013e3283449167
Jacoby E. The obesity epidemic in the Americas: Making healthy choices the easiest choices. Rev Panam Salud Pública. 2004;15:278-84. http://dx.doi.org/10.1590/S1020-
López-Jaramillo P, Lahera V, López-López J. Epidemic of cardiometabolic diseases: A Latin American point of view. Ther Adv Cardiovasc Dis. 2011;5:119-31. http://dx.doi.org/10.1177/1753944711403189
Gilbert-Diamond D, Baylin A, Mora-Plazas M, Villamor E. Correlates of obesity and body image in Colombian women. J Womens Health. 2009;18:1145-51. http://dx.doi.org/10.1089/jwh.2008.1179
Faeh D, Braun J, Tarnutzer S, Bopp M. Obesity but not overweight is associated with increased mortality risk. Eur J Epidemiol. 2011;26:647-55. http://dx.doi.org/10.1016/j.
leukres.2011.12.020
Bianchini F, Kaaks R, Vainio H. Weight control and physical activity in cancer prevention. Obes Rev. 2002;3:5-8. http://dx.doi.org/10.1046/j.1467-789X.2002.00046.x
Zeng H, Lazarova DL. Obesity-related colon cancer: Dietary factors and their mechanisms of anticancer action. Clin Exp Pharmacol Physiol. 2011;39:161-7. http://dx.doi.org/10.1111/j.1440-1681.2011.05518.x
Teras LR, Goodman M, Patel AV, Diver WR, Flanders WD, Feigelson HS. Weight loss and postmenopausal breast cancer in a prospective cohort of overweight and obese US women. Cancer Causes Control. 2011;22:573-9. http://dx.doi.org/10.1007/s10552-011-9730-y
Watson CS, Bulayeva NN, Wozniak AL, Finnerty CC. Signaling from the membrane via membrane estrogen receptor-alpha: Estrogens, xenoestrogens, and phytoestrogens. Steroids. 2005;70:364-71. http://dx.doi.org/10.1016/j.steroids.2005.03.002
Petrelli JM, Calle EE, Rodríguez C, Thun MJ. Body mass index, height, and postmenopausal breast cancer mortality in a prospective cohort of US women. Cancer Causes Control. 2002;13:325-32. http://dx.doi.org/10.1023/A:1015288615472
Rodríguez C, Calle EE, Fakhrabadi-Shokoohi D, Jacobs EJ, Thun MJ. Body mass index, height, and the risk of ovarian cancer mortality in a prospective cohort of postmenopausal women. Cancer Epidemiol Biomarkers Prev. 2002;11:822-8.
Liehr JG. Is estradiol a genotoxic mutagenic carcinogen? Endocr Rev. 2000;21:40-54. http://dx.doi.org/10.1210/er.21.1.40
Fogelholm M. Challenges in studies on resistance training and obesity. Obes Rev. 2008;9:88-9. http://dx.doi.org/10.1111/j.1467-789X.2007.00404.x
Jee SH, Kim HJ, Lee J. Obesity, insulin resistance and cancer risk. Yonsei Med J. 2005;46:449-55. http://dx.doi.org/10.3349/ymj.2005.46.4.449
Schindler AE. Progestogen deficiency and endometrial cancer risk. Maturitas. 2009;62:334-7. http://dx.doi.org/10.1016/j.maturitas.2008.12.018
Kaaks R, Lukanova A, Kurzer MS. Obesity, endogenous hormones, and endometrial cancer risk: A synthetic review. Cancer Epidemiol Biomarkers Prev. 2002;11:1531-43.
Lukanova A, Kaaks R. Endogenous hormones and ovarian cancer: Epidemiology and current hypotheses. Cancer Epidemiol Biomarkers Prev. 2005;14:98-107.
Maffini MV, Calabro JM, Soto AM, Sonnenschein C. Stromal regulation of neoplastic development: Agedependent normalization of neoplastic mammary cells by mammary stroma. Am J Pathol. 2005;167:1405-10. http://dx.doi.org/10.1016/S0002-9440(10)61227-8
Skorski T. BCR/ABL regulates response to DNA damage: The role in resistance to genotoxic treatment and in genomic instability. Oncogene. 2002;21:8591-604.
Huppi K, Pitt J, Wahlberg B, Caplen NJ. Genomic instability and mouse microRNAs. Toxicol Mech Methods. 2011;21:325-33. http://dx.doi.org/10.3109/15376516.2011.562759
Beckman RA, Loeb LA. Genetic instability in cancer: Theory and experiment. Semin Cancer Biol. 2005;15:423-35. http://dx.doi.org/10.1016/j.semcancer.2005.06.007,
Au WW, Oberheitmann B, Harms C. Assessing DNA damage and health risk using biomarkers. Mutat Res. 2002;509:153-63. http://dx.doi.org/10.1016/S0027-5107(02)00226-9
Au WW, Salama SA. Cytogenetic challenge assays for assessment of DNA repair capacities. Methods Mol Biol. 2006;314:25-42. http://dx.doi.org/10.1385/1-59259-973-7:025
Lin J, Zhang X, Chen Y. Mutagen sensitivity as a susceptibility marker for endometriosis. Hum Reprod. 2003;18:2052-7. http://dx.doi.org/10.1093/humrep/deg393
Rossner P, Boffetta P, Ceppi M, Bonassi S, Smerhovsky Z, Landa K, et al. Chromosomal aberrations in lymphocytes of healthy subjects and risk of cancer. Environ Health Perspect. 2005;113:517-20. http://dx.doi.org/10.1289/ehp.6925
World Medical Association Declaration of Helsinki. Ethical principles for medical research involving human subjects. Nurs Ethics. 2002;9:105-9.
Álvarez-Rosero RE, Rodríguez-Argote J, Arboleda- Moreno YY, Muñoz-Benítez SL, Sierra-Torres CH. Chromosome aberrations in peripheral blood lymphocytes of high-risk HPV-infected women with HGSIL. Environ Mol Mutagen. 2008;49:688-94. http://dx.doi.org/10.1002/em.20418
Goto K, Akematsu T, Shimazu H, Sugiyama T. Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining. Chromosoma. 1975;53:223-30. http://dx.doi.org/10.1007/BF00329173
An International System for Human Cytogenetic Nomenclature (1985) ISCN 1985. Report of the Standing Committee on Human Cytogenetic Nomenclature. Birth Defects Orig Artic Ser. 1985;21:1-117.
Whorton EB Jr. Some experimental design and analysis considerations for cytogenetics studies. Environ Mutagen. 1985;7:9-15. http://dx.doi.org/10.1002/em.2860070804
Hodge FS, Cantrell BG, Kim S. Health status and sociodemographic characteristics of the morbidly obese American Indians. Ethn Dis. 2011;21:52-7.
Hetherington MM, Cecil JE. Gene-environment interactions in obesity. Forum Nutr. 2010;63:195-203. http://dx.doi.org/10.1159/000264407
Chateau-Degat M-L, Dewailly E, Charbonneau G, Laouan-Sidi EA, Tremblay A, Egeland GM. Obesity risks: Towards an emerging Inuit pattern. Int J Circumpolar Health.
;70:166-77.
Sánchez-Johnsen LA, Fitzgibbon ML, Martinovich Z, Stolley MR, Dyer AR, van Horn L. Ethnic differences in correlates of obesity between Latin-American and black Women. Obes Res. 2004;12:652-60.
American Cancer Society. Does body weight affect cancer risk? Fecha de consulta: 12 de julio de 2011. Disponible en: http://www.cancer.org/cancer/cancercauses/
dietandphysicalactivity/bodyweightandcancerrisk/bodyweight-and-cancer-risk-effects.
Pierce MB, León DA. Age at menarche and adult BMI in the Aberdeen children of the 1950s cohort study. Am J Clin Nutr. 2005;82:733-9.
Bratberg GH, Nilsen TIL, Holmen TL, Vatten LJ. Early sexual maturation, central adiposity and subsequent overweight in late adolescence. A four-year follow-up of
,605 adolescent Norwegian boys and girls: The Young HUNT study. BMC Public Health. 2007;7:54. http://dx.doi.org/10.1186/1471-2458-7-54
Correia LL, da Silveira DMI, e Silva AC, Campos JS, Machado MM, Rocha HA, et al. Prevalence and determinants of obesity and overweight among reproductive age women living in the semi-arid region of Brazil. Cien Saude Colet. 2011;16:133-45. http://dx.doi.org/10.1590/S1413-81232011000100017
Mesa JM, Martínez J, Araújo C, Horta BL, Gigante DP. Growth patterns in early childhood and the onset of menarche before age twelve. Rev Saude Publica. 2010;44:249-60.
http://dx.doi.org/10.1590/S0034-89102010000200004
Emaus A, Espetvedt S, Veierød MB, Ballard-Barbash R, Furberg A-S, Ellison PT, et al. 17-beta-estradiol in relation to age at menarche and adult obesity in premenopausal
women. Hum Reprod. 2008;23:919-27. http://dx.doi.org/10.1093/humrep/dem432
He C, Kraft P, Chasman DI, Buring JE, Chen C, Hankinson SE, et al. A large-scale candidate gene association study of age at menarche and age at natural menopause. Hum
Genet. 2010;128:515-27. http://dx.doi.org/10.1007/s00439-
-0878-4
Cooper GS, Baird DD, Darden FR. Measures of menopausal status in relation to demographic, reproductive, and behavioral characteristics in a population-based study of
women aged 35-49 years. Am J Epidemiol. 2001;153:1159-65. http://dx.doi.org/10.1093/aje/153.12.1159
Schumann B, Kluttig A, Tiller D, Werdan K, Haerting J, Greiser KH. Association of childhood and adult socioeconomic indicators with cardiovascular risk factors and
its modification by age: The CARLA Study 2002-2006. BMC Public Health. 2011;11:289. http://dx.doi.org/10.1186/1471-2458-11-289
Urquhart CS, Mihalynuk TV. Disordered eating in women: Implications for the obesity pandemic. Can J Diet Pract Res.2011;72:50.
Anderson-Bill ES, Winett RA, Wojcik JR. Social cognitive determinants of nutrition and physical activity among webhealth users enrolling in an online intervention: The influence of social support, self-efficacy, outcome expectations, and self-regulation. J Med Internet Res. 2011;13:e28. http://dx.doi.org/10.2196/jmir.1551
Ujcic-Voortman JK, Bos G, Baan CA, Verhoeff AP, Seidell JC. Obesity and body fat distribution: Ethnic differences and the role of socio-economic status. Obes Facts. 2011;4:53-60. http://dx.doi.org/10.1159/000324555
Bulló M, García-Aloy M, Martínez-González MA, Corella D, Fernández-Ballart JD, Fiol M, et al. Association between a healthy lifestyle and general obesity and abdominal
obesity in an elderly population at high cardiovascular risk. Prev Med. 2011;53:155-61. http://dx.doi.org/10.1016/j.ypmed.2011.06.008
Moktar A, Singh R, Vadhanam MV, Ravoori S, Lillard JW, Gairola CG, et al. Cigarette smoke condensate-induced oxidative DNA damage and its removal in human cervical
cancer cells. Int J Oncol. 2011;39:941-7. http://dx.doi.org/10.3892/ijo.2011.1106
Friedenreich CM, Orenstein MR. Physical activity and cancer prevention: Etiologic evidence and biological mechanisms. J Nutr. 2002;132:3456S-64S.
Al-Zadjali M, Keller C, Larkey LK, Albertini L. Evaluation of intervention research in weight reduction in post menopausal women. Geriatr Nurs. 2010;31:419-34. http://
dx.doi.org/10.1016/j.gerinurse.2010.08.010
Natarajan TG, Ganesan N, Carter-Nolan P, Tucker CA, Shields PG, Adams-Campbell LL. Gamma-radiationinduced chromosomal mutagen sensitivity is associated with breast cancer risk in African-American women: Caffeine modulates the outcome of mutagen sensitivity assay. Cancer Epidemiol Biomarkers Prev. 2006;15:437-42. http://dx.doi.org/10.1158/1055-9965.EPI-05-0353
Pavanello S, Clonfero E. Individual susceptibility to occupational carcinogens: The evidence from biomonitoring and molecular epidemiology studies. G Ital Med Lav Ergon.
;26:311-21.
Au WW. Abnormal chromosome repair and risk of developing cancer. Environ Health Perspect. 1993;101:303-8.
Pechère-Bertschi A, Burnier M. Female sex hormones, salt, and blood pressure regulation. Am J Hypertens. 2004;17:994-1001. http://dx.doi.org/10.1016/j.amjhyper.2004.08.009
Payer J, Jackuliak P, Nagyová M. Obesity and a risk of carcinoma. Vnitr Lek. 2010;56:1082-7.
Schindler AE. Progestogen deficiency and endometrial cancer risk. Maturitas. 2009;62:334-7. http://dx.doi.org/10.1016/j.maturitas.2008.12.018
Yang HP, Black A, Falk RT, Brinton LA, Potischman N, Wentzensen N, et al. Association of serum sex steroid hormone hemodilution and body mass index among healthy postmenopausal women. Ann Epidemiol. 2011;21:466-71. http://dx.doi.org/10.1016/j.annepidem.2011.01.003
La Vecchia C, Giordano SH, Hortobagyi GN, Chabner B. Overweight, obesity, diabetes, and risk of breast cancer: Interlocking pieces of the puzzle. Oncologist. 2011;16:726-9.
http://dx.doi.org/10.1634/theoncologist.2011-0050
Hu F, Liu F. Mitochondrial stress: A bridge between mitochondrial dysfunction and metabolic diseases? Cell Signal. 2011;23:1528-33. http://dx.doi.org/10.1016/j.cellsig.
05.008
Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, Esquivel-Soto J, Morales-González A, Esquivel-Chirino C, et al. Inflammation, oxidative stress, and obesity. Int J
Mol Sci. 2011;12:3117-32. http://dx.doi.org/10.3390/ijms 12053117
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